Biological molecules that possess catalytic activity. They may occur naturally or be synthetically created. Enzymes are usually proteins, however... | Contact experts in Enzymes to get answers
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Questions related to Enzymes
I am using 96-well polypropylene plates for UV-Vis absorbance measurements. I would like to clean and reuse them, but I want to ensure that the cleaning process does not leave residues or affect...
01 April 2025 1,076 2 View
Now : - (1) My name Christian Mira appears in two Research Gate pages : (1a) https://www.researchgate.net/profile/Christian-Mira/research with 1,762Research Interest Score, 3,466 Citations,...
01 April 2025 2,886 1 View
We are suspecting that it is isolate from tg1 but eventhough i have transformed that to dh5 alpha and have done isolation
28 March 2025 1,465 1 View
Phishing email header datasets contain various features that help identify phishing attempts, such as the sender’s email address, recipient’s address, subject line, and the email's routing...
27 March 2025 5,604 4 View
Does anybody have a tried and true method for generating dendritic cells from mouse bone marrow? I have tried about half a dozen protocols and I keep getting macrophages! And small cell counts as...
26 March 2025 3,689 1 View
Hi ResearchGate community, I’m encountering a problem purifying a highly hydrophilic peptide (2.5 kDa, ~10% hydrophobicity) using a preparative C18 column. Despite using a 0–30% (also tried:...
25 March 2025 2,054 8 View
Some of my publications are qualifies as ”books” but in reality they are chapters of books. How can I change this?
24 March 2025 8,213 1 View
Dionisia
23 March 2025 9,267 1 View
I try to install the 2022 version on the Linux system, but because the versions of various packages on my computer are relatively new, I can't install them. I can only find the Windows version on...
23 March 2025 5,260 1 View
I recently ran a gel electrophoresis experiment, but I encountered issues with my DNA ladder not separating properly and my bands appearing wavy and distorted. Here are the details of my...
21 March 2025 6,746 3 View
i get stucked in my project and i need to coonstruct my PKN
21 March 2025 2,177 0 View
I want to determine the MICs of these two pesticides on bacteria from the eskapee group. But I can't find a good protocol for this. I've tried DMSO and methanol but the latter are known to inhibit...
18 March 2025 5,624 0 View
What specifications of ion-exchange chromatography (manufacturer? particle size? resin type?) should I use to purify monosaccharides (e.g., glucose, fructose) and disaccharides from mixtures to a...
17 March 2025 2,953 1 View
Some of my samples lost low molecular weight proteins after migration and transfer. It happens around 20-40KDA. I used : -Ripa buffer with SDS, protease and phosphatase inhibitor...
13 March 2025 1,405 3 View
Emotion analysis using deep learning is evolving rapidly with advancements in NLP, multimodal learning, and explainable AI. Researchers are exploring various approaches, including...
12 March 2025 4,069 1 View
KHAN, R., 1987. Pharmaco-anatomical evolution of bark and leaf of some arborescrnt Moracrae. Upublished Ph.D. Thesis of Aligarh Muslim University, Aligarh, India . Thanks
12 March 2025 7,672 3 View
LLMs are evolving to process text, images, video, and audio simultaneously. This question explores how they integrate different data types, focusing on alignment issues, model architecture...
11 March 2025 1,856 0 View
RAG models combine retrieval-based search with generative AI, but efficiency depends on how well relevant information is retrieved. This question examines vector search, knowledge graph...
11 March 2025 803 0 View
I need to do cytotoxicity study of some anti-cancer drugs on normal cells HEK293T. But the cells are detaching from the well after treatmet with MTT. How to solve this problem?
10 March 2025 3,885 1 View
How can I write a discussion of a research paper?
08 March 2025 701 1 View
I’m researching the performance of resin-based vs. cement-based permeable pavements and would love to hear your insights. Resin-based permeable pavements, such as PurePave (purepave.com), offer...
07 March 2025 1,481 4 View
Voters Perception on Nonverbal Gestures
06 March 2025 6,571 0 View
I am working on simulating a Graphene Field-Effect Transistor (GFET) in TCAD Sentaurus. Since the tool does not have predefined graphene parameters, I plan to model graphene based on polysilicon...
05 March 2025 2,486 0 View
As a consequence of globalization, every nation nowadays is exerting effort on calling for fostering values especially ethical ones and cultivating them in the students especially young learners....
02 March 2025 8,485 5 View