I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples be compatible with ELISA?
Most likely not. Most ELISA are prepared by immobilizing antibodies and blocking reagents via hydrophobic interactions on the plate surface. SDS at this concentration could denature the capturing antibody and also wash away the layer of protein and blocking reagent altogether. How about asking the manufacturer of your ELISA?
Hüseyin Besir thank you very much for your reply.
I agree with what you say. However, I would like to do an indirect ELISA, therefore most of the SDS would be washed off by the time antibodies are added to the plate. What do you think would happen in this case?
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