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Questions related to Biochemistry
I am a new researcher working on the extraction of essential oils from specific plants, starting with their leaves. However, I have encountered an issue with my Clevenger apparatus. Despite...
02 June 2024 2,419 7 View
Good day! Please tell me, is it correct to compare two structures obtained using homology modelling on the SwissModel website and then compare them using RMSD in PyMOL?
19 May 2024 3,548 1 View
I am retiring, but still interested in academic work. How can I change the email to which you send notifications? Thanks.
13 May 2024 6,414 1 View
Can rats that were injected with collagenase NPs, show higher collagen deposition than their control untreated counterparts in histopathology examination!!!
12 May 2024 2,910 0 View
What o do when I lost ma password and want to change my mail adress as beein retired?
25 April 2024 8,612 1 View
Regional anesthesia techniques play a valuable role in pediatric surgery by providing effective analgesia, reducing the need for systemic opioids, and minimizing postoperative pain and side effects.
12 April 2024 8,996 1 View
Actually, the Zeta potential measurement instrument (Zeta Sizer, DLS method) needs the refractive index and absorbance values of the hydrogel beads. How can I measure these two parameter values...
29 March 2024 6,927 4 View
How to write hypothetical research proposal on climate change adaptation strategies adapted by farmers.
26 March 2024 3,961 2 View
When we started the experiment at first at 1ppm all the fishes died within 12 hours. repeated the experiments with lower concentrations starting from 0.05 ppm and found a very good response to the...
03 March 2024 3,076 8 View
Materials include SnCl2, SbCl3, and citric acid.
22 February 2024 4,438 0 View
Hi, I am interested in performing a reporter assay in organoids grown in matrigel. Is there a commercially available kit that would allow me to read both firefly and renilla luciferase...
07 February 2024 6,004 0 View
I have tried to achieve a separation of the nucleus and cytoplasm by using RLN buffer+ NP40 (0.2%), and then proceeding with GeneMATRIX universal RNA purification kit (the same used for total RNA...
05 February 2024 7,150 2 View
Hello, I would like to know which method to use to assess the effect of an electric field on tumor cells in culture. I would need to subject the live cells to variations in the intensity of the...
01 February 2024 8,820 5 View
I was hoping to find yield stress value using Carson or Herschel bulk key model.. I tried to fit viscosity vs shear rate plots to these models. Howeve, I can’t select these two model in our...
01 February 2024 6,295 0 View
I would be grateful if anyone could provide the answer to these question. I am using discovery hybrid rheometer HR-3. Thanks a lot
01 February 2024 4,377 0 View
Can someone tell me in detail about how to calculate the FIC index ? See attachment below:
24 January 2024 7,900 0 View
I ran an SRB toxicity test with Camptothecin and water as a positive and negative control for my tea samples. However, I tested my 2 samples separately, at different times. Thus, the tested values...
07 January 2024 2,724 0 View
Whenever I input my created mesh file in the “Tide Prediction of Heights” on Mike Zero, the longitude and latitude of the start and end points are not automatically detected. And as a result, I...
07 January 2024 2,613 4 View
Hi! Has anyone worked with negative-sense RNA viruses? I need to express recombinant proteins using a vector, so it will be necessary to start with ssRNA(-) to obtain cDNA for selecting the gene...
02 January 2024 9,414 3 View
What are the basic advantages and disadvantages of using both the methods? Which is more helpful?
23 December 2023 9,003 5 View
I am working on the design of the anaerobic baffled reactor for the treatment of dairy wastewater. I need assistance to determine the HRT and OLR for the reactor.
19 December 2023 1,166 3 View
I am log-transforming some environmental data for principal components analysis (PCA), in hopes of removing outliers. However, it did not seem successful for many parameters, such as TP, Salinity,...
21 November 2023 7,016 3 View
Hi all, I have been trying to detect several polypeptides by WB. And tricine-sds-page (16.5% or 20%) and glycine-sds page(20%) gel electrophoresis followed by Comassie blue staining showed no...
20 November 2023 7,420 3 View
Hi, To be able to Measure the Total Carbohydrate on my sample, I was planning on using the Total Carbohydrate Assay Kit (Sigma Cat#MAK104). The problem is that it includes a step in which I have...
14 November 2023 8,518 3 View