Biological molecules that possess catalytic activity. They may occur naturally or be synthetically created. Enzymes are usually proteins, however... | Contact experts in Enzymes to get answers
2,319 views 5,360 posts
Questions related to Enzymes
Hello everyone, This involves New England Biolab kits. HiScribe® T7 ARCA mRNA Kit (without tailing) NEB #E2065S https://www.neb.com/en-us/products/e2065-hiscribe-t7-arca-mrna-kit HiScribe® T7...
10 February 2025 2,685 2 View
I need detail answer from your side seniors.
10 February 2025 4,112 1 View
Dear fellow researchers, Hello, I have been culturing bEnd3 cells recently. A few days ago, I coated a plate with 2% gelatin and seeded the cells. Most of the wells showed normal cell growth,...
08 February 2025 9,906 4 View
I'm trying to think through the best way to stimulate mouse CAR T cells that target mouse CD19 (1D3 scFv) without incubating the CAR T cells with B cells. I want to do this because a metabolic...
07 February 2025 5,534 1 View
Hello everyone, I am working on docking my recombinant protein with lipopolysaccharide (LPS) using in silico methods. However, I am having trouble finding a PDB file for LPS. I have checked the...
07 February 2025 5,204 2 View
I want to clone my 60 bp insert into my approximately 7000 bp vector. I did the ligation with a vector to insert ratio of 1:10. Either very few or no colonies grew. Also my colony PCR results were...
06 February 2025 9,530 4 View
This huge mushroom has sprouted on a dead, cut trunk of Conocarpus lancifolius from the Combretaceae family. It resembles Ganoderma, but I am not an expert in mushroom identification. Any help...
06 February 2025 4,815 4 View
I am trying to isolate membrane proteins from Nicotiana benthamiana, but I have encountered challenges with existing protocols. Many research papers mention the isolation process but lack specific...
04 February 2025 3,118 3 View
Hello everyone, Just a brief question: what would you suggest if I have a bunch of tissue samples floating in formalin for ~ a year. Mainly, I have two options: 1. go with freezing (wash from...
03 February 2025 2,363 2 View
Im doing research about gene fusion of 2 fragment ( 1000 bp and 500 bp ). I get single bright band from each gene fragment. I continue with 2 step PCR overlap PCR : first primerless PCR and the...
02 February 2025 9,733 2 View
I have plasmid expressed a lytic protein in bl21 cells and did a growth kinetics and post induction there is reduction in turbidity which continued for three hours but post five the culture...
02 February 2025 2,334 2 View
Current evidence suggests that the energy density of matter can be stored in the vacuum of the universe. the more the vacuum reigns, the more this energy density will be stored in the vacuum of...
01 February 2025 2,324 0 View
how do i prepare the various concentrations of an emulsion for drug treatment? i have prepared an oil in water emulsion encapsulating docetaxel. to treat my cells (mcf-7). I need to prepare serial...
31 January 2025 2,523 3 View
as there must be difference in steps between H&E Histology and flourescent microscopy for liver of rats
30 January 2025 4,719 2 View
Hi, can someone suggest a good free software for analyzing FCS files? I analyzed my data with the free trial of FCS Express. I need software to install on my personal computer. Thanks
30 January 2025 9,992 3 View
Hi there! I am trying to clone with the LR Clonase enzyme (LR Clonase II, Thermo Fisher). I have an entry vector for each gene of interest (genes with a length of 120 and 490 bp approximately),...
29 January 2025 9,257 3 View
When synthesizing MOFs, does the amount of solvent affect the produced material characteristic? and how to determine the best amount of solvent?
29 January 2025 1,373 2 View
I have had some problems subculturing mammalian cells in my cell culture room. (I ruled out most other options by sterilizing everything) . To spot the source of the contamination, I left some...
28 January 2025 8,297 0 View
Currently, I tried with siRNA using mobile phase A: 100mM TEAA+ Acetonitrile (20:80) and mobile phase B: 100mM TEAA+ Acetonitrile (95:5). But the RT keeps changing and baseline is not straight.
27 January 2025 6,858 0 View
For my research, it is necessary to find the width (in the figure, BE) of the failure wedge in Terzaghi, Vesic, or Prandtl bearing capacity theories for shallow foundations. I searched the...
25 January 2025 2,893 0 View
I am a student who wishes to connect with another researcher in order to clarify something but she does not follow me. how could i reach her?
24 January 2025 7,157 3 View
I am a PhD scholar working on Gestational diabetes mellitus model in C57BL/6J mice. Soon after the delivery, mothers will either kill their pups or do non-lactation/ failure to nurse and...
24 January 2025 4,082 0 View
I am working with cyanobacteria and to study changes at photosystems level I have performed BN-PAGE. But unlike the results I saw in other papers I do not get the images? So I am confused whether...
24 January 2025 4,569 3 View
thanks for your answers in advance
24 January 2025 192 3 View