Biological molecules that possess catalytic activity. They may occur naturally or be synthetically created. Enzymes are usually proteins, however... | Contact experts in Enzymes to get answers
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Questions related to Enzymes
Hellow, I am planning to do enzyme kinetics study. In preparing, I have a problem in preparation of calibration standards as pmol unit. For example,=> Metabolite standard (Purity = 100%,...
28 May 2021 1,794 1 View
Dear Researchers, I am cultivating Vibrio Phages in TSB media (20PPT salt). First, when i treat with DNase and RNase it is unable to remove complete 100% DNA (Host DNA). Any suggestion, how to...
27 May 2021 4,652 0 View
I always got IC50 of small molecule TKIs from cancer cells viability assay(MTT, Alama blue) 50 – 200 folds higher than IC50 from enzyme inhibition assay. What factors affect them?
26 May 2021 8,038 3 View
Hello, For a while, I have been conflicted on what could be the 'right' way to analyse my data. I am doing calcium imaging in individual cells using fluorescence microscopy. A stimulus is added,...
25 May 2021 732 2 View
We know that GST has a large protein size, 225.5 KDa. When other tags are available, say 6X His tag, then why is a tag with such a huge molecular weight used which might interfere with downstream...
24 May 2021 8,894 7 View
My ligand is a small gold nanocluster built from a unit cell. Thus it must be rigid. I added needed constants for gold to the parameter file in the autodock and put the number of active torsions...
23 May 2021 7,233 7 View
I'm trying to dock an enzyme with its ligand which is an amino acid. The software doesn't recognize the amino acid as a ligand but as a protein. What can I do to solve this? I'm using...
22 May 2021 1,689 4 View
Hello! I'm looking for an enzyme that is able to transform unsaturated fatty acids into saturated fatty acids. Kinda like what enoyl-coa-hydratase does in B-oxidation, but anything remotely...
22 May 2021 9,302 3 View
Hi, I am curious if there are differences between a pdms microfluidic device coated with and without Poly-D-lysine coating? I am adding fibrin gel in this device and I wonder if the gel is more...
21 May 2021 9,007 0 View
I have prepared a standard curve for Pyruvate for ALT and AST estimation in serum using manual method. I need to convert Pyruvate (uM) to transaminase activity in U/L. I referred to literature and...
21 May 2021 8,329 4 View
we have determined mode of inhibition by plotting 1/V against 1/[s] in lineweaver-burk plot. Can we use the initial rate data to determine the order of the reaction with respect to inhibition and...
16 May 2021 5,304 6 View
I'm working in the field of plants, isolation, and structure determination of biologically active natural compounds. I need NMR of proton, carbon-13, 2D,... Any suggestions in this regard; free...
13 May 2021 2,589 5 View
I want to purify my inclusion body protein by using AKTA. pI of the protein is 9.4. Can Someone help me which column should I select ? CM sepharose or SP? Please suggest me the procedure. Thank you.
13 May 2021 4,230 0 View
I am thinking of implementing an Atomic Force Microscope for nanoindentation-assisted nanolithography experiments and I don´t know if photoresist films are generally adhesive to glass substrates....
11 May 2021 5,327 6 View
Natural fibers are usually obtained by retting of stems of bast fiber yielding plants and then beating them manually or mechanically to separate the fibers. I would like to know if there is any...
09 May 2021 3,453 3 View
if after cutting i realize that the digestion (and heat inactivation if enzymes) isn't complete, can i again add the same enzyme and continue the digestion reaction?
08 May 2021 7,263 3 View
I am looking for synthesizing cDNA from total RNA, isolated from wheat leaf. I have GoScipt Reverse Transcription Kit. After some searching, I have found that this MMLV reverse transcriptase does...
05 May 2021 1,209 2 View
Hi all! I have a lysozyme that i mistakenly stored at 4degC instead of -20degC. I would like to ask if it will still work in DNA extraction? The said enzyme is lyophilized. Hope you may help me...
03 May 2021 9,939 10 View
Hi there! This week I extracted DNA from leaves using the protocol for HMW DNA, which employs Carlson lysis buffer + Genomic Tip columns (Qiagen). I quantified it with Qubit (920 ng/ul) and...
29 April 2021 5,097 3 View
I have read many papers but I want something to start with so that can gain some experience for my future projects. I am a Master's student and want some hands-on dry lab experience.
28 April 2021 651 4 View
Hello, My raw materials are oil palm fronds (OPF) and empty fruit bunch (EFB), in which I will extract lignin. So I am using NMR spectroscopy to amalyze the structure of OPF, EFB and...
23 April 2021 9,855 0 View
I am trying to optimize the Enzchek Phosphate Assay kit (Thermo Fischer Scientific) for a reaction volume of 100uL, while the kit has been designed to work in 1mL. I calculated the reagents...
22 April 2021 1,899 10 View
The allosteric mechanism of the enzyme is difficult to get in the nanoseconds range in molecular dynamics simulation. In my enzyme, the allosteric regulator activates the enzyme throughout a large...
22 April 2021 3,966 7 View
Hello. Im currently doing protease assay according to the method provided on SigmaAldrich website (https://www.sigmaaldrich.com/technical-documents/protocols/biology/protease-activity-assay.html)....
22 April 2021 5,738 3 View