I had control cells and performer ELISA analyses group (time 0). I stimulated cells with substance A and B and cultivated for 1, 2 and 3 hours. On each cells I performed ELISA. I have results from groups:
time 0, no substance A or B
time 1, substance A
time 2, substance A
time 3 substance A
time 1, substance B
time 2, substance B
time 3 substance B
The problem is I do not have groups:
time 1, no substance A or B
time 2, no substance A or B
time 3, no substance A or B
Can I do two way ANOVA (factors: time and substance), despite some groups are missing, to check how the factors impact ELISA results and do post hoc test?