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Questions related to Biochemistry
Is there a tool that can output the most correlated/inversely co-related gene combinations from TCGA dataset? I mean WITHOUT AN INPUT GENE. Just give the highest R value combinations from TCGA...
14 July 2023 5,712 0 View
I would like to do an experiment blocking TNF to reduce neuroinflammation.
10 July 2023 8,098 3 View
The background of my Western Blots is very uneven. I use premade NUPAGE gels and PVDF membrane. For blocking I using intercept blocking buffer for 1 hour at room temperature. Primary antibody is...
03 July 2023 3,447 3 View
Hello all, My goal is to crystallize RNA-bound HIV-I Integrase. But once I mix equimolar amounts of RNA: protein (100uM), it immediately turns cloudy. Buffer of the protein is 20mM HEPES 7.5,...
23 June 2023 7,515 2 View
Aloevera /nanofiber/electrospinning
19 June 2023 5,992 0 View
hi everyone... i am working on bovine collagen protein but i am not getting proper bands in SDS PAGE. please give me some suggestion . thanks.
18 June 2023 6,185 2 View
Looking for Protocol of GRIESS reagent test on brain. many thanks
13 June 2023 7,768 1 View
Currently, i cultured a recombinant protein in E. coli BL21 with a variation in agitation speed. And in my result it showed that an increase in specific growth rate does not correspond to an...
12 June 2023 7,715 4 View
I am testing TCID50 of Influenza virus work seed batch with MDCK cell lines.The experimental process is as follows： 1.Put MDCK cells in 96 well plates at 16000cells/well in DMEM +10% FBS (100ul...
07 June 2023 1,804 1 View
I have decellularized the umbilical cord with detergent based methods, the problem is that I am unable to solubilize it with pepsin and HCl, kindly guide me with the exact protocol of...
02 June 2023 772 2 View
Can we use the Independent T-test when standard deviation is zero for one group, or go to non-parametric test? If some values have no variation and others with zero number, the calculated SD is...
05 May 2023 9,371 10 View
Hello, Have you ever extracted nuclei from fixed samples ? When I fix my HepG2 cells with 1% formaldehyde, I noticed that the fixation prevents cell lysis with regular nuclei isolation buffer,...
04 May 2023 502 5 View
i am cloning my gene of interest in vector pet32 and pgex4t. upon transformation i got colony on antibiotic containing LB Agar. also colony pcr and pcr with the plasmid extracted from the clone...
23 April 2023 1,111 4 View
Normmally you would go from pFastBac to DH10Bac or paceBac to Multibac. I currently have paceBac and DH10Bac and want to avoid ordering multibac if possible. Thanks!
21 April 2023 6,282 2 View
I have been working on B. subtilis transformation for a while, and despite many efforts and the use of different protocols I have not succeeded in doing it so far. I used the well known protocol...
17 April 2023 5,547 2 View
We have lost our melt calibration file for HRM analysis during software update and can't use precision melt analysis software for data analysis. We are using R programming based analysis for HRM....
28 March 2023 2,260 0 View
i have to use chloroform to measure the absorbance by Tecan plate reader but i can not do by using Polystyrene microplates (due to incompatibility of chloroform with Polystyrene). please guide me...
27 March 2023 2,053 0 View
Hi I am working with human bone marrow derived stem cell. I am trying to lyse and wash the RBCs in the sample. Any suggestions to what to use and if there is an exact protocol of how to do it?...
26 March 2023 6,388 1 View
I have tried to determine selenium in UV-Vis Spectrophotometer by using Methylene blue but it is very sensitive to time with in 30-60 sec i hv to measure and after that the color is vanished. so...
24 March 2023 9,874 7 View
i need to react chromium and selenium in hydrothermal conditions
22 March 2023 6,138 1 View
I've tried a transformation with a gene from a wild strain, however, it seens that this gene is toxic for my bacteria, I need information about gene toxicity when you try to modificate a wild...
02 March 2023 9,196 0 View
I've been trying to synthesize NH2-HA recently, but it failed. Can someone please tell me why and how I can improve it? The synthesis steps were as follows: I dissolved 500mg of HA in ddH2O and...
02 March 2023 2,766 5 View
Elution buffer composition: 50mM Tris-Hcl(pH9.0) + 10mM reduced glutathione + MilliQ water
01 March 2023 8,176 2 View
This was from a whole cell lysis protocol using 125uL of Y-PER and 2uLof PIC. I agitated the yeast cell pellet twice and then centrifuged the samples. I added 10uL of 2X loading buffer+BME with...
22 February 2023 9,457 1 View