A forum to address questions regarding enzyme-linked immunosorbent assay, most commonly known as ELISA. | Contact experts in ELISA to get answers
1,301 views 2,618 posts
Questions related to ELISA
The method/apparatus does not have to be very accurate. The more simple, the better. I have looked into ELISA and it is not feasible with the environment that I am working in (high school lab)....
09 September 2019 7,180 6 View
I have researched and understand the basic principle and approach of a checkerboard titration. In a recent discussion with my manager, several questions have come up: These relate specifically to...
09 September 2019 4,337 2 View
Can commercial Competitive ELISA Oxytocin kits measure/pick up synthetic oxytocin (used in clinical practice)?
09 September 2019 1,898 4 View
We are encountered with a phenomenon when T3 FREE was estimated in fish plasma by ELISA (DRG tests): some samples are shown no significant differences in total and diluted plasma (in PBS pH7.4,...
09 September 2019 4,175 3 View
Hi, I was thinking if it is possible to do a immunoassay using a partial peptide (the same I used to produce the antibody for that same immunoassay) as a standard? Also would it be useful in a...
09 September 2019 419 4 View
We are interested in comparing several different analytes in plasma. Certainly, ELISA is more expensive than multiplex technology. Multiplexing saves plasma, time and money. However, we would like...
09 September 2019 1,098 3 View
I want to track relative increases/decreases in cytokine during iDC and mDC cultures. I was thinking I could use a cytokine array kit as they are easy to use and off the shelf/don't take any...
09 September 2019 9,792 5 View
04 September 2019 7,443 4 View
Hi all I was trying to perform a ELISA, in which I coat the plate with strep, then add in biotin conjugated peptide, then human serum and use anti-human IgG to detect antibody level to the...
21 August 2019 4,709 3 View
Recently we evaluate the serum IL-6 level in patients after radiotherapy . Unfortunately 4 of 7 samples are negative. we used the thermo fisher ELISA kit for this aim and 100 microliter of...
21 August 2019 8,680 4 View
I am developing and optimizing an ELISA and I was jsut wondering how you determine what the best concentrations of antibody and antigen are based off the OD values. I have performed checkerboard...
19 August 2019 7,740 3 View
I am trying to develop and optimize my own indirect sandwhich ELISA by performing cherkerboard titration. I am currently checking different dilutions of the capture antibody against the antigen...
09 August 2019 5,050 7 View
Using a fluorophore rather than a chromophore for ELISA detection seems like an obvious solution to increasing ELISA sensitivity, but I'm struggling to find work done in this area. Can anyone...
08 August 2019 8,716 6 View
Hi there! We are applying L.lactis for expression of a heterogeneous antigen using a cell wall anchor subunit. Cloning process has been verified and expression optimization is up now but I'm...
08 August 2019 6,115 2 View
Neutrophils will be cultured and treated for a fixed period of 9-12 hours and cytokine and chemokine secretion determined by ELISA. What method is used to determine cell viability at assay...
08 August 2019 2,569 3 View
I have tried different concentrations of LPS (1, 10 ug/mL), on THP-1 cells differentiated by PMA (1 x 10^6 cells/mL, or 2 x 10^6 cells/mL), at different time points (3h, 6h and 24h). There is no...
08 August 2019 4,881 4 View
Hi. I'm planning to perform ELISA to find amino acid residues which directly bind to the protein X (protein of interest). You may see the design of the experiment in the picture that I posted....
08 August 2019 2,112 3 View
I am trying to avoid any chemicals in my lysate of transfected HEK cells because I intend to use the supernatant of the lysate for ELISA. Therefore I wonder if I can use water or PBS alone for...
08 August 2019 7,071 7 View
08 August 2019 8,237 8 View
Hi, I have snap-frozen (isopentan-bath) mouse kidneys stored at -80°C and embedded in OCT. How can I de-embedd them to use for a Nephrin ELISA? Thanks.
08 August 2019 5,338 1 View
Hey! I am developing an Elisa against anti-GRP78-antibodies for measuring titers in serum samples. I want to coat the plate with GRP78 in a carbonate buffer and detect the auto antibodies with...
08 August 2019 557 6 View
08 August 2019 6,404 4 View
I have a standard curve for an ELISA, and want to find unknown concentrations from absorbances. my polynomial equation is: y= - 1e-06x^2 + 0.0032x + 0.2562 How can I solve this to find analyte...
08 August 2019 3,581 3 View
We have an in house ELISA, how do you calculate unknown concentrations from optical density values? We produced a standard curve but don't know whether to use sigma plot or excel. Also, Which...
08 August 2019 10,032 6 View