A forum to address questions regarding enzyme-linked immunosorbent assay, most commonly known as ELISA. | Contact experts in ELISA to get answers
1,300 views 2,618 posts
Questions related to ELISA
Hi researchers, I am very pleased to ask my question. How the specificity o and sensitivity of the sandwich ELISA method is determined ? Many thanks
29 March 2020 7,795 4 View
I am looking for sandwich Elisa kits for human CTNND1, and I have found the kits from the above companies. However, our group are not familiar with these companies, and I cannot find publications...
03 February 2020 6,254 1 View
Hello, I am using an in-house competitive ELISA for the detection of a small peptide in the various samples (plasma, cell culture supernatants etc.). For the method's needs, polyclonal rabbit Abs...
02 February 2020 922 3 View
I have cultured cells which were exposed to a certain drug in a 24 well plate at different conc and time points. At the end of the experiment, I used Trizol for RNA isolation. I removed media from...
02 February 2020 9,659 3 View
For how long and at what temperature urine samples can be stored to measure COTININE Levels using ELISA technique?
02 February 2020 3,042 1 View
Hi everyone - I'm setting up an ELISA for the first time and mostly figuring it out on my own. I'll be analyzing secreted proteins in cell culture supernatants. Looking through protocols and...
02 February 2020 9,188 3 View
a marker taking into consideration the specificity, sensitivity and cost of measuring it.
01 January 2020 4,376 3 View
Why do we get Negative OD value in ELISA ? ELISA for the detection of IgG for HCV.
01 January 2020 1,990 3 View
Dear people, I am analysing some data from an ELISA to determine further steps in my research. Unfortunately, some values fall (quite far) outside the standard curve (non linear). Nevertheless,...
01 January 2020 738 3 View
Hi everyone, last time I was doing an ELISA without exposing it to the light and before I added the substrate it was turning blue. Do you have any idea or suggestion of a better substrate?, the...
01 January 2020 7,402 6 View
All ELISA kits in most researches that I have read are used for serum samples. I wonder if there are any available kits for tissue samples as beef meat for further confirmation of presence of...
01 January 2020 8,127 2 View
ELISA is an indirect method of diagnosis is it easy to to be used and replace direct microscopic examination Which may be need well trained experienced staff !!
12 December 2019 2,182 3 View
I want to check tumor marker concentration in rat serum. I use 30 rat dividing to 6 group. How many wells do i need in ELISA?
12 December 2019 6,608 2 View
Hello, I am using four parameter logistic (4-PL) curve fit (using GraphPad Prism8) to interpret my samples concentration after each ELISA assay. However, some of my samples with ODs less than the...
12 December 2019 2,295 3 View
I have been trying to get my ELISA running for a while now and at the moment don't know what else to try. I am trying to detect Flotilin-1 in lysates of human monocytes. Primary antibody is...
12 December 2019 1,269 3 View
Yellow colour, before the reaction is stopped, is due to the formation of oxidized TMB, which isTMB(2+). The reaction is stopped by degrading HRP, but are there any further oxidations happening?...
12 December 2019 526 3 View
Hello everyone. please assist me in the following issue. I am currently developing a custom sandwich ELISA for the detection of antigen x. I am using two monoclonal antibodies with a secondary...
21 November 2019 9,767 1 View
Hello. Our incubator for primary cultures is broken but I need to isolate some islets for both qPCR and estimating the insulin content. Has anyone performed either of these techniques on freshly...
19 November 2019 1,476 3 View
Good afternoon, Currently my team is performing animal studies with majority of the time using rats of different strains. Our latest experiment was a weight loss study with diet-induced obesity...
15 November 2019 7,233 2 View
I would like to perform an ELISA from the cell culture supernatant of immortalized human hepatocytes (IHH) cells. When I attempted the ELISA for a first go, the apoB concentration was on the lower...
14 November 2019 1,406 3 View
Hello, I am setting up the culture conditions to quantifiy collagen I production in a primary culture of dermal fibroblasts. I am using and ELISA kit to quantify and I get signal in my basal...
13 November 2019 5,829 2 View
I am using IgM Mac ELISA for diagnosis of viral infection in our laboratory. I am strictly following the protocol as per the instruction from the manufacturer, but unfairly getting the high...
11 November 2019 6,450 1 View
Is there any other way than ELISA to measure PG E2 ?
11 November 2019 10,067 3 View
Hi, I did a test with a sandwich ELISA where I incubated an antigen (human serum) in a coated microtiterplate. After the incubation I transferred the human serum to a new wells and incubated that...
11 November 2019 423 3 View