Biological molecules that possess catalytic activity. They may occur naturally or be synthetically created. Enzymes are usually proteins, however... | Contact experts in Enzymes to get answers
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Questions related to Enzymes
NdeI restriction site was added in forward primer and HindIII was added in reverse primer size of band is looking near 500 bp but size of is less than 15 kda but expected size should be 17.7kda.
16 April 2024 202 5 View
With increasing sorbent dose in an adsorption test for heavy metal removal , there were various shade of decolourization in metal solution after adsorption observed. Does this colour change have...
14 April 2024 3,240 1 View
How does adding extra carbon dioxide to the atmosphere cause global warming and relationship between carbon dioxide and the greenhouse effect?
14 April 2024 6,848 3 View
Those familiar with that greenwashing is and the consequences of greenwashing should be able to see whether the answer to the question is short yes and why or a short no and why no without...
13 April 2024 5,717 4 View
I used the Intact Genomics FastAmp® Plant Direct PCR kit and when seeding the gel with the PCR product there was a lot of DTT smell. After running the gel, partially degraded dna was observed,...
11 April 2024 2,123 2 View
Hi, I am a fresh master's student who just started to study Idiopathic Pulmonary fibrosis(IPF), and I am here to ask about how to freeze lungs with LN2 when I harvested lungs from mice because...
04 April 2024 2,191 1 View
I'm doing my experiments with passage number of 67. Is that good to do with this high passage number?
04 April 2024 5,065 3 View
Hello, I want to know, is novel gene identification possible by whole metagenome sequencing dataset. When annotation of genes is done, we get various hits. Some of the predicted genes get...
03 April 2024 3,159 0 View
I am trouble shooting a malachite green ATPase assay using a kit from Sigma (MAK307) for a cytosolic protein that converts ATP --> ADP + Pi and am wondering what cheap + commercially available...
31 March 2024 9,634 1 View
I am trying to determine the cost of setting up MaxOne System in order to record and stimulate organoid. How much does it cost ? Are there any more cost effective alternative ?
31 March 2024 2,667 1 View
Using a questionnaire, I analyse the affective well-being after three different dance lessons at three points in time (before, during, after) in the same subjects. Background: the social...
28 March 2024 1,128 4 View
Previously I have cloned estA gene in pET28b with restriction sites EcoRI and BamHI and stored it in -80˚C. I also proceeded with site directed mutagenesis (SDM) after confirmation of the cloning...
28 March 2024 8,575 5 View
I have the PCR products of two bacterial genes (gyrA=1024 bp and rpoB=808 bp) and I want to know how many microliters I should add in the agarose gel?.
28 March 2024 2,780 7 View
I am working on a multiplex immunoflurescence brain tissue slides
27 March 2024 1,422 2 View
In real time PCR we dont have CT or ct35...
25 March 2024 8,099 4 View
I am working with an allergen and i am working using PCR, the result that offers the kit is copies DNA, although i need to give a result in mg/kg. Is any possible way? Thank you in advance,...
25 March 2024 5,610 3 View
I am working on expression and purification of one cytoplasmic protein with His tag, in E coli Bl21(DE3) host cell. Here is SDS picture. Line 2 and 9 are cell lysate, 3,4,7,8 wash steps and 5,6...
21 March 2024 6,426 5 View
I have a series (c. 400) of point-counts of breeding birds, taken in the same localities in 1992-1995 and 2022-2023 periods. My aim is to obtain spatial information from the comparison between...
21 March 2024 7,753 1 View
I'm studying the effect of the pH on the adsorption and I noticed that the intensity of the color changes when the pH increase ( from intense to slightly clear). note thar the maximum wavelenght...
19 March 2024 8,989 0 View
Would it be appropriate to compare this value with the IC50 value obtained from the MTT assay? How can I calculate it?
19 March 2024 8,320 1 View
I have been studying about Vagus nerve and I want to check the activity of the Vagus nerve in my research. Could you advice me about it?
18 March 2024 8,122 1 View
Is it necessary to quantify each standard with its specific compound, or can I use a single standard to quantify two different compounds with the same chemical structure, for example?
15 March 2024 4,940 2 View
How to download Colon cancer (COAD) gene expression data which includes tumor tissue samples and corresponding control tissue samples from TCGA(https://portal.gdc.cancer.gov/repository). Any...
15 March 2024 7,714 1 View
I am working on T cell receptor repertoire analysis obtained from RNA seq data. I am trying to understand the CDR3 length and amino acid usage in my data set, where I have variable lengths of...
13 March 2024 2,978 0 View