Biological molecules that possess catalytic activity. They may occur naturally or be synthetically created. Enzymes are usually proteins, however... | Contact experts in Enzymes to get answers
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Questions related to Enzymes
What is the concentration of Clavulanate inhibitor that I should use in assay to obtain full inhibition of B-lactamase activity?
16 June 2023 2,779 1 View
Currently I am working on beta-lactamase enzyme. Proteins are well expressed and purified (plasmid pET28a)but not getting result of MIC in BL21/Top10 E.coli host cells while treating with...
14 June 2023 8,969 4 View
I am interested to know if there is a difference in the bacterial biofilm compositions between: 1. cool and warmer seas 2. on different surfaces Is anyone here investigating this and would like...
13 June 2023 3,016 2 View
I am working with bacterial lipase crude enzyme. Whether it should be stored in -20C Or -80C before and after lyophilization to retain its activity? Thanks in advance
13 June 2023 5,714 2 View
How do environmental conditions affect plants and how can the environmental factors and plant factors affect the photosynthesis?
10 June 2023 1,950 1 View
Fabricating the SMS structure, as mentioned in numerous SMS-related journals, is often described as an effortless process involving the cascading of a short-length multimode fiber between two...
05 June 2023 1,790 0 View
I am working with a panel data set of purchases over week. Therefore, a poission distrubition is suitable. When there is overdispersion, a negative binomal regression is adviced. I am combing...
02 June 2023 9,445 3 View
Hello everyone, I'm trying to measure the porosity of keratin/alginate hydrogel. I found high value (around 105%-110%) when I try to measure it using 95% ethanol. I have ever tried to use water to...
01 June 2023 3,242 1 View
Hi, I am using Gaussian 09w for my DFT studies. Currently, i am doing DFT calculation using LANL2DZ basis set for my copper(II) and zinc(II) complexes while, 6-31G+dp basis set for my ligand. The...
01 June 2023 2,930 2 View
Dear ResearchGate members, I would like to know if polymers such as Polyvinyl alcohol, Polyvinyl pyrrolidone, Chitosan, Carboxymethyl cellulose, etc. (with a molecular weight less than 40 kDa)...
29 May 2023 3,928 4 View
Is there a new access link for it?
28 May 2023 4,713 0 View
We'been preparing B5 media for plant cell culture (MS + Gamborg vit+ 3% sucrose) with and without agar, adjusting with KOH to pH 5.7. We noticed that the pH changes after autoclaving, around 0.7...
25 May 2023 764 2 View
We are trying to do the coupled cluster calculation in gaussian several time, but the jobs are terminated without giving any specific reason.
25 May 2023 6,029 4 View
I want to know that what kinds of chemical could be found in the plant which could be used as substarte for citric acid fermentation.
25 May 2023 1,823 0 View
I am using some restriction enzymes, and according to the protocol, the amount of enzyme should not exceed 10% of the total reaction volume to avoid star activity. This activity is due to the high...
24 May 2023 4,105 2 View
Recently I am stuggling to improve the kinetics of an artifical enzyme. I expressed this enzyme in E.coli and then test it's kinetics properties. I noticed that if I pick single conies for...
23 May 2023 2,273 6 View
I have been trying to determine the p-anisidine value in heated sunflower oil according to the AOCS method Cd 18-90. However, when I add the p-anisidine reagent to the homogeneous oil solution in...
23 May 2023 9,635 0 View
Hello, I am searching for a protocol to create a immobilized melibiose column to purify anti-alpha-Gal antibodies from human serum. the crosslinked guar gum column described here Preparation and...
22 May 2023 753 0 View
We only tried lipo3000 and lipo6000, but the transfection efficiency was not good.
22 May 2023 2,602 2 View
Kindly share any recent research paper regarding the same?
20 May 2023 5,700 3 View
Dear Colleagues, I have carried out research to find out the nature of stalking, its socio-phonological impacts on victims, and strategies used by victims to cope with stalking. However, the...
16 May 2023 3,748 8 View
I wrote the article Israel’s Targeted Killings of Terrorists: A Life-Saving Tactic That Is Legal, Effective, Precise, and Just. You can not claim I didn't. There is only one Alex Grobman, who is...
09 May 2023 5,777 2 View
I am considering drying sediment samples from mangroves at room temperature to obtain marine actinomycetes. Is this method allowed?
07 May 2023 3,229 0 View
I am working on cerebral arteries (middle and posterior mainly), isolated from mice for pressure myography experiments where I observed fluctuation of cerebral artery often. I am using PSS with...
27 April 2023 295 0 View