Biological molecules that possess catalytic activity. They may occur naturally or be synthetically created. Enzymes are usually proteins, however... | Contact experts in Enzymes to get answers
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Questions related to Enzymes
Hello everyone, I've been encountering a recurring problem with the YES (Yeast Estrogen Screen) test, specifically related to the development of an intense red color after adding CPRG. I'm using...
08 February 2024 1,123 0 View
I am doing ADCC experiments with human sera, using virus-infected epithelial cells as targets and Jurkat Lucia NFAT CD16 cells (Invivogen) as effectors. In the experiment, I seed a 96-well plate...
06 February 2024 4,641 0 View
I prepared a chromium stock solution with a concentration of 1000 ppm. I used chromium metal powder with 99% purity, taking 1 g of the metal powder and dissolving it in 3 mL of HCl. Later, I added...
03 February 2024 2,341 6 View
I study diabetic tendinopathy and would like to know the reasons for choosing rat animal models over mice when it comes to metabolic syndrome. Your help and discussions are very valuable to me....
03 February 2024 1,822 3 View
Hello, I have nylon fabric dyed with Acid dye. I need to prove for part of my project, is it possible or not possible, the reaction between dye molecules absorbed in nylon fabric with water...
02 February 2024 1,651 4 View
Hi guys, I am performing MTT assays to charactherize tumor cells resistance to chemotherapy (I am comparing the treated cells vs. control (non treated) from different tumor cell lines). I have...
01 February 2024 2,596 4 View
Crosslinking on surfaces makes the enzyme reusable multiple times, I wish to know whether this is possible for a chemical reagent. Once fixed or coated on a surface, can the reagent be used...
30 January 2024 7,146 1 View
I have started to work with BV2 cells. First, I would like to condition the cells with DMEM 1X. The day after passing the cells, most had adhered to the flask surface; after another two days, most...
30 January 2024 4,431 0 View
Hi all, I have limited experience in proteomics and would like your opinion. I run proteomics analysis in three groups A: study group, B: first control of a disease with overlap with the study...
30 January 2024 666 2 View
Dear Colleagues Which the best type of regression analysis can be performed to test the affect the treatment on the single/multiple outcomes? Dependent Variable is continous such as thiskness of...
29 January 2024 8,920 5 View
I am curious whether the pKa of a charged side chain on the surface of a protein can be influenced by the charged state of nearby amino acids. My hypothesis suggests that, in comparing Figure 1A...
27 January 2024 7,360 3 View
I am trying to do a western blot of cytokines (iL-10, IL-6) of muscle tissue, using a strip of lysis buffer, 50 ug of proteins and blocking in 5% skimmed milk, however no signal appears, any tips?
25 January 2024 6,990 2 View
Colleagues, tell me which dye is optimal for staining RNA during gel electrophoresis? Which one do you use in your laboratory?
22 January 2024 6,340 4 View
I have conducted a mushroom cultivation trial for study purposes. I inoculated my substrate with oyster mushroom spawn about a month ago. Now, it has completed the incubation period. There is a...
22 January 2024 5,318 4 View
I changed the H-ESI needle on TSQ Quantis Mass Spectrometer and found the ion spray current turned down to zero. It was around 31 uA before the change. Does anyone know what caused this issue and...
19 January 2024 5,218 0 View
There are various hybrid devices of battery and supercapacitor, such as supercapattery and metal ion capacitor. The latter is best represented by the lithium ion capacitor (LIC) whose connection...
19 January 2024 7,249 1 View
Hi i have tried through literature search find out how "bendy" m13 phage is. and so fare i have come back with nothing. there are papers describing how much the phage can stretch but nothing...
18 January 2024 2,235 0 View
I am trying to generate topology file in GROMACS for an enzyme (PDB code =1HBN). But I encountered the following error. Fatal error: "The residues in the chain ALA2--ALA549 do not have a...
18 January 2024 5,065 2 View
Please help me. I conducted a cell proliferation experiment, continuously diluted threefold from 10000nM to 1.5nM, and processed the data using Prism. Why is it that the fitted IC50 value cannot...
24 December 2023 2,086 2 View
I was performing free energy calculation using g_mmpbsa tools. From the summary data i got the value of binding energy, but when i check the output file, it shows only data energy for the complex....
28 November 2023 4,589 3 View
Dear all; I'd like to inquire about the TL fading. Usually the TL fading decreases by passing the days. But based of the data that I got it, I found the fading is oscillated as in the attached...
18 November 2023 9,942 1 View
sodium acetate C2H3NaO2.3H2O
30 October 2023 9,174 1 View
We are going to use the AAV.rTH.PI.Cre.SV40 sold by Addgene to specifically manipulate dopamine neuron activity. https://www.addgene.org/107788/ We know rTH means rat tyrosine hydroxylase, and...
20 October 2023 7,911 0 View
I am trying to insert a gRNA into my lentiviral vector backbone (image is attached) at an AarI restriction site. I am unable to figure out how to design primers to add the restriction sites to my...
18 October 2023 4,447 0 View