A forum to address questions regarding enzyme-linked immunosorbent assay, most commonly known as ELISA. | Contact experts in ELISA to get answers
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Questions related to ELISA
If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis
03 March 2021 8,063 3 View
I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.
01 March 2021 6,737 3 View
I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.
01 March 2021 6,176 2 View
We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...
01 March 2021 1,454 1 View
I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...
01 March 2021 7,639 3 View
Hi all, I’ve made a custom antibody detection ELISA with a Corning Co-Star plate. Coated with antigen, followed by blocking and drying. Ran the standards on the plate and got good recovery (+/-...
25 February 2021 1,599 3 View
Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...
24 February 2021 7,680 1 View
I had control cells and performer ELISA analyses group (time 0). I stimulated cells with substance A and B and cultivated for 1, 2 and 3 hours. On each cells I performed ELISA. I have results...
18 February 2021 9,137 4 View
Hello, I am planning to perform an ELISA-based assay to quantify the percentage of genomic methylation (5-methylcytosine) in human embryonic stem cells. I've been searching for some kits and I...
17 February 2021 8,539 3 View
Hello, I am trying to know tomato virus titer/concentration in samples and I will perform serial dilution of the positive control. I wanted to ask How to estimate/know plant virus concentration to...
14 February 2021 6,706 4 View
Hello, i am writing a theoretical research proposal for one of my university assignments regarding Covid-19. The brief is pretty relaxed and i have chosen to conduct a study on how the...
12 February 2021 1,084 5 View
I am using indirect ELISA to measure the antibody response against Aeromonas salmonicida in Atlantic salmon. I am using 3 dilutions of serum 1:50, 1:200, and 1:400. The OD450 nm values measured at...
09 February 2021 9,218 3 View
Hi, Hope you can help. Currently been running some sandwich ELISA assays where I coat with anti-His, block and then capture His-tag protein and then sera and then anti-bovine monoclonal...
08 February 2021 7,224 1 View
Hello, In my sandwich-type ELISA which is testing for small molecule inhibition of a protein-protein interaction, it seems that DMSO percentage and dilution protocol are important. I...
01 February 2021 7,029 1 View
Hello, I am conducting a sandwich-type ELISA. It is to see if small molecules can interfere with a protein-protein interaction. As this interaction would occur in the human body, may I heat...
01 February 2021 4,468 3 View
I am trying to find axillary and mandibular lymph nodes in naive mice, which is turning out to be more difficult than imagined. Therefore, I would like to know if there are some possibilities of...
28 January 2021 9,805 3 View
Hi all, I am trying to establish an ELISA method for the quantification of a recombinant protein injected into mice. In the meantime I am trying to make calibration curves with the purified...
28 January 2021 8,911 3 View
I am using Elabscience sandwich ELISA kit (detection range 0.31-20ng/ml), I am using supernatant saliva samples. I am using 4 plates and plotting the standard curve for every plate. In one of the...
28 January 2021 9,900 1 View
I'm building an ELISA from an antibody pair kit (R&D Duoset DY291) and it seems I can't use BSA in case there's any bovine IGF1 (100% similarity to human form) present so it suggests I use...
20 January 2021 7,988 4 View
We'd like to use this kit for brain tissue samples (rat). When do we stop the reaction, 30, 60, and 120 minutes? We don't like to test at all three stop points, because we've too many samples.
18 January 2021 6,852 2 View
Has anyone used Human p-cresol ELISA? Our laboratory has limited access to GC-MS and HPLC instruments and is looking for alternative assays to determine p-cresol in serum. Anyone with an...
18 January 2021 4,898 7 View
Anti-FLAG ELISA can be used but the problem is that we do not have an antibody against our protein. Any suggestion will be appreciated. Thank you
12 January 2021 8,556 1 View
I want to evaluate the biodistribution of an elastin-based nanoparticle drug in tumor, heart, liver, and kidney for the first time. Can anyone give me any idea about how to prepare my tissue...
05 January 2021 3,805 3 View
Dear all, I have a question regarding the most appropriate fit of a standard curve in an enzyme-linked lectin assay set-up to quantify the amount of mucin secretion in conjunctival explant...
04 January 2021 1,221 6 View