A forum to address questions regarding enzyme-linked immunosorbent assay, most commonly known as ELISA. | Contact experts in ELISA to get answers
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Questions related to ELISA
I detect the plasma AngII concentration by AngII ELISA kit (ENZO), the results show that plasma AngII decrease in AngII infusion mice. I check with the kit and find that the kit could recognize...
01 January 2021 9,063 3 View
We are performing an ELISA experiment to determine the binding of Complement antibodies against P. aeruginosa. We have pNPP substrate (Thermo Scientific cat # 34045) instead of pNPP tablets. We...
23 December 2020 2,194 3 View
I have measured levels of GLUT1, GLUT3, and GLUT4 in the mouse hippocampus and cortex using ELISA kits. I'm trying to find typical concentrations (in ng/mg total protein) of these proteins in the...
22 December 2020 4,789 2 View
Anyone know of a way to efficiently lyse small amounts of DRG tissue for a non-sensitive competitive ELISA analysis? Looking to steer away from chemical lysis as the protein in question will be in...
15 December 2020 9,463 3 View
We want to test levels of Reg3gamma in human fecal samples. Is there an ELISA kit that people prefer? Thanks for your help.
10 December 2020 4,574 2 View
Hi everyone, I'm gonna try to detect the IL-10, IFN-y, TGF-beta1 level in the human serum by ELISA. I bought the ELISA set from the BD contain Capture Ab, Detection Ab, Enzyme reagent, Standards....
10 December 2020 3,541 5 View
I’ve been doing Sandwich Elisa with Greiner Bio-One High Binding Standard ELISA plates (clear polystyren, microlon600 surface treatment, Fisher scientific). It didn’t work well, but we’ve figured...
10 December 2020 408 2 View
Hi all, I have frozen (-20C) samples of whole blood from mice from a recent experiment. I need to find a way to thaw them so as to extract sera for an ELISA, while minimizing hemolysis and...
09 December 2020 1,119 3 View
Hi All, I am planning to measure HIF expression with different cellular conditions using ELISA kit, from your experience what is the ideal number of cells used for this type of experiment?
08 December 2020 3,181 3 View
Dear colleagues! I plan to check cytokine production by my WT and KO Tregs following activation, particularly, IL10 and TGFb (TGFb1 or LAP, exactly) as these are reported to be the major...
03 December 2020 9,564 5 View
Dear scholars, Our research is currently involved with hybridoma technology. We have isolated some original clones after fusion and we are preparing the subclone step to find some true positive...
02 December 2020 1,375 3 View
Background: I performed a DAS ELISA reaction at 405nm of 129 bacterial isolates (in addition to using controls) to identify the presence of my target bacterial strain. For my positive control, I...
25 November 2020 5,925 4 View
I would like to ask if anyone knows if concentrations of Urea 3M and 8M can interfere with protein purification at Ni-NTA and Glutathione Sepharose 43 resins. Another question, Urea, at 3M and 8M...
18 November 2020 5,202 3 View
Is hyaluronic acid content analysis by carbazole assay applicable to cell experiments? I'm looking for hyaluronic content analysis not use ELISA kit.
18 November 2020 7,188 2 View
I intend to open this thread with an unconventional inquiry. As part of a hypothesis to check for metabolic changes in patients treated with antipsychotics, we drew blood in EDTA tubes 5-19...
17 November 2020 8,144 3 View
Hello, I have done sandwich ELISA and two curves are attached. curve (A) was greatly generated and gave accurate QC values. But, the graph B was not appropriately obtained. The two experiments...
14 November 2020 8,536 3 View
I’m wondering if you can help with this issue. Currently working on In-house ELISA using Nunc thermo scientific plates and Gene script spike protein it started to give too much signal. I thought...
10 November 2020 5,644 5 View
I wand to estimate antibody in serum by ELISA. In order to obtain clear serum, is it ok to spin serum at 7000 rpm (without refrigeration) for 5 minutes? Thank you in advance.
09 November 2020 2,621 5 View
I am looking for a skilled personnel in Kuwait University that is familiar with antioxidant studies using ELISA kit or other techniques on rat's brain homogenate, particularly the hippocampal and...
05 November 2020 5,587 1 View
So, I am unable to find a direct answer but essentially I want to use an ELISA protocol to measure the amount of ubiquitylated histone H2A in my mutant cells. I was wondering if I could get a kit...
03 November 2020 6,823 2 View
Dear all, I am trying to measure IL-1beta levels in the ascitic fluid of MSU induced peritonitis mouse model by using Duoset IL1B ELISA(R&D). However, under all conditions, the absorbance...
29 October 2020 7,371 3 View
Hello! Our lab has developed a standard curve with known concentration that we use to determine our ELISA concentrations. We then multiply by the dilution factor for our final concentration....
26 October 2020 8,822 6 View
i searched more about using ELISA to diagnosis autoimmune disease and what i found is that depends on type of the disease. the target will be an antigen specific for the autoantibodies presence in...
26 October 2020 5,678 3 View
Many assays, including Cell-based assays, QPCR, some ELISA test, rely on the presence/absence of fluorescence. Diagnosis applications about cancer or antiobiotic resistance uses fluorescence ratio...
22 October 2020 1,239 3 View