Biological molecules that possess catalytic activity. They may occur naturally or be synthetically created. Enzymes are usually proteins, however... | Contact experts in Enzymes to get answers
1,229 views 2,728 posts
Questions related to Enzymes
I've found dozens of databases with enzymes and catalytic peptides but none of them seem to have a clearly stated catalytic efficiency. Even a site with metadata or any sort of machine readable,...
13 April 2022 8,017 0 View
I have been asked by a reviewer to give Goodness of fits to some curves in a manuscript but I am uncertain what is the most correct way to do it. The data is from non-linear regression of...
13 April 2022 8,255 5 View
I am about to calculate alpha-amylase activity for some fungal samples. I followed the protocol of Sigma-Aldrich...
12 April 2022 3,737 9 View
I noticed in a particular result, the higher the concentration, the percentage inhibition was equally going higher. But there is an issue, when the IC50 was calculated using Linear regression,...
12 April 2022 5,134 3 View
I am trying to purify an hydrophobic recombinant protein containing a His-Tag. Is there any suggestion about lysis and wash buffers? To eluate I am using imidazole.
07 April 2022 5,744 4 View
Hello, I'm an undergraduate assisting in analyzing neuronal images. Using Imaris, I've been able to automatically count spots in the 2D image, but each spot is the same size. Is there a way to...
06 April 2022 1,688 1 View
Hello! I noticed that my HeLa parental cell line has these strange vesicles in a perinuclear localization (arrows). Regardless the vesicles, the cells look and behave fine and they are not...
30 March 2022 4,797 0 View
I have read that some article distinguished between proliferation and migration in Wound Healing Test. But does HaCat (keratinocyte cells) need the differentiation as it does not contain any...
28 March 2022 9,686 0 View
I want to give osmotic stress to in vitro plants using PEG 6000. can anyone suggest protocol for preparing 20% PEG 6000 solution in liquid MS media? Thanks
23 March 2022 4,600 0 View
Hi everyone I want to test the electrocatalytic activity (Overall potential at 10 mA/cm2 and long-term stability) of my Bifunctional electrodes in a two-electrode system which means that I have...
19 March 2022 7,545 5 View
Good morning everyone We did mating assays using Carolina´s pGreen plasmid and were successful. However, the reviewer is asking us for the replicon of this plasmid and this information is not...
16 March 2022 5,677 4 View
Our lab is planning to use the Dahlqvist assay of disaccharidases (Dahlqvist A. Scand J Clin Lab Invest 1984). However, an important ingredient - Glox Novum from Kabi Diagnostica AB, containing...
06 March 2022 8,539 0 View
Dear colleague, I am doing MC3T3-E1 differentiation. Previously I grow the cell in growth medium (I used DMEM + 10% FBS + 1% PS). When they reach ~ 70% confluency, I changed the medium to...
24 February 2022 5,839 0 View
I'm not planning to perform any experiments using the iTC 200. So I was wondering whether to keep the sample cell empty or full ....
21 February 2022 3,437 5 View
I'm a PhD student in polymer physics particularly on photophysics of conjugated polymers. While reviewing literature I come across an article by Shu Hu and coworkers on 'Effect of Thermal...
21 February 2022 2,895 2 View
Hello everyone, I am trying to evaluate by western blotting proteins secreted by cells to the culture medium (in cell cultures). I found a precipitation protocol with TCA/Acetone...
21 February 2022 9,032 9 View
Can anyone tell me the relative permittivity/dielectric constant variation (any algebraic expression or graph would suffice) in ZnxCd1-xTe (Zinc Cadmium Telluride)?
14 February 2022 3,155 0 View
I have taken the growth factor protein at concentration 0.03 mM in sample cell and 0.09 mM receptor in the syringe. The binding affinity of this protein-receptor is already known with 1:2 ratio. I...
13 February 2022 2,455 3 View
I'm trying to measure DNA damage levels in human PBMCs using the Abcam anti gamma-H2AX phospho-serine 139 antibody (ab11174). All the images I've seen of gamma-H2AX westerns have a single band,...
13 February 2022 4,658 4 View
I am asking about the preparation of protease enzymes by concentration similar to the produced by these bacterial strains
08 February 2022 5,305 2 View
Dear All, I need to remove MEGA 10 detergent from solution by dialysis by using Dialysis-Cassette but I need to choose what is the preferable MWCO that i have to use? My solution contain 30 nm...
07 February 2022 7,718 1 View
Hello, researchers can you provide some of your research about total starch or sugar content of any with regards to different types of cereal or grain plants. Thank you!
04 February 2022 747 4 View
I’ve been told: NEVER thaw viral stocks in a 37 degree water bath, always just thaw on ice RSV is too unstable to thaw that slowly it needs to be quickly (less than 30s) swished in a 37 degree...
26 January 2022 7,461 0 View
My current HPLC protocol parameters is: Poroshell 120 EC-18 0.8 (Decrease to 0.5 mL/min between 3.5 and 5 min) Acetonitrile (A) Water + 0.1% formic acid (B) Start: 5% A...
21 January 2022 2,323 3 View