Biological molecules that possess catalytic activity. They may occur naturally or be synthetically created. Enzymes are usually proteins, however... | Contact experts in Enzymes to get answers
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Questions related to Enzymes
looking for the relation of biogenic amine synthesis with lactobacillus bacteria. which lactobacillus strain is responsible for synthesizing biogenic amines, and how the metabolism process is...
23 July 2022 4,862 2 View
I was slowly raised temperature from 0 to 277K, and it was equaliibration by 400ps. During equilibration, the structure did not change, but I found that 100 ns DNA structure was changing. What...
22 July 2022 1,437 0 View
I am trying to establish a control probe for RNA in situ hybridization for human samples. The plan is to synthesize DIG-labeled probes by in vitro transcription. Does anyone have primer sequences...
18 July 2022 5,298 0 View
I am currently developing a LCMS method for certain genotoxic impurity in API on a shimadzu 9030 qTOF LCMS. And I had an argument with my coworker about which mode is more sensitive, SIM or...
09 July 2022 1,856 4 View
Hello, please provide me with some information if you have studied or conducted experiments on the use of pectinase enzyme in plant cell suspension.
07 July 2022 4,313 0 View
Molecular Dynamics, PDB
30 June 2022 8,569 0 View
I have seen that > 100ng is a typical minimum from many WGS providers. However, I likely have less than this? What method, platform or company would be best for performing whole genome sequencing...
22 June 2022 7,818 1 View
Hello everyone! Can anyone suggest me an appropriate way of making glucose standards using DNS solution for cellulase activity quantification?.
21 June 2022 1,392 1 View
Hi, we are trying to transfect breast cancer cell lines with several cell penetrating peptide-siRNA complexes but it seems that our peptides are not taken up by the cells. We use several CPPs:...
20 June 2022 2,844 0 View
This question is about the mixture of two diastereomers isolated as a single compound.
16 June 2022 4,776 1 View
Hello, currently I am doing a recombinant protein expression on E. coli BL21. For my research I am checking the extracellular for the targeted protein. Currently the expression and purification...
14 June 2022 2,632 3 View
Hi everyone!! Help me My protein has size about 65kD. The plasmid is pET22b. After I induced by the IPTG concentration of 1mM, 37 degree C, then my strain failed to grow. And I broke the cell for...
06 June 2022 9,232 0 View
gene editing in wheat
02 June 2022 10,005 4 View
Hi, We accidentally bought Nessler's Reagent A (potassium tetraiodomercurate(II) solution) instead of Nessler's Reagent (for detection of ammonia). I understand that we can add NaOH at a certain...
29 May 2022 7,916 4 View
I need the Toxic response factor for Mn, Fe, Al, Mo and Co for my research, as far as I know Hakanson, 1980 reported it just for 8 heavy metals!
27 May 2022 9,408 2 View
I need to determine them for Degree of gelatinization and retrogradation.
17 May 2022 8,996 1 View
What do you think about the current energy transition? Are all the countries ready to forgo the fossil fuels and shift to renewable energy? Will this energy transition help to fulfill the goals of...
15 May 2022 5,154 10 View
So I have a GC method with ethyl chloroformate that works for almost all amino acids except it doesn't do arginine, and serine and threonine smear badly due to the active hydroxyls. I tried OPA...
12 May 2022 9,368 4 View
Some claim that fasting is spiritually beneficial for appeasement and atonement, among others. The question is "what are the health benefits of fasting?" Thanks for your contributions.
24 April 2022 7,020 39 View
Does anyone have an idea of a good lysis buffer detergent for membrane protein purification? I am working on purifying a single-span membrane protein, and I have tried SDS, IGEPAL, Triton, and...
21 April 2022 5,538 4 View
I am working on seaweed polysaccharides and I conduct proton and C13 NMR analysis for the characterization of these polymers. So is there any good software that can interpret the NMR...
17 April 2022 2,011 5 View
Hi everyone I need some good papers which can explain the above-asked question. I need to know what exactly LRCs tell about the photosynthesis in plants which is not covered by chlorophyll...
16 April 2022 5,999 3 View
I am carrying out acetylation reaction of L-lysine, during work up i am getting strong frothing/foaming and some times reaction mass coming out from the flask. This phenomena not observed...
15 April 2022 4,412 4 View
Recently, phages are used against the MDR pathogen in humans to combat the diseases. Wouldn’t our immune system try to eliminate those bacteriophages? How these techniques are successful?
15 April 2022 2,428 7 View