A technique using antibodies for identifying or quantifying a substance. Usually the substance being studied serves as antigen both in antibody... | Contact experts in Immunoassay to get answers
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Questions related to Immunoassay
So I'm new to lab research and learning Western Blots these days. I ordered my secondary antibody and it arrived in a powdered form. Here is the...
28 January 2021 5,579 3 View
Hi to everyone! Does anybody know optimal buffer for detecting HIV-IgG with lateral flow immunoassay using saliva as a specimen type? I think that i need a buffer for LFIA becouse saliva too...
17 November 2020 1,663 3 View
I am working with multiplex immunoassay. As I am new into multiplexing I am facing some problem. I have coupled Protein X to carboxylated magnetic beads (BioRad) using EDC-NHS. Now, while...
09 November 2020 10,033 7 View
Hi everyone, I plan to make my own cortisol standards for immunoassay. Hydrocortisone (cortisol) is soluble in organic solvents, such as ethanol, methanol, DMF etc., but I'm not sure which one...
02 September 2020 3,565 4 View
I did competitive lateral flow immunoassay using gold bridge method. Antigen-fluorescence bead and immobilized antigen on the PMMA membrane is no problem to measure the optical absorption at 37...
25 August 2020 2,822 4 View
I'm working on a lateral flow immunoassay. Conjugated to my colloidal gold is goat anti-bovine IgG. For the control line, I immobilized bovine IgG. My boss suggested to use anti-goat IgG to...
11 August 2020 5,158 4 View
When I defrost my plasma samples from -80, I do so on ice, slowly. Then I centrifuge the samples. Upon centrifugation, the plasma samples will have a white sticky substance layer at the top, but...
31 July 2020 5,040 2 View
Despite using published protocols for the use of AMD3100 in mice, is there any way to prove via a quick assay, either immunohistochemical in any mouse tissue or an immunoassay in the circulation,...
24 June 2020 1,930 2 View
We use a primary amine surface to bind our antibodies. Thereafter we use BSA and other protein blockers for blocking. The results are not really great, and vary quite a bit on repeats. We wanted...
27 April 2020 3,259 1 View
So far, RT-PCR testing has been the frontline response to the COVID-19. PCR testing is considered as the gold standard for detecting COVID-19 and was designed by WHO, soon after the virus was...
22 April 2020 1,270 16 View
We need to lyse the viruses for downstream immunoassays, and so it is important that the proteins are not damaged. I have only lysed cells and tissues before for SDS-PAGE, would a similar protocol...
13 April 2020 2,260 3 View
Usually, RT-PCR or qPCR based test assay is performed for the diagnostic tests, which seems a little bit time-consuming. On the other hand, some rapid tests based on IgG-IgM interaction have been...
22 March 2020 4,411 5 View
I am using a magnetic bead protocol which involves a step of non-denaturing elution to remove bead-antibody from antigen. The protocol calls for 20ul 50 mM glycine pH 2.8, and I have been using...
19 March 2020 1,823 4 View
A competitive immunoassay technique is used. This involves the reaction of anti-HBc in the sample with hepatitis B core antigen (HBcAg) coated wells. Unbound material is removed by washing....
17 March 2020 10,014 3 View
We have used a competitive immunoassay to determine the concentration of a neuropeptide in CSF samples. According to the literature, the levels of the neuropeptide should fit properly to the...
16 March 2020 6,665 3 View
I'm trying to change the limit of detection (L.O.D) of my target analyte for an LFIA (competitive) test strip. So far, I have attempted to change both the volume of my conjugate antibodies on the...
01 January 2020 5,263 3 View
WHO has established a reference preparation for hCG (currently the fourth International Standard, with an assigned content of 650 IU/ampoule) and a reference preparation for hCG-beta (currently...
31 December 2019 5,003 3 View
Electrochemical sensors have an inherent advantage for being simple, cheap (glucometers), especially for nucleic acid assays. Yet there are very few health devices in the market for point of care...
23 December 2019 2,713 3 View
I'm trying to develop a chemi-luminescence based immunoassay for HIV antibody detection. For the purpose I'm using negative as well as positive control as a quality control. Currently, I'm using...
12 December 2019 3,177 2 View
Can we use Fluorescein isothiocyanate (FITC) in primary antibody conjugation for fluorescent immunoassay, why?
12 December 2019 1,891 4 View
I have modified an affinity chromatography column coated with Protein G to efficiently separate IgGs. Is it possible to elute the bound IgGs by adjusting temperature instead of utilizing elution...
21 October 2019 8,782 2 View
Lateral Flow Immunoassay system were used for newborn screening test and these systems consist of some parts including plasma separation membrane part, I found lots of plasma separation membrane...
10 October 2019 6,505 1 View
I am trying to develop a new methodology for ELISA immunoassay. Systems using horseradish peroxidase (HRP) are already well developed and widely used either with chromogenic, chemifluorescent or...
09 September 2019 8,409 3 View
I have gold coated quartz crystals that I want to bind with antibodies to perform an immunoassay. The crystals are quite expensive and I would like to be able to clean the surfaces and reuse them.
27 August 2019 1,659 1 View