The study of the properties and action of enzymes. | Contact experts in Enzymology to get answers
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Questions related to Enzymology
I have recently started working on improvement of wheat flour for baking. I have come across a research which involved the usage of xylanase, cellulase, beta-glucanase and carbohydrases for...
11 November 2019 3,773 1 View
I am using KinTek software to do a kinetic fitting. I have determined kinetics of the formations of several species in the time course of 5min and established a model for the enzyme (just as an...
10 October 2019 3,982 4 View
I am searching the pGex or pET plazid of HDAC6, if he is possibly for somebody and would give for me, would be needed for my work. I would like to test the interaction between my labor's new...
06 June 2019 4,299 1 View
I’m working with a rather complicated ping pong, bi-bi double displacement reductase enzyme that oxidizes NADH and then reduces a substrate. Interestingly, the enzyme has intrinsic NADH oxidase...
09 January 2019 3,688 5 View
01 January 2019 8,224 5 View
Hello can any one help me to get this book its really important "Principles of enzymology for the food sciences / John R. Whitaker "
11 November 2018 950 1 View
I used NOBA, a chromogenic substrate for phospholipases, to measure (for 120 min) the absorbance (at 405nm) of PLA2 in crude venoms from different snake species. Later, I plot the absorbance...
10 October 2018 8,865 14 View
Dear all, When titrating a high affinity ligand I am getting Kd values that are very close to the total protein concentration in the system. My understanding on this matter is that, under such...
10 October 2018 3,072 16 View
03 October 2018 3,397 11 View
What's the DEPC water protocol for removing contamination from racks and other utensils in lab?
05 August 2018 7,247 7 View
Reactions are carried out in 50 mM Tris–HCl, pH 7.5, 5 mM MgCl2 and 100 mM NaCl using 2 µM cysteine desulfurase (Nfs) in presence or not of 2 µM SufE1. BSA 2 µM can be used as control. In addition...
03 May 2018 995 3 View
While checking the effect of inhibitors on protease enzyme it was found that iodoacetate instead of inhibiting the protease activity it has enhanced the protease activity. I searched alot but...
13 March 2018 6,597 3 View
Kynureninase is an interesting enzyme involved in many diseases such as schizophrenia and viral diseases, is these a synthetic ligand that can be used to compare the activity of certain...
19 January 2018 2,523 1 View
I want to interpolate the amount of product formed (as concentration or % of conversion) vs reaction time in a biocatalysis process. The fitting equation should have as (y) the amount of product...
16 January 2018 6,927 3 View
I'm working on enzyme activity of SufS for Iron-Sulfur Cluster. I have a problem of positive control, while testing my gene (Enzyme) that show no activity, the procedure is follow. Positive...
11 November 2017 5,682 4 View
Hi, I want to characterise by immunohistochemistry fibronectin, collagen I and III on a biologic matrix but the heat unmasking scrap my tissue. There for, I am in the need to a gentle and...
11 November 2017 7,805 3 View
02 November 2017 5,862 3 View
In the texts, it's often written, that the ligand has affinity towards a protein (with appropriate affinity constant). However, to me it always occurred that the protein binds the ligand and...
10 October 2017 7,189 0 View
Hi, I have a decrease of 1/3 to 1/2 in the gene expression of gluconeogenesis enzymes (Glucose-6-phosphatase, Fructose-1,6-bisphosphatase, Phosphoenolpyruvate carboxykinase and Pyruvate...
06 July 2017 8,056 5 View
Please colleagues, I need to know how to convert the measure of enzyme activities in plants (Ascorbate peroxidase and Glutathione reductase) from the SI unit of U/L to umol/L. I found that most...
06 June 2017 1,011 2 View
Enzymology, Molecular biology, Biophysics
23 March 2017 1,797 3 View
03 March 2017 7,999 3 View
I am trying to re-determine the Km of the enzyme I'm working with. For some reasons, I'm trying to do this using a coupled assay. I am having trouble with that, because the Km that I am finding is...
27 January 2017 6,748 2 View
Dear Altruist, I am running some QMMM jobs for finding the transition state and later on I want to do the IRC calculation if i can find the transition state.For me it is now a big problem to...
12 January 2017 2,860 1 View