Immunology

Immunology is a broad branch of biomedical science that covers the study of all aspects of the immune system in all organisms. It deals with the... | Contact experts in Immunology to get answers

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Questions related to Immunology

Can probiotics (eg yoghurt) activate the anti-aging gene Sirtuin 1 with relevance to the treatment of Alzheimer’s disease?

The relevance of the Microbiota-Gut-Brain axis to Alzheimer’s and neurodegenerative diseases needs extensive analysis. The various articles indicate that there are various questions with relevance...

03 August 2022 3,882 3 View

How to exctract difficult plasmids from bacteria?

Dear All, I have received the pLVX-M-puro plasmid in a bacterial stab and I can´t isolate it. I have tried several times, using fresh Amp-plates and Amp-LB media to first grow the single colonies...

13 April 2022 887 7 View

Is histopaque same as ficoll-paque? Can I use histopaque to isolate PBMC?

Peripheral blood mononuclear cells (PBMC)

01 February 2022 8,047 4 View

Culturing monocytes and monocyte-derived macrophages isolated from blood samples - How long do these cells last in culture?

Hi everyone, does anyone know the best condition to isolate and culture monocytes and monocyte-derived macrophages? Many papers suggest DMEM, others RPMI + 10% FBS. I am culturing these cells in...

12 November 2021 8,826 4 View

How can I calculate the theoretical molecular weight of my liposomes ?

I want to compare values obtained with the AF4 method I have, but first how can we easily assess the molecular weight ? I have 3 lipids. I assume, we multiply by their respective masses used...

28 September 2021 2,139 0 View

How to count nanoparticles?

I'm dealing with vesicles (not liposomes, so I dont know the composition and I can't do any assumption of their molarity based on their concentration and hydrodinamic radius), derived from...

17 September 2021 248 4 View

Why my lipid vesicles missing during extrusion?

Hi all, I have tried to do a calcein leakage assay by LUVs that I made (dopc:dopg 1:3, dopc:dopg 3:1, and dopc only). In doing so I dissolved my lipids film in 2 mL of 10mM tris buffer containing...

03 September 2021 6,210 1 View

Looking for an HPLC-UV software ?

I'm looking for a software able to process HPLC-UV chromatograms (espacially baseline correction and peak alignement) in order to integrate the data to statistical analysis.

25 May 2021 1,837 10 View

How to convert Waters raw file to mgf or mzML file format keeping the original charge state information?

Hi, I am using a Waters Select Series Cyclic IMS, and trying to convert MSe raw data (without using ionmobility) to another generic file format. I tried PLGS and MSconvert but they transform the...

29 April 2021 5,751 0 View

Questions about NK cells cultivation?

The NK cell culture method I chose is to add K562 and allogeneic PBMCs to the system, which have been treated with 100ug/ml MMC. Freshly isolated PBMCs are sorted by EasySep™ Human NK Cell...

14 April 2021 1,266 1 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

03 March 2021 1,693 3 View

Problem removing OG detergent from liposomes with Bio-beads?

Hello, I'm having a problem that I don't know how to solve. I am following a protocol that I did a year ago and now it does not work. I reconstitute the dry lipids (polar lipids from E.coli) in a...

10 February 2021 2,760 2 View

How to determine if a drc model is statistically fitting my data ?

Dear community, I am facing some issues and scientific questionings regarding the dose-response analysis using the drc package from R. Context : I want to know if two strains have different...

01 February 2021 8,225 1 View

Can you polarize Jurkat cells into different T helper subsets?

I am wondering if anyone has had success with in vitro polarization (via cytokines) of Jurkat cells. My goal is to understand the affect of a drug on Treg induction and differentiation. I...

13 January 2021 3,774 2 View

Cell number for 1mg protein?

We need to isolate 1mg protein, from ipsc-derived cortical neurons. About how many cells should I have, to get this protein amount from these cells?

18 December 2020 3,081 1 View

Resuspending cell pellet after ficoll centrifugation?

In my current project I am planning to isolate mononuclear cells from peritoneal ascites of cancer patients using ficoll centrifugation. However, following isolation of the mononuclear cell layer,...

27 October 2020 6,169 4 View

How to successfully produce agarose microwells with PDMS stamp?

My experiment aims to make spheroids by sacrificial micromolding technique. I am following the easy protocol: "...The PDMS stamps were deposited onto a 250 μl drop of 3% liquid agarose within the...

06 October 2020 3,833 3 View

Does anyone know the exact cleavage site of SaCas9?

Spcas9 has been reported to cleave ~ 3bp upstream of the start of the PAM as a blunt cut, Cpf1 has a staggered cut significantly upstream of the PAM, but I cant seem to find much information...

30 September 2020 9,066 2 View

Do the tannic acid-treated sheep RBCs show agglutination with FAdVs (fowl adenoviruses)?

As we know that Adenoviridae indirectly agglutinates the RBCs of chicken, mice, rhesus monkey, and human blood type "O".However, do the tannic acid-treated sheep RBC show agglutination with FAdVs...

18 September 2020 6,790 3 View

Why Salmonella makes TSB (Tryptic Soy Broth) medium get darker?

Hi everyone, we are making some experiments with Salmonella species in a microplate reader. We are basically characterizing its growth curve in 18 hour time of incubation. However, only one...

09 September 2020 2,821 5 View

Can anti-Fas (anti-CD95) be used to prepare apoptotic cancer cell as antigens for DC to present antigen?

Researchers usually used irradiation (such as x-ray) to induce cancer cell apoptosis to prepare available antigens for DC to uptake that present the antigen to T cells. Can other apoptotic methods...

27 August 2020 5,563 4 View

Does crosslinking actually increase or decrease the antigenicity of collagen as biomaterial?

Some studies point that crosslinking increase the inflammatory reaction of collagen[1-3]. while some other studies suggests that Cross-linking also can be introduced to reduce the antigenicity....

11 August 2020 3,393 3 View

Can anyone suggest a PhD dissertation area that encompass Microbiology and Immunology?

Can anyone please suggest some related topic or area in microbiology and immunology except COVID/SAARS?

05 July 2020 5,087 37 View

What's your expectation about the successful treatment of COVID-19 in the future, do you think will be immunologically or synthetically by drugs?

The clinical treatment of COVID-19 infected individuals.

25 June 2020 1,620 22 View

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