Biological molecules that possess catalytic activity. They may occur naturally or be synthetically created. Enzymes are usually proteins, however... | Contact experts in Enzymes to get answers
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Questions related to Enzymes
Hi everyone We are about to start a project of local biodiversity monitoring by indigenous and cattle ranchers, and by what we have search, there is a scarcity of tecnical documents that describe...
23 November 2021 2,102 9 View
I run the simulation through NAMD and analyzed it by using VMD. I have chose the best position of protein-ligand docking. However, the RMSD graph of wild-type is higher compared to mutant. Is it...
18 November 2021 8,390 4 View
I tried 3 antibodies from different company nut none of them is working. I am retrieving the epitope with Citrate buffer pH 6.5 with heating 110 C for 10 min. I would appreciate if anyone share...
17 November 2021 8,454 0 View
Hello, I need to prepare the citric acid solution with a concentration of 0.2M, the citric acid powder will be used. How many grams of it should be resolved with water???
16 November 2021 9,128 8 View
Hello, we using the same amount of RNA for cDNA synthesis, and we are sure there are no hand mistakes for doing qPCR. using both TaqMan and SYBER green master mix, the qPCR instrumentt name is...
15 November 2021 3,691 5 View
Hi, I am synthesizing pentapeptide (GGGYK-Biotin) by FMOC SPPS using Rink amide resin. GGGY is a linker and K-biotin is a label. Unlike other fmoc amino acids, biotin-lysine did not get dissolve...
11 November 2021 1,129 4 View
AT2 cell infection or in general mouse lung cell infection via AAV needs certain serotype. There are many suggested but what works best? Than you!
09 November 2021 7,169 2 View
Hi everyone, I need a dialysis tube for my synthesis. There is one piece of dialysis tube (12400 MWCO) that has been stored at 4 C fridge in distilled water for 2 years. I was wondering if this...
08 November 2021 1,543 3 View
I have a question on the interpretation of my SDS PAGE gel. I know that I have protein degradation visible on my gel, but I am not sure how I can know which bands are degradation products of my...
30 October 2021 4,158 1 View
Hi, I am wondering if there is a correlation between colony size on a YPD plate and fitness. I am working with S. cerevisiae. Also, does the fitness calculated with the size of the colony also...
19 October 2021 1,283 4 View
I need to do a calibration line of 4-nonylphenol in methanol with a HPLC-MS. The main problem is the lowest detection limit that I obtained was very high (around 0,1 ppm), so is impossible to use...
18 October 2021 1,930 1 View
I am trying to download all the smiles of a similar structure of a particular compound from PubChem.
12 October 2021 6,051 4 View
Hi, I´m investigating the homo- and heterodimer formation of two proteins (A & B). For further results, I would like to perform experiments to understand where the homodimeric complexes (A-A,...
11 October 2021 5,150 2 View
Hello, I have ABTS that in the past I used to measure reductive activity. Now I want to measure oxidase activity. I was wondering if I could use the ABTS as a substrate for this experiment. I...
09 October 2021 2,942 8 View
Hello! I am looking for examples of industrial processes which are catalyzed by enzymes (either natural or recombinant). Particularly, I am interested in processes that end up with enzyme removal...
09 October 2021 1,633 6 View
Hi, I am doing enzyme kinetics of Gtpase and ATPase reaction. In calculating the the parameters I found by plotting the substrate against velocity the enzyme has more Vo values for ATP than GTP....
08 October 2021 3,824 7 View
Dear all, Does anybody know about this species?
06 October 2021 1,965 12 View
After ExonucleaseI digested a strand of 58bp and incubated at ssDNA,37 ℃ for 2 hours and inactivated 15min at 85 ℃, the negative value was still very high after fluorescence verification. It is...
05 October 2021 7,936 1 View
Does anyone have videos of the QIP Short Term Course on Advanced Ceramics and Composites for Multifunctional Applications (1-6 March 2021)? If anyone has the course videos of IIT Kanpur, please...
04 October 2021 5,702 1 View
I performed a fungal and enzymatic treatment in the lab and now I will do this pretreatment in the field. In my opinion if we add the fungi directly to the field there will be a great risk of...
03 October 2021 4,975 2 View
Hi all, So I performed SPPS using Wang resin. After incorporation of the first amino acid (Ser), I did Fmoc quantification and the yield was around 30%. So I re-incorporated Serine once more into...
29 September 2021 6,445 3 View
Hello, I would like to know which book or article you recommend me to consult the dry base composition of the fungi or specifically of the Aspergillus, since from this I have to formulate my "own"...
29 September 2021 3,973 7 View
Hello! It has been a little difficult to wrap my head around the process of this quantitative systematic review procedure. I used PRISMA to create a flow-chart of the 25 studies that met my...
25 September 2021 5,744 4 View
I'm trying to grow scenedesmus obliquus, chlorella vulgaris, scenedesmus sp and chlorella sp. On Monday I inoculated 4 mL of inoculum in 4 mL of BG11 medium (prepared as 1 mL of BG11 and 999 mL...
24 September 2021 1,909 4 View