This group is designed for the exchange of thoughts on Biochemistry | Contact experts in Biochemistry to get answers
2,093 views 2,496 posts
Questions related to Biochemistry
I ran an SRB toxicity test with Camptothecin and water as a positive and negative control for my tea samples. However, I tested my 2 samples separately, at different times. Thus, the tested values...
07 January 2024 2,683 0 View
Hi! Has anyone worked with negative-sense RNA viruses? I need to express recombinant proteins using a vector, so it will be necessary to start with ssRNA(-) to obtain cDNA for selecting the gene...
02 January 2024 9,371 3 View
What are the basic advantages and disadvantages of using both the methods? Which is more helpful?
23 December 2023 8,956 5 View
I am log-transforming some environmental data for principal components analysis (PCA), in hopes of removing outliers. However, it did not seem successful for many parameters, such as TP, Salinity,...
21 November 2023 6,974 3 View
Hi all, I have been trying to detect several polypeptides by WB. And tricine-sds-page (16.5% or 20%) and glycine-sds page(20%) gel electrophoresis followed by Comassie blue staining showed no...
20 November 2023 7,369 3 View
Hi, To be able to Measure the Total Carbohydrate on my sample, I was planning on using the Total Carbohydrate Assay Kit (Sigma Cat#MAK104). The problem is that it includes a step in which I have...
14 November 2023 8,476 3 View
I have been considering the number of MGB probes I can use in my multiplex real-time PCR. The conventional advice suggests using up to two MGB probes, with the third probe being 'normal' - without...
13 November 2023 388 2 View
I have to perform an amplicon sequencing analysis (metabarcoding) with the plant primer trnL intron g and h. For the sequencing I need to add 5´ tags to the primers. There is a methodology to add it?
09 November 2023 3,739 0 View
Greetings everyone, I am trying to dock a planar ligand with a receptor molecule using Autodock tool. I want to freeze the torsions of the ligand. So, I changed the number of active torsions to...
08 November 2023 7,592 3 View
Kindly explain
20 October 2023 9,424 4 View
I'm purifying the phi29 DNApolymerase, but the protein activity is always low and it's accompanied by very serious nucleic acid contamination. I once purified a batch of highly active protein, but...
19 October 2023 823 1 View
i am working on the determination of the synergism activity of the herbal and allopathic drug. firstly i tried to find out the zone of inhibition of individual drug having lower (25, 50, 100 ppm),...
13 October 2023 137 3 View
In their website they mentioned it's IF is 5.8. But in the JIF2022 report, I did not find. Is it because of its inclusion in the Emerging Source Citation Index? and because of not included in the...
08 October 2023 1,015 1 View
What is difference between double beam UV spectrophotometer and UV-Vis double beam spectrophotometer?
04 October 2023 5,631 4 View
Now I have Sodium hyaluronate solution from 0.1%to 1%. What range of RPM would be feasible to make sodium hyaluronate hydrogel by using a high speed centrifuge?
27 September 2023 6,110 0 View
Now I have sodium hyaluronate powder which is 75kda to kda, what solvent should I choose to make a 1% sodium hyaluronate solution. Is PBS solution or water suitable in this case?
25 September 2023 2,703 1 View
Hi please can anyone guide me on how to retrieve clonal complex (CC) from MLST sequence of seven house keeping genes of E. Coli, and also to do spartial distribution of the CC. Also, due to...
15 September 2023 5,204 1 View
dose titration , dose escalation
14 September 2023 1,211 1 View
My nanopore sequencing run has generated some ''unclassified reads''. Can anyone explain what causes them to be unclassified and how to avoid them in future?
14 September 2023 1,330 3 View
There are different methods to determine LD50 of chemicals and xenobiotics, these include Lorke's method, up and down method, Karber's method, Reed and Muench etc. But which of those is relatively...
02 September 2023 2,659 0 View
Does anybody have a reliable method of being able to measure increased microbial biomass during a ready biodegradation test (e.g. OECD 301F) between the beginning and the end of the test (or even...
29 August 2023 7,500 6 View
I have been through the various paper where mostly the works of Chan has been cited. Does anyone have the laboratory protocols in the determination of very labile, moderately labile, less labile...
24 August 2023 1,050 0 View
I am trying to extract DNA from skin swabs of Salamandra atra but the DNA concentrations I get are very, very low (like < 0,5 ng/µl). I am using the Qiagen QIAamp DNA Micro Kit in combination with...
23 August 2023 2,512 2 View
Hi all! I recently started performing enzymatic treatment on Nannochloropsis oceanica in order to disrupt the cell wall. Throughout the different extracellular components I want to analyze, there...
22 August 2023 6,087 1 View