Biological molecules that possess catalytic activity. They may occur naturally or be synthetically created. Enzymes are usually proteins, however... | Contact experts in Enzymes to get answers
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Questions related to Enzymes
A lot of answers/literature online say that T4 ligation of >3 fragments is very difficult/occurs at low efficiency. However, I also see reports/protocols everywhere of Golden Gate being used to...
20 September 2021 7,100 2 View
Hi everyone. I would like to know if anyone knows a way to improve a recombination reaction using the Gateway™ LR Clonase™ II Enzyme mix. I was thinking in adding ATP, but I don't know if the...
20 September 2021 5,630 1 View
Does anyone know how many of each is in this lysing matrix and the best way to make this yourself? Components: 1.4mm ceramicspheres, 0.1mm silica spheres 4 mm glass bead.
17 September 2021 2,630 0 View
Hi there, I am having problems with 50ul Matrigel domes completely dissolving in 24 well plates. I am trying to generate organoid cultures, but when I remove the culture media after 2 days the...
15 September 2021 8,465 5 View
I am looking for places to purchase lentiviral vectors for clinical use as a part of the CAR T cell therapy protocol, any recommendations would be appreciated.
13 September 2021 5,474 1 View
Hi everybody, I need to screen pro-apoptotic factors released by cells in the culture medium (treatment vs control group). Ideally, I am looking for a protein array that allows screening for...
12 September 2021 4,355 1 View
Kindly guide me.
08 September 2021 5,306 4 View
I've done molecular docking using AutoDock Vina and analyzed the protein-ligand interaction using Ligplot+. I have observed that the ligands I used with good binding energy (negative) have poor...
06 September 2021 7,822 3 View
I performed ABTS assay to detect laccase activity but i could not find the change in color of ABTS from light green to dark green. It changed to colorless. Does anyone have an idea ? Please...
02 September 2021 9,251 14 View
Wanted to take lambda max through UV for compounds soluble only in chloroform and DMSO. Used PO4 buffer as co-solvent, But the compound seemed to show turbidity when the buffer is added and there...
01 September 2021 7,792 0 View
The following data are obtained in an enzyme catalyzed reaction: [S]x10^4(moldm^-3): 2.5, 5.0, 10.0, 15.0. Then rox10^6(moldm^-3min^-1) : 2.2, 3.8, 5.9, 7.1. Assuming Michaelis-Menten Kinetics,...
26 August 2021 7,302 3 View
Dear all, Im new here and I need help from you all. How to inactivated restriction enzyme if the enzyme can't be heated inactivated? Such as sfiI from ThermoFisher. Please answer me. Thank you.
24 August 2021 895 5 View
Dear researchers, I used Agrobacterium tumefaciens to transform Arabidopsis thaliana, and the vectors were constructed on pCAMBIA1301 and pCAMBIA1302 with a hygromycin resistance gene on them. I...
22 August 2021 1,233 26 View
I want to test Beta-galactosidase (purified protein) activity using pNPG. I have always used oNPG. There is any difference? Should I change the buffer or I can use Sodium Phosphate? Should I...
18 August 2021 9,957 0 View
I setup an electro blotting experiment for overnight wet transfer using Tris glycine based transfer buffer at 20V. Next day , to my surprise there was lots of rusty froth in the tank. Also their...
17 August 2021 4,274 2 View
I have tried to break DNA by water sonicator, but after gel run i saw that nothing happen. I took 300 μl DNA in eppendrop and flaot it on the bath, temp was 28-34`C, i sonicate the sample in three...
16 August 2021 4,712 4 View
This is SEM image of cross section of Sn/Pb solder joint stored in dry ice for 500h. Some Sn spheres can be seen near the corner of the solder joints. Can someone teach me what is the mechanisms?...
13 August 2021 8,089 2 View
kindly explain the relative and residual activity of enzyme with formula
11 August 2021 7,904 5 View
I want to hydrolyze oat protein by using enzyme Alcalase 2.4 L ( 2.4 AU g). how can we calculate enzyme / substrate ratio 0.3 AU g of protein??
10 August 2021 5,063 3 View
I would like to know if it's possible to use Hi-T4™ DNA Ligase for making a homemade Gibson assembly Mastermix. I found that Hi-T4 ligase is Thermotolerant and can ligate sticky ends, but it's not...
02 August 2021 8,390 2 View
I want to study the activity of laccase. All literature are using extinction coefficient values (epsilon) for laccase substrates i.e: syringaldazine (SGZ), dimethoxyphenol (2,6-DMP),... Despite...
30 July 2021 7,377 8 View
I am interested how to find aggregation rate using dynamic light scattering data? I can obtain hydrodynamic radius dependence on time. I need not only answer but sources too.
22 July 2021 5,591 6 View
I have a target and a small molecule which is interaction with the enzyme. How do you determine whether the small molecule is a substrate or an competitive inhibitor of the enzyme? I feel so...
21 July 2021 1,356 5 View
Hhal restriction site is G CG
21 July 2021 5,141 1 View