Biological molecules that possess catalytic activity. They may occur naturally or be synthetically created. Enzymes are usually proteins, however... | Contact experts in Enzymes to get answers
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Questions related to Enzymes
Hi, In the process of histidine metabolism 4-imidazolone 5-propionate gets converted into N-Formimimino Glutamate (FIGLU) with the help of 4-imidazolone 5-propionase enzyme. I want to know whether...
29 March 2021 5,355 3 View
We are trying to establish a isomerisation process to convert sucrose to isomaltulose. There are scientific papers that have claimed the construction of special enzymes using recombinant gene...
29 March 2021 5,943 3 View
I would like to do method validation for co-trimoxazole tablet but couldn't find the proper neutralizer to deactivate co-trimoxazole active. Without deactivating i cant get bacteria,yeast and...
26 March 2021 7,408 2 View
I'm new to enzymes and I would like to receive all possible help for this. I have a product that initially contains 57.9g of starch, which I would like to hydrolyze with alpha amylase, that has an...
26 March 2021 2,769 3 View
I am doing biochemical characteristics of 2 cysteine proteases (A and B). However, the substrate specificity of enzyme B is very different (Enzyme A worked as I expected but B showed different...
22 March 2021 4,978 5 View
I come from a chemistry background and so I’m used to thinking in terms of proportions, so forgive me if my thinking is completely wrong. *** I’d like to simulate a reaction that occurs in the...
18 March 2021 1,058 10 View
I have a dataset with ordinal variables (Likert scale), I need to find the relation between variables. Only by curiosity, I have performed MCA and PCA. The literature recommends MCA for...
18 March 2021 9,468 6 View
Here are the details to the experiment Innoculum : Thickened Sludge Feed : Synthetic acetic acid and butyric acid Reactors : CSTR reactor with working volume of 11L pH at the start of my...
16 March 2021 5,857 3 View
Hi! I am working with Pichia pastoris (X-33 strain) on the synthesize of some recombinant serine proteases. I selected some clones from YPD-agar-Zeocin plates (zeocin concentration of 1000ug/mL)....
09 March 2021 3,124 1 View
I need to optimize a method where I utilize DNase I to remove unwanted DNA from lyzed epithlial cells, while leaving sperm cells intact in the cell suspension. I started with DNase I at 15U/uL,...
08 March 2021 8,244 2 View
I have been working with a system that has a transition metal (TM) and imidazole ligand. When ORCA comes to solve the CP-SCF equation, it performs the solution until a certain interaction but...
05 March 2021 6,135 4 View
I have a list of DEGs from microarray. I want to find gene networks that are included in the, for example, 100 top differentially expressed genes. What is the best approach to do so and draw the...
24 February 2021 7,335 3 View
I am trying to find the fraction metabolized of a drug by CYP and UGT enzymes. I am unable to get the values of fraction metabolized (Fm) directly from the literature. Can we calculate the Fm of a...
22 February 2021 5,646 2 View
I was wondering if AC16 recapitulate the basic electrophysiological features of cardiomyocytes? Are they excitable by field stimulation? Do they show calcium transients? Or can they be made...
19 February 2021 6,955 0 View
Hi everyone, We are doing a coculture of cancer cell lines and monocytes (THP-1). As a control, we need to cultivate THP-1 in the context of normal cells. For this experiment, we need to polarize...
17 February 2021 9,690 5 View
Hi, I'm asking from the point of view of designing a research plan: How long (approximately) can it take to determine the transition state structure of an enzyme substrate using kinetic isotope...
15 February 2021 2,776 1 View
Hello! I want to determine the activity of my in house Reverse Transcriptase enzyme. For this, I have used Thermo's Enzcheck Reverse Transcriptase assay kit. I used commercial Thermo RT enzyme to...
09 February 2021 7,477 1 View
I'm working on enzymes. There is a term “BU” in relation to enzyme performance, BU : one baker unit(BU) is the amount of catalase that will decompose 264mg of hydrogen peroxide under the...
08 February 2021 2,215 1 View
I am looking to calculate hedge's g effect sizes for a psychological intervention. A similar paper talks about obtaining "effect sizes by subtracting the posttest mean of the comparison group...
04 February 2021 9,934 5 View
I have used potassium ferricyanide (which is likely the most common) extensively in the past, but I am curious to know if there are other oxidants that work well. I need to generate the oxidized...
30 January 2021 9,962 4 View
I have many flasks of yeast culture (some of them treated with a vegetable peel), and I want to know which flask contains more yeast loads after incubation(starting with 10^8 yeast cells). Can I...
30 January 2021 3,492 7 View
I used to try 50 mM phosphate buffer pH 6.8 but it failed and 100 mM NaOH can dissolve but pH is over 6.8 .
28 January 2021 6,682 0 View
I want to calculate independent number of snps to set a threshold for GWAS. As the basic bonferroni correction is too conservative. Due to correlation between snps, we cannot assume they are...
27 January 2021 4,527 7 View
Good morning everyone, First of all, happy new year and best wishes! I would like to know if someone could help me to find “easily” pyr-GC-MS analysis data on plants? Do databases exist for this...
26 January 2021 5,594 2 View