Biological molecules that possess catalytic activity. They may occur naturally or be synthetically created. Enzymes are usually proteins, however... | Contact experts in Enzymes to get answers
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Questions related to Enzymes
Hello all, I am trying to transfect my bacmid to sf-21 cell line for baculovirus generation and I am facing a bacterial contamination in the transfected well. I used alkaline lysis method to...
16 May 2024 3,527 1 View
Probably this question seems fool to you, but I am quite new in the research area and there are a lot of experiments I have never done. My question regards the amount of buffer, enzyme, incubation...
16 May 2024 3,962 1 View
I did qualitative assay on agar plates with starch as sole carbon source for checking amylase activity, once I added iodine the clear zone appeared arround the colonies but after some hours and...
14 May 2024 6,501 2 View
Hello I'm measuring SOD activity in plant leaves using NBT method. Reaction solution contains EDTA, riboflavin, methionine and PBS. But my samples values are higher than control. Do you know how I...
10 May 2024 8,913 1 View
It is about my research using a orange peel pectin derived agar in cultivating bacteria that is very versatile and can grow on wide variety of conditions, can you suggest me a gram positive...
10 May 2024 7,356 0 View
What are the primary differences between Syndrome of Inappropriate Antidiuretic Hormone (SIADH) and Diabetes Insipidus (DI)?Advances in Understanding and Management of Antidiuretic Hor...
09 May 2024 5,736 3 View
When I refined my XRD data by using GSAS software, the GOF value always came out at more than 4. My material is an O3-type layered transition metal oxide cathode material.
09 May 2024 7,361 3 View
Forthcoming Springer book: Genome Editing for Sustainable Agriculture Book Series Vol 1 Plant Genome Editing Development and Technologies (Springer) edited by Khaled F. M. Salem, Jameel M....
09 May 2024 5,002 0 View
I have energy minimized my structure using gromacs for 20 ps following the lysozyme tutorial. I did 3 such replicates for the minimization. The potential energy for all these 3 replicates was the...
06 May 2024 7,770 4 View
How do i refine my search for information in your database. I am looking for peer reviewed journals associated with omnichannel banking only.
05 May 2024 7,378 1 View
I am following the standard method which is mentioned in Tomotake Kanki et al., 2009 research paper Monitoring mitophagy in yeast: The Om45-GFP processing assay . I am growing the cells in SD-HIS...
05 May 2024 6,440 0 View
We have an Online International Multidisciplinary Journal, however, it is not yet indexed in Scopus and in Web of Science, what's the fastest way to have it indexed in this prestigious indexing...
01 May 2024 5,262 0 View
I am preparing an experiment in which I would like to sample roots of sunflowers grown in pots (substrate is soil) in later stages of the plant development. Can anyone tell me, what is the best...
30 April 2024 8,751 1 View
I would like to use the "create.matrix" function (R package "fossil") to create a matrix of taxa, localities, time and abundance. In the function, time is specified by the arguments "time.col" (=...
29 April 2024 3,523 2 View
I want to stain acute brain slices with calcium orange and DAPI, but I am not sure whether these two kinds of dye can be added together or not.
29 April 2024 942 1 View
good greetings I need references such as books and papers about artificial intelligence in international law.
28 April 2024 2,693 1 View
greetings, I'm culturing SW480 cells on DMEM high glucose gibco with FBS. When I'm performing MTT Assay, upon treatment of cells with Baicalein, the color of medium with 5% FBS changes to dark...
26 April 2024 7,099 4 View
Describe the role of decompressive craniectomy in the management of refractory intracranial hypertension. Optimizing Traumatic Brain Injury Management: A Multidiscipl...
25 April 2024 685 1 View
The culture was pure (there was one colony type on MRS solid medium). I did Gram staining on one colony and I found the two morphological types. Many trials were carried out and I got the same...
25 April 2024 6,003 0 View
Hello everyone. I'm trying to see secreted-phosphorylated-protein by western blot, but struggling with detecting it. There's nothing wrong with detecting whole-cell lysate samples but it's...
24 April 2024 135 0 View
I am a Nigerian researcher working on microbial diversity of an African fermented food product. I will appreciate it if l can get a location and price for shotgun metagenomic sequencing.
21 April 2024 2,517 2 View
I am a first year Ph.D. student in Cell and Molecular Biology department, UT. I am going to start a project on Breast Cancer and for this I need to be skilled on cell culture. I never did cell...
18 April 2024 6,530 7 View
I have two groups that I collected microglia from and did RTqPCR. However, the CT values come up as extremely different (p
16 April 2024 6,239 0 View
Hi everybody, I want to know if some DNA sequences form G4 in cell. They are 40 to 80 nt long and not part of a promoter. The reporter assays I found in literature evaluate G4 forming in...
16 April 2024 6,826 0 View