A forum to address questions regarding enzyme-linked immunosorbent assay, most commonly known as ELISA. | Contact experts in ELISA to get answers
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Questions related to ELISA
Hello. Our incubator for primary cultures is broken but I need to isolate some islets for both qPCR and estimating the insulin content. Has anyone performed either of these techniques on freshly...
11 November 2019 8,608 3 View
I need to dose the ethylene hormone with an ELISA kit but I can't find any company that markets this kit. Can you help me?
11 November 2019 8,496 2 View
Good afternoon, Currently my team is performing animal studies with majority of the time using rats of different strains. Our latest experiment was a weight loss study with diet-induced obesity...
11 November 2019 2,118 2 View
When doing T-cells activation assay, for example looking for CD25 ,CD69 expression or ELISA for cytokine release, is there a difference between the coated antibody or coated protein stimulation...
09 November 2019 795 3 View
In sandwich ELISA, a capture antibody attach to the 96-well plate prior to the antigen. Can the similar form be used in western blotting? In this way, a capture antibody attach to the...
03 November 2019 4,122 3 View
Hi, I am currently producing a recombinant protein from yeast Pichia pastoris. The medium used in the bioreactor is the Basal salt medium supplemented with PTM1 salts. I have noticed that when...
20 October 2019 8,552 5 View
As far as I know, IDO1 is expressed inside the cells (ie not a secreted protein). I am afraid that serum/plasma is not a suitable sample type or the level of IDO1 may be too low to be detected in...
15 October 2019 739 3 View
10 October 2019 3,950 3 View
10 October 2019 3,237 5 View
I am currently carrying out my ELISA assays and I want to calculate the CV% for each of my samples. Should the CV% be calculated based on the replicate OD values or based on replicate...
10 October 2019 390 4 View
In order to determine the function of macrophage subtypes, we measure the amount of cytokines they produce by ELISA. However, we add recombinant IL-10 to induce the subtype we want. This makes...
10 October 2019 8,526 4 View
Hi all, I'm hoping you might be able to help me with a problem I've been having. I'm attempting to perform an ELISA to determine which domain of fibronectin one of my proteins binds to. To...
10 October 2019 6,299 8 View
Hi folks, I am looking for an ELISA, which detects rat IFNa subtypes, especially subtype 2. So far, i couldn't find one. Most measure IFNa1 or do not even state which subtype. I'd appreciate any...
10 October 2019 4,530 1 View
I am trying to measure TGF-B levels in lung homogenates using ELISA. How do I normalize the results to lung homogenate total protein concentration determined with the BCA protein assay?
10 October 2019 4,652 3 View
I usually perform short-term treatments (6h) and so I use serum free medium, but in a new protocol I have to treat for 5 days Caco-2 cells grown on Transwell membranes. In this case should I use...
10 October 2019 7,631 4 View
We are encountered with a phenomenon when T3 FREE was estimated in fish plasma by ELISA (DRG tests): some samples are shown no significant differences in total and diluted plasma (in PBS pH7.4,...
30 September 2019 8,948 3 View
I'm going to perform an ELISA for fish GnRH (using CUSABIO). The "Technical Tips" pamphlet that came with the instructions manual says I need to centrifuge all reagents before use "as to...
18 September 2019 5,706 2 View
We are interested in comparing several different analytes in plasma. Certainly, ELISA is more expensive than multiplex technology. Multiplexing saves plasma, time and money. However, we would like...
10 September 2019 7,746 3 View
Can commercial Competitive ELISA Oxytocin kits measure/pick up synthetic oxytocin (used in clinical practice)?
10 September 2019 6,617 3 View
I have coated a ELISA plate for sandwich ELISA, at 4C overnight. But I was missing a reagent next day, how long can the plate stay at 4C until it is reused later?
10 September 2019 6,923 3 View
09 September 2019 3,956 3 View
the drug I use is zinc oxide nanoparticles. when they are dispersed the, the obtained solution is not clear and this affects how their MIC is read on the ELISA reader. i tried to use DMSO and the...
09 September 2019 4,655 3 View
Is it possible or not?
09 September 2019 3,608 1 View
09 September 2019 511 2 View