A forum to address questions regarding enzyme-linked immunosorbent assay, most commonly known as ELISA. | Contact experts in ELISA to get answers
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Questions related to ELISA
Does anyone can help with providing a protocol for homogenization of rat brain tissue for ELISA kit? I would appreciate any knowledge about lysis buffer and stages in the process.
05 May 2019 3,813 3 View
Hi all. I'm looking to calculate the inter assay precision between two assay plates, plates of which have a 1:2 serial dilution from wells A-H across all 12 columns. How would I go about...
05 May 2019 8,491 1 View
I read the carb-bicarb pH 9.6 or plain old PBS (pH7.2) are used by different companies.
05 May 2019 4,875 7 View
I performed assays in microplates for the first time to determine enzymatic activity, but I made a mistake with the reading. The recommended wavelenght for the assay is 550 nm. I used an ELISA...
05 May 2019 2,667 13 View
Hello, We measured the level of pge2 in the culture supernatant of rat cells using a competitive elisa kit. the problem is that we got values out of range, which means that the rate of pge2 was...
05 May 2019 4,022 2 View
Hi, I want to see if my mice have gotten infected, is it possible to coat an ELISA plate with IAV broken up by sonication? Thank you!
05 May 2019 5,208 3 View
I have problem to detect different cytokines in supernatant after the treatment of cells with LPS or TNF alpha by ELISA. Can you suggest me the way for concentration cytokines in cell supernatant...
05 May 2019 7,829 8 View
02 May 2019 6,306 8 View
I produced a recombinant chimeric protein from CHO cells and purified it using size-exclusion chromatography(SEC). Thankfully, I could get a clear peak for my target protein and separate it from...
24 April 2019 4,503 12 View
I have one day old chick with mycoplasma gallisepticum and Synovi ELISA titters are 1,743 and cv 68 / 3,608 and cv 30 respectively Are these titters indicates infection or maternals antibodies...
24 April 2019 3,173 3 View
Hi, I am developing a sandwich ELISA for Rubella viral quantitation. I'm using Rubella Glycoprotein E1 for coating and a polyclonal HRP conjugated Ab for detection. In my last experiments, I...
22 April 2019 8,317 3 View
I calculated LOQ and LOD for ELISA but it seems that the LOQ smaller than LOD. According to EPA (1996) and Armbruster & Pry (2008), LOQ cannot be lower than LOD. Where did I could possibly go...
22 April 2019 913 3 View
Dear colleagues, I would like to ask you if you have any experience how to use data from ELISA analysis when my standard curve is not as showed in the manual? I have many standard curves that have...
18 April 2019 422 4 View
Good morning all, My question is just like in title. I was using EDTA when collecting mouse blood, and most of ELISA kits are dedicated for plasma gained from blood collected with citrate or...
16 April 2019 2,870 2 View
Hi! I am searching for ELISA Polycarbonate ( the wells should entirely be made of polycarbonaTe) microtitleplates. does anyone know a seller ? thanks
09 April 2019 1,415 2 View
I'm to test LTB4 on human serum samples using Cayman Chemical kit cat#520111. Has anyone done this and if so did you need to purify the samples ?
04 April 2019 456 1 View
I am growing adherent primary epithelial cells in supplemented DMEM/F12 and testing the supernatant for inflammatory cytokines. I purchased a commercial sandwich ELISA to test for analyte....
04 April 2019 7,777 3 View
I want to estimate alpha amylase in the blood and saliva samples. Can anybody share the simple protocol which can be performed using spectrophotometric method (not ELISA) through which we can...
04 April 2019 6,355 1 View
Dear all, In the lab I currently work at we have rabbit sera, immunized against antigens of interest. I have isolated IgGs from these with a column (Protein A IgG purification kit from Thermo...
04 April 2019 4,185 5 View
currently we are working on various analgesics and anti-inflmmatory drugs for mechanistic evaluation. In this regard we are looking for procedure to evaluate PGE2 from paw (carragenan method),...
04 April 2019 9,433 3 View
Hello, For the measurement of the prostaglandin E2 in J774 macrophage cells supernatants, i found 2 Kits: Express ELISA kit, and Monoclonal ELISA kit. I would like to know the difference between...
04 April 2019 4,224 1 View
May I know what else can I do to prevent this from happening? I tried 3 times with the same Elisa and I always get a high background for all my standard, control and samples despite followed the...
04 April 2019 600 5 View
Recently, I use CpG oligonucleotide for IL-12 ELISA. I made CpG RCA(Rolling Cycle Amplification) product and treat 5ug/ml to Bone Marrow Dendritic Cell. After 48h later, I did ELISA by using...
04 April 2019 1,423 5 View
We plan to measure NFkB activation in fibroblasts in vitro without using western blots. If anyone has an experience with the 2 following methods, which is preferable: measuring phospho- vs total-...
04 April 2019 4,406 2 View