A forum to address questions regarding enzyme-linked immunosorbent assay, most commonly known as ELISA. | Contact experts in ELISA to get answers
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Questions related to ELISA
Hi all I was trying to perform a ELISA, in which I coat the plate with strep, then add in biotin conjugated peptide, then human serum and use anti-human IgG to detect antibody level to the...
08 August 2019 4,650 3 View
Recently we evaluate the serum IL-6 level in patients after radiotherapy . Unfortunately 4 of 7 samples are negative. we used the thermo fisher ELISA kit for this aim and 100 microliter of...
08 August 2019 2,749 5 View
Hi. I'm planning to perform ELISA to find amino acid residues which directly bind to the protein X (protein of interest). You may see the design of the experiment in the picture that I posted....
08 August 2019 2,683 3 View
I am trying to avoid any chemicals in my lysate of transfected HEK cells because I intend to use the supernatant of the lysate for ELISA. Therefore I wonder if I can use water or PBS alone for...
08 August 2019 2,280 5 View
Hi there! We are applying L.lactis for expression of a heterogeneous antigen using a cell wall anchor subunit. Cloning process has been verified and expression optimization is up now but I'm...
04 August 2019 7,532 2 View
I am developing my own ELISA for Factor V in mice, however I am having a hard time figuring out how I will be able to run a standard curve. I know people usually use a lypholized powder at a known...
29 July 2019 8,711 3 View
I tried using an ELISA kit to measure ACE2 in human plasma, however there was no detection. I would like to know if there are more sensitive and easily accessible methods for measuring ACE2?
28 July 2019 8,240 1 View
Hello, I did multi-analyte ELISA to detect the concentrations of several cytokines, but how should I calculate the concentration as each well represents a different sample and a different...
12 July 2019 9,799 2 View
Hi All, I'm trying to express a rAb in CHO or HEK293 cells and I am having an issue getting functional antibody. WB shows bands for HC and LC under reducing conditions and non-reduced shows a...
11 July 2019 8,749 4 View
I have found in the literature that H154 is a specific inhibitor of TLR9. For this reason, I performed an experiment using 10uM H154 in macrophages (PMA-differentiated THP1 cells) before...
11 July 2019 4,643 2 View
I am looking into getting a plate washer for performing ELISAs to help with consistency and I was wondering if anyone had any reccomendations.
10 July 2019 290 3 View
Hi all I have a question. I want to validate an ELISA assay. To do so I want to have some samples spiked with 25 ng/ml protein (final concentration) and some not spiked. Here is my question: I...
08 July 2019 5,243 2 View
07 July 2019 6,788 4 View
07 July 2019 9,245 2 View
I've tried using PBS and carbonate buffer to coat. Tried 2 hours at RT as well as overnight at 4C. GFP washes away after washing with PBS/.05%Tween 20. Any ideas how to coat GFP to the plate?
07 July 2019 4,022 3 View
I am doing my master’s thesis about cytokines and I’m using the R&D systems DuoSet ELISA kit. My problem is that I don’t know what kind of standard curve is should be using. The plate reader...
07 July 2019 9,929 4 View
07 July 2019 5,060 2 View
Dear researchers/professors, For my master's thesis I prepared insulin loaded nanoparticles (100 IU/kg) and gave them to rats via oral route. To compare my formulation I also gave insulin sc (2...
07 July 2019 8,274 7 View
We are currently running multiple analyses on our culture media (to test what the cells were expressing , DNA quantification, etc..). We have aliquotted several for each type of sample and stored...
07 July 2019 3,295 4 View
07 July 2019 2,749 2 View
07 July 2019 779 4 View
07 July 2019 5,056 2 View
We have been testing few ELISA kits with human plasma samples, and tried to improve their sensitivity (or signal recovery) by adding to the plasma few concentrations of TRITON X-100 (from 0.4 up...
07 July 2019 1,395 2 View
07 July 2019 345 3 View