Biological molecules that possess catalytic activity. They may occur naturally or be synthetically created. Enzymes are usually proteins, however... | Contact experts in Enzymes to get answers
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Questions related to Enzymes
I have had a large number of citations and felt my H-index would be 20 at this time?
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I am preparing a study to review dietary intervention and the peritoneal dialysis effluent microbiome of peritoneal dialysis patients. I was wondering the quantity of the effluent needed for...
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One of the major stumble points in research has to do with Exceptions. On one hand, Exceptions hint about interesting hidden phenomena that may be relevant and therefore should be included in the...
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Ms_JOBAN_13551 : Invitation to Review Manuscript for Journal of Biology and Nature.
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So I've been experimenting on HEK293T cells and they seem to have been growing on top of each other (to an extent) and now on ImageJ some of the really bright hoerscht signal is overlapping and...
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Hi, Elsevier has charged my corporate credit card for the purchase of an article that I did not buy. There is no record of it under my profile, but yet I hard a charge, and Elsevier has been...
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Hi everyone! We would like to perform this protein synthesis in my lab in order to then purify it for hybridomas development in mouse. We have found a plasmid that suits our requirements (AmpR,...
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Hi all, I am currently investigating the effects of glucagon signaling on PKA activation in hepatocytes with Glucagon (1-29), bovine, human hydrochloride (HY-P0082A). Specifically, I would like to...
last month 8,255 1 View
The IC50 calculation was done on human cells line and I want calculate LD50 for mice.
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In our current work, we are exploring the possibility that fermion rest mass originates not from an external scalar (Higgs-like) mechanism, but from internal spinor structuration — what we call...
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I have an in vivo experiment in mice, in which I want to inject Olaparib ( HY-10162, MCE) intra-peritoneal. To prepare Olaparib, I first need to suspend it in DMSO, then add 20% SBE-β-CD...
last month 6,758 1 View
Hello, I am trying to conduct CO-IP experiment. I have IP: IgG , IP: Protein-A, and input. I am performing western for Protein-B. My results seem to get a band at the expected size in input , IP:...
11 August 2025 4,387 3 View
We plan to dissolve the enzyme (HY-P2863,MCE) in ultrapure water to create a concentrated stock solution, as recommended. We will then prepare small aliquots of this stock, freeze them at -20°C,...
08 August 2025 384 1 View
Is there any published validation for Androscope (portable CASA) assessment in dog semen?
07 August 2025 2,621 0 View
Hi everyone, I’m currently performing retroviral transduction of primary T cells using viral supernatant generated from Plat-E (Platinum-E) packaging cells. I’m using Retronectin-coated plates to...
06 August 2025 3,544 1 View
In experiments involving co-infection of A549 cells with M. abscessus-mCherry and P. aeruginosa PAO1-GFP, what combination of assays is recommended to conclusively distinguish between bacteria...
06 August 2025 4,045 1 View
Have you ever read the article below? There is a no yet known critical problem-solving impossibility theorem, which indicates that it is not possible to solve a problem just by managing its...
05 August 2025 8,443 4 View
Oestrogen (a female sex hormone), lifestyles, behaviours, and tumour characteristics support the lower incidence of CRC in females.
05 August 2025 950 5 View
I want to check peptide specificity with EGFR. I think that peptide should internalize through EGFR in EGFR positive cell lines. I took A549 and Cal 33 cell line, both are EGFR positive cell line....
04 August 2025 5,761 2 View
Hello everyone. I have isolated four different bacteriophages against four different Staphylococcus aureus strains. In all, the plaque sizes on double agar assay are very small (around 0.5-1.0...
31 July 2025 6,283 1 View
Just for the information to about it
29 July 2025 4,739 1 View
I have 25% ammonia solution, I want to prepare 500mM Ammonium hydroxide solution (suppose 1 Litre), How much volume (quantity) of 25% ammonia solution I should take?
28 July 2025 756 0 View
Hi all, can PolyFast (HY-K1014, MCE) be used to transfect suspension cells?
28 July 2025 4,753 1 View
I amplified my DNA insert for cloning and confirmed the product by agarose gel electrophoresis. The gel showed a clear, single band at approximately 1.4 kb, which matches the expected size of my...
28 July 2025 5,356 7 View