A forum to address questions regarding enzyme-linked immunosorbent assay, most commonly known as ELISA. | Contact experts in ELISA to get answers
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Questions related to ELISA
I won´t do an ELISA assay but I'll do a biochemical assays, like lipase activity assay, lipid peroxidation or total carbohydrate assay. Can I use the ELISA reader without filters? What is the...
07 July 2019 6,304 6 View
Thehuman kidney tissue samples will be used later for immunohistochemistry, ELISA, gene analysis, etc. Should I freeze it in solution or not?
21 June 2019 3,190 3 View
I use 1% BSA for blocking ELISA plates. However, even after blocking for 3 hours, I still get background noise for some of the cytokines but not all. What can be the reason for this background...
20 June 2019 10,072 3 View
I am trying to develop and optimize an ELISA. To do so I will be performing a checker board titration to optimize what dilutions would work best. For the capture antibody I was thinking about...
17 June 2019 5,647 4 View
I have 0.05 ml of an HRP conjugated antibody that is at a concentration of 1.0 mg/ml. The company reccomends starting at a 1:5,000 dilution, but proper working dilution needs to be optomized....
06 June 2019 2,933 2 View
I am treated PBMCs with various compound to observe cytokine expression using ELISA, however, I am getting high expression in my untreated control cells as compared to my media-only control. I am...
06 June 2019 4,473 14 View
Need recommendations please.
06 June 2019 3,211 6 View
I have done a qualitative ELISA-analysis, and I need some help with my calculations. I tested my protein against different receptors. I used a plate reader for the absorbance (450nm) of the wells,...
06 June 2019 2,795 2 View
06 June 2019 9,562 4 View
06 June 2019 3,470 3 View
Hello everyone, I have a question concerning IL-10 secretion by murine M2 macrophages. I am currently doing an ELISA test to evaluate the secretion, but first I would like to know what is the...
06 June 2019 9,444 3 View
I've been using the Elisa oxytocin kit by Enzo to measure plasma oxytocin, but the results suggest that my samples' concentrations are too low. Cusabio has a mouse-specific oxytocin elisa kit, but...
06 June 2019 1,517 2 View
Hi, I'm doing a preliminary study with ELISA where I would spike in 2 drugs purchased from Selleck at concentrations that are well within sensitivity of the kit. We optimized the kits to...
06 June 2019 986 10 View
I'm trying to standardize a buffer in which my biotin conjugated and HRP conjugated molecules (antibodies and antigen) stay stable for longer time. My assay for detection of HIV works well with no...
06 June 2019 7,257 3 View
06 June 2019 2,327 13 View
02 June 2019 5,180 2 View
Hi all, I recently did a project where I did carboxyl-to-amine immobilisation with a monoclonal interferon gamma antibody (human ELISA development kit, Mabtech). I had carboxyl groups on a carbon...
31 May 2019 6,466 7 View
Hi everyone, I am trying to detect Amyloid oligomers in brain tissue extract of APP mice by ELISA kit but the outcome is mostly undetectable. I am using IBL-Elisa kit: Amyloid Beta...
29 May 2019 5,231 2 View
I performed assays in microplates for the first time to determine enzymatic activity, but I made a mistake with the reading. The recommended wavelenght for the assay is 550 nm. I used an ELISA...
07 May 2019 3,253 11 View
Hello, We measured the level of pge2 in the culture supernatant of rat cells using a competitive elisa kit. the problem is that we got values out of range, which means that the rate of pge2 was...
06 May 2019 3,541 2 View
05 May 2019 5,610 2 View
05 May 2019 8,223 8 View
Several approaches are used for the determination of detection limit in analytical chemistry. I wonder which is the best approach for ELISA data.
05 May 2019 9,173 3 View
I have been doing ELISA to detect Ab in our serum samples and every time i got color in my negative control (uncoated wells and wells that only have secondary Ab) as well as the absorbance for...
05 May 2019 7,246 4 View