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Questions related to Biochemistry
I am looking for an online Ph.D program of interdisciplinary Hermeneutics which is carried out by prominent figures of the field, and I would be happy if any one could suggest me such a program.
3 weeks ago 4,291 1 View
I am planning to conduct ROS (Reactive Oxygen Species) studies in animal sperm to investigate oxidative stress and its impact on sperm quality. For this purpose, which is the better option to use:...
4 weeks ago 9,480 2 View
Hello everyone, I am working with a cell population that requires fixation and permeabilization for its identification. At the same time, I would like to assess intracellular ROS/oxidative stress...
last month 4,930 2 View
Hi, I wanted to isolate proteins using trizol method. During performing the method, i was unable to dissolve the pellet in buffer comprising 0.3 M Gn-HCl, ethanol, 2.5% glycerol. However i...
07 August 2025 5,483 2 View
I have derivatized a biopolymer with a dye is that possible i can calculate the binding efficiency by their UV absorptin spectra ?
06 August 2025 9,320 4 View
Hi all, I'm trying to utilize the Perls Prussian Blue staining method to look at iron overload in fixed bone tissue (specifically the bone matrix, around osteocytes) and, so far, have had little...
29 July 2025 3,494 0 View
Hello, I am purifying two proteins separately, but want to isolate them as a complex only. The best way I could think was to have them sit on ice together after purification for an extended period...
26 June 2025 9,067 3 View
I am using a chloroform: methanol (2:1) solution as a lipid extractant for my insect cuticle sample (femur) mounted on UV glue. However, the method I am using is fairly rudimentary- I wet a small...
05 June 2025 6,286 3 View
Subject: Request to Update Affiliation Name Dear ResearchGate Support Team, I am writing to request a correction to our university’s name as it appears on your platform. Currently, it is listed...
03 June 2025 6,091 1 View
Hi all, We have SpectroNanoSTAR plate reader with double-orbital shaking up to 700rpm. We want to do growth curves for Saccharomyces cerevisiae and Saccharomyces uvarum. So far, we always see...
27 May 2025 9,564 3 View
Hi everyone, Most studies on nanoplate instability focus on mechanical loads like compression. But in real-world conditions, I imagine other, less obvious factors could also trigger buckling or...
15 May 2025 4,379 0 View
I want to detect the genes responsible for resistance of Klebsiella pnuemoniae, Klebsiella oxytoca, Enterobacter cloacae, and Pseudomonas aeruginosa resistance to streptomycin. I need the primer...
15 April 2025 3,455 3 View
If I have whole-genome resequencing data from a group of cattle along with their pedigree information, what methods can I use to train a model for predicting offspring genotypes based on parental...
14 April 2025 7,841 1 View
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14 April 2025 5,445 0 View
Hello. How should I proceed with atf-3 ifstaining? Hello. I was going to try with ATF-3 and NEUN in drg in the Oxaliplatin Cipn model. But atf-3 doesn't express in neun. And I also see several...
08 April 2025 1,565 0 View
I am involved in a study that involves collecting a fresh poop pellet from our rats at specific times. Does anyone know the best way to stimulate a rat to poop? We are working with both male and...
01 April 2025 4,999 0 View
Tissue used (dry legume seed powder): 15.7mg, 15.4mg, 15.9mg BSA concentration for standards (0.5, 1, 1.5, 2 μg/mL) 500μL 0.1M NaOH used as extraction buffer 20μL of protein extract and 200μL...
21 March 2025 7,099 1 View
I am doing subcellular fractionation of nucleus, cytosol, mitochondria. To verify equal loading of lysates what loading control can be used?
19 March 2025 3,292 3 View
Hi...I am trying to culture the MSCs in peripheral blood following many publications...but i end up in monocytes adhering to my flasks...i could not see mesenchymal morphology or highly...
14 March 2025 8,798 0 View
Can someone explain to me how to make a calculation with sTD2 program (new version of the old sTDA program) by using as imput a .molden file generated by Orca (via orca_2mkl sub-program which...
10 March 2025 4,258 0 View
Hello, I'm facing a conundrum and was hoping to get some advice. We have 12 cryo tubes (all replicates of the same clone) of HEK293T cells preserved in our -80 that have supposedly had a plasmid...
04 March 2025 8,228 1 View
Dear Dr, I am reaching out after reading your article titled "A graphene-based electrochemical sensor for sensitive detection of paracetamol" with great interest. I would like to ask you a few...
28 February 2025 6,635 2 View
Procedure to immobilize nanoparticles using PES membrane filters?
20 February 2025 751 0 View
What is the easiest and most economical way of measuring dopamine serotonin and noradrenaline for a research
13 February 2025 3,563 1 View