A forum to address questions regarding enzyme-linked immunosorbent assay, most commonly known as ELISA. | Contact experts in ELISA to get answers
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Questions related to ELISA
Dear Colleagues I am interested in evaluating the concentration of SERUM succinate dehydrogenase in a certain type of cancer. I found an ELISA kit for it however, I can barely find any papers...
17 October 2020 693 1 View
Hello everybody, I immunized mice successfully with NP-KLH ( 4-Hydroxy-3-nitrophenylacetyl hapten is conjugated to KLH (Keyhole Limpet Hemocyanin) lysine through amide bonds). I let them sit for...
16 October 2020 6,110 3 View
We are doing DAC ELISA against plant viruses. I have following queries. Controls that we are using are as follows. Buffer control, Healthy leaf samples, disease infected sample (+ve sample), and...
15 October 2020 1,720 2 View
I am working on recombinant protein expression in E. coli. Recently, I thought I had expressed a certain protein successfully as I could see a band of the expected size - 17 KDa (this band was...
15 October 2020 5,271 4 View
We have been looking for the fueling effects of cytomegalovirus on HIVpositive patients. patients serum was tested for CMV by PCR and ELISA for IgM. We found discrepant results, that IgM positives...
13 October 2020 5,212 2 View
I am using a new antibody for protein pfs48/45 in a sandwich ELISA using alkaline phosphatase. The standard curve looks really good when diluting the samples in ESF-AF media however when I have...
09 October 2020 6,430 2 View
Hello, I am running a sandwich-type ELISA for which the readout is absorbance at 450nm. The ELISA measures protein binding which I am screening for compounds to inhibit. The 570nm...
06 October 2020 9,950 6 View
I am confused as to which ELISA kit type to use for a colon cancer work, Sandwich or Competitive and why? Thank you
06 October 2020 6,770 2 View
Want to perform ELISA on whole blood instead of separating serum
04 October 2020 3,029 4 View
Dear research community, I'm looking for a reliable human ApoB100 ELISA to measure ApoB in VLDL particles which were isolated by density gradient ultracentrifugation. Are there any...
29 September 2020 2,899 2 View
How to determine transglutaminase content in fish and fishery products? Any spectrophotometric methods other than ELISA and PCR?
28 September 2020 8,295 4 View
I want to analyze certain cytokines in plasma samples and coat ELISA plates with monoclonal antibodies specific to the cytokines, how do I go about preparing my ELISA microplate ie. from coating...
28 September 2020 3,938 3 View
I am looking to quantify both human serum and skeletal muscle homogenate betaine concentrations via ELISA or colorimetric assay kits. Has anyone had a good experience with any products...
25 September 2020 4,744 3 View
I need to know how to convert micrograms of antibodies into ELISA Units.
22 September 2020 3,417 3 View
I've been trying to detect mScarlet protein purified from E. coli via an ELISA assay. My protein has two tags: an N-terminal Strep-tag and a C-terminal His-tag. To do the ELISA I've used...
21 September 2020 888 2 View
I'm trying to standarize in house ELISA for viral detection using recombinant protein. How much antigen should be add per well in 96 well plate? There is a range of µg that is commum used?
20 September 2020 622 3 View
Hello wise scientific community, I am trying to measure the concentration of Spike Protein ( whole protein) expressed by my cell line transfected with a pC DNA 3.1. I can see a nice band by...
18 September 2020 7,403 4 View
I am using an organotypic hippocampal slice culture where we are exposing our slices to alcohol with subsequent alcohol withdrawal. I want to make sure I am capturing the right time to assess...
17 September 2020 7,144 2 View
I'm trying to measure IgG level from plasma samples that I collected weekly from my animal experiment to see how IgG level changes throughout the study. Since the level will be different in...
16 September 2020 2,011 3 View
Hello, I recently performed Kit based ELISA for 2,3 DPG on plasma samples of control and hypoxia treated rats. [CAT. NO. E1562Ra, Bioassay technology laboratory] However, the results I obtained...
08 September 2020 3,434 2 View
Hello, For a project, I need to use frozen PBMC with rare T cells (hopefully) and expand those T cells with synthetic peptides. After the expansion, I will confirm the activation of T cells with...
31 August 2020 7,420 3 View
we are developing a standard competitive assay for small bio-molecule. we are regularly noticing a hook kind of higher signal for one of the middle calibrator (Calibrator concentration and purity...
12 August 2020 842 1 View
Hi, I am trying to do a direct ELISA for mouse IGF-1 (only standards for now), however I am facing technical issues with obtaining a lower blank value and a linear graph. I am doing the following...
04 August 2020 6,363 11 View
Dear all, Please I need to detect some chemical molecules in water using ELISA and to develop it I need an important quantity of reagents. I want to reduce the number of experiment, I asked aroud...
01 August 2020 3,229 1 View