Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

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Why is electronic energy 0.000 for liquid crystal compounds and is invariable with temperature in Guassian 09 software?

Are there any suggestions or insights you can provide to help address this problem

06 August 2024 1,443 2 View

Provide a questionnaire for positive role of politics in nursing ?

I want to write article in a topic of positive role of politics in nursing, and I'm confused how can we add add questionnaire and what will be the questionnaires.

18 July 2024 10,021 3 View

How much sample we are required to do a pilot study for standardized an Achievement Test?

I am conducting a study on Effectiveness of e-content on academic achievement of secondary school science student.

17 July 2024 5,633 4 View

Does anyone know, any conference related to "mathematical data compression" topic?

Please suggest me, I want to present my research work.

13 July 2024 1,461 1 View

Issues in coating of cathode material on Al foil, why we are not getting proper coating and results when the cathode was coated on Al-foil..?

We are trying to coat some cathode materials on Al-foil ( ex - LiFePO4 , S@CNT, etc). When we coated the Al foil with the cathode (ex - S@CNT) material , the cycling result was annoying. But the...

09 July 2024 5,123 4 View

Taking a cut line through deck build in silvaco?

Hi everyone hope your all doing good. I’ve been looping my scripts and am trying to take a cut line of the conduction band energy with respect to y for each loop. Basically, I want to take a...

03 July 2024 9,134 1 View

My hippocampal mouse primary neurons clump when I plate them on microfluidic devices for axonal isolation. Any tip?

Hi all, I am working on both primary hippocampal mouse neurons and i3Neurons to isolate axonal material. I plate them in microfluidic devices in house made (like any other you can find on the...

01 July 2024 8,070 0 View

Please, what are the recent/top/free software for predicting data from MALDI-TOF that you have tried?

Please, can the fragmentation obtained from the mass spectrometry be predicted more easily using any free software? Kindly recommend it, thanks.

01 July 2024 5,327 6 View

Can we use MTCMOS instead of CMOS for designing SRAM Latch ?

Basically CMOS are used for designing the SRAM cells . How the functionality will differ if we use Multi threshold CMOS instead of CMOS generally MTcmos decrease the leakage power but it will...

28 June 2024 9,845 0 View

How to synthesize Dichloro(p-cymene)ruthenium(II) dimer in a good yield?

Suggest some procedure where i can synthesize [Ru(p-cymene)Cl2]2 in a good yield.

23 June 2024 9,244 2 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,157 4 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

CHO-K1 suspension adaptation protocol?

I am trying to adapt adherent CHO-K1 cells in F12K+10%FBS to suspension culture in only F12K, but in vain. I have followed Mirus bio as well as Thermo protocols, please suggest any alternative....

31 July 2024 3,756 0 View

How to make a Lateral flow control line more uniform?

We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...

31 July 2024 4,510 3 View

How to prevent hemolysis after performing cardiac puncture to draw blood from mice in serum?

We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?

29 July 2024 8,402 4 View

What is the weight of rotavirus A (ng)?

I am developing an ELISA kit for rotavirus A (G1P8 and G1P6). Does anyone know the weight (ng) of these two strains? Or to help me find their weight?

29 July 2024 3,138 2 View

Why my IgG antibody looks fragmented on non-reducing SDS-PAGE?

I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...

23 July 2024 6,664 6 View

What are the strategies to Enhance IgG-Producing Plasma Cells in mice for Monoclonal Antibody Development?

I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...

22 July 2024 9,160 2 View

What are those black/hollow structures?

Hello, I recently stained my cells with an Arf6 antibody. The healthy control looks pretty normal, while the disease group cells have abnormally large black structures in the cell body. Has anyone...

21 July 2024 1,044 1 View

How to activate 4E8 T cells with plate-bound anti-CD3 for a genome-wide CRISPR screen?

It was assumed that 4E8 T cells should be isolated and activated using plate-bound anti-human CD3, after which would be used for a genome-wide CRISPR screen. (Ref: Genome-wide CRISPR Screens in...

17 July 2024 3,371 0 View