Hello, I would like to analyze quantitative PCR (qPCR) data using R rather than proprietary softwares. I can see there are several packages out there, but mostly they perform relative quantification, while none provide a quantitative cycle (Cq), or crossing over cycle. Is there a package that can provide a Cq for a given threshold level?

I can fit a model on qPCR data using for instance the package qpcR (https://cran.r-project.org/web/packages/qpcR/index.html).

The package allows the determination of a take-over point, but this is the cycle where the data start moving into the exponential phase: However, this level is too low; the Cq would probably be way higher on the y-axis, and there are instances where I would like to use a pre-fixed threshold value.

Is there a better package?

Thank you