What is the best way for quantification of a FlAG-tagged protein?

Shyambabu Chaurasiya @Shyambabu-Chaurasiya

12 January 2021 1 9K Report

Anti-FLAG ELISA can be used but the problem is that we do not have an antibody against our protein. Any suggestion will be appreciated.

Thank you

Sima Lev

You can either use anti-flag antibodies in WB, there are several good commercial (Sigma for example) and also an anti-flag affinity resin for affinity purfication from cell lysate. It is very good - Flag-affinity gel (https://www.sigmaaldrich.com/life-science/molecular-biology/molecular-biology-products.html?TablePage=14842111)

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