Biological molecules that possess catalytic activity. They may occur naturally or be synthetically created. Enzymes are usually proteins, however... | Contact experts in Enzymes to get answers
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Questions related to Enzymes
Sigma lysing enzyme L1412 has been discontinued. Is there a known commercially available alternative to this ?
20 April 2021 4,689 4 View
I am measuring glucoamylase activity in commercial amylase (Spirizyme, Novozymes) based on the starch-iodine method, according to the method described by Oliveira et al. (2019): Flexible and...
20 April 2021 9,948 2 View
Is this furin enzyme specific for covid-19 or all other viral penetration cooperating enzyme?
19 April 2021 6,931 3 View
I want to know about the topics in bioinformatics that are being focused on recently.
16 April 2021 8,412 14 View
Hi, I have an enzymes which degrade polymers(plastics). It seems there is no way of measuring product concentration due to enzymes scissoring polymers randomly. In this case, heterogeneous...
15 April 2021 694 2 View
I used maxiprep to purifiy some plasmids and digested with a restriction enzyme afterwards. However, not only I see two bands I expected to be at 2.3-2.6 kb, I also saw a smear above the desired...
13 April 2021 1,863 3 View
Hi All! I am using ITC to study binding of lectin Concanavalin A to glycopolymer decorated with around 40 saccharide moieties (MW ~ 16 000 Da), which is multivalent-type ligand. Binding of...
13 April 2021 3,564 5 View
I have similar colony numbers for my self-ligation (EcoRV + Cla1) and my re-ligation (EcoRv+BamH1+Cla1 with two inserts). My No-ligation plate has no colonies at all. I wonder whats wrong with...
12 April 2021 7,808 4 View
I am currently conducting molecular cloning. I want to put a new restriction enzyme site in a given peptide sequence, and I want to preserve the peptide sequence as much as possible. More...
12 April 2021 1,098 3 View
Why it is a common practice to use S. cerevisiae alpha-glucosidase instead of the human enzyme, for in-vitro and in-silico/docking enzyme inhibition studies? Even the sequence of the yeast and...
12 April 2021 4,005 3 View
Can anybody help with delineating general tools for determining the stoichiometry of enzyme subunits in vitro and in vivo?
11 April 2021 5,473 3 View
I am trying to make a mutagenic library of a 2 kb insert in a 6kb yeast vector (pRS315) using Gibson assembly. I PCR out both my fragments, PCR purify them. Then I use cutsmart buffer to do Dpn1...
11 April 2021 4,158 0 View
Please let me know.
08 April 2021 6,842 6 View
Dear Expert Science community, I am interested to express a bacterial protein on the mammalian cell surface. As in mammalian cells, proteins are sorted by ER and golgi compartment. How should I...
08 April 2021 7,716 4 View
I have designed primers for vitamin D receptors using the IDT primer design tool. After that, I have checked its activity using the UCSC genome browser and Insilico PCR. The primers worked well...
08 April 2021 9,488 2 View
Hello, I'm trying to convert M to ng/ml, as one study used nM to calculate growth hormone levels and another used ng/ml. I looked up online the molecular weight of rat growth hormone and it says...
06 April 2021 5,704 2 View
Please suggest tools/software and also the labs with whom I can collaborate and explore this aspect of the project.
05 April 2021 6,270 1 View
In this article on Safed Musli: Clinical evaluation of root tubers of Shweta Musali (Chlorop... , the authors write "Statistically significant improvement was observed in serum testosterone in...
05 April 2021 3,356 4 View
Hello, I would appreciate someone's input on this question. In an article on ginger, which I have uploaded, testosterone levels are reported to test for the efficacy of ginger. They report...
05 April 2021 1,399 1 View
Hello, There's an article on Ashwagandha at: A Randomized, Double-Blind, Placebo-Controlled, Crossover St... It's a crossover study, switching between ashwaganda and placebo. It seems that for...
04 April 2021 5,779 2 View
I want to use KX2-391 (tirbanibulin) to inhibit Src in cultured fibroblasts. Is it known whether treatment with this compound results in decreased or increased phosphorylation of Src? I would like...
02 April 2021 8,429 0 View
Hi, In an enzyme kinetic experiment, my result showed a consistent decrease in Vmax by increasing magnesium but Km fluctuated. Till 40mM Magnesium, Km decreases, but it was increases at 60mM...
30 March 2021 3,623 16 View
I recently bought lactate oxidase from abroad and received it after 3 months. How can I check for enzyme activity? I know that for some enzymes such as glucose oxidase there are tests but is there...
30 March 2021 3,853 9 View
I have immobilized an enzyme on the immobilization resin Dowex. How can I determine the number of enzyme units present in the powder form. Is it possible that enzyme assay is performed with powder...
30 March 2021 2,495 2 View