Q&A for issues regarding electron microscopy | Contact experts in Electron Microscopy to get answers
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Questions related to Electron Microscopy
Hello I tried different approaches, but I cannot seem to get it right!and if anyone has SEM images of platelet rich plasma, I would appreciate of he/she could share so I can use as...
06 June 2015 5,825 1 View
Please, During the preparation of the fixative for electron microscopy I made a mistake, I forgot to dilute gluteraldehyde before adding it, I left my samples about an hour, then I catch and I...
06 June 2015 4,090 4 View
04 June 2015 8,903 3 View
I have carried out scanning Electron Microscope studies Thrips ( Name cannot be revealed at this moment). The characters are well studied in few reports but SEM studies not so far.Along with SEM...
05 May 2015 8,291 2 View
Hi, I am looking for suitable paramteres for the ion milling process with PIPSII (GATAN). The CrN, TiN, TiAlN coatings are to be prepared for the TEM investigations. A hint would be really...
05 May 2015 8,180 3 View
Hi everyone, I am trying to identify vesicles at different stages in the autophagic pathway in the heart, especially autophagosomes. However, the EM quality seems good, but I can rarely see...
27 March 2015 531 5 View
I am surveying the ultrastructure of the endothelial cell layer of mouse aortic rings using a fairly standard electron microscopy protocol to prepare my samples (fixation in 2.5% glutaraldehyde...
10 March 2015 973 8 View
I'm trying to analyse E. coli with TEM, attached file is what I've done, the control sample without any treatment. It shows empty part in the cells. I've tried 1.changing fixatives' concentration...
03 March 2015 1,591 11 View
03 March 2015 860 6 View
Electron microscopy Nanoparticles
03 March 2015 2,664 6 View
As I am doing the experimentation on preparation of nanoemulsion (oil in water) and we have SEM and TEM analytical techniques So can I use these techniques for droplet size measurement.
03 March 2015 9,480 6 View
I want an alternative to Osmium tetroxide or sodium caucodylate.
23 February 2015 323 6 View
02 February 2015 2,910 4 View
I have searched briefly but I haven't found any instances where light microscopy has been used on isolated mitochondria. I found many examples where electron microscopy or electron tomography has...
02 February 2015 3,206 6 View
For my experience, every time I prepare a TEM lamella from a bulk Al sample it starts bending when its thickness is below 100 nm. It cannot be charging because there is electrical continuity...
01 January 2015 8,554 11 View
I isolate exosomes and I'm going to carry out electron microscopy in the next days, so I want to know what is the best condition to store in order to avoid rupture due to drastic thaw-freeze...
01 January 2015 9,737 9 View
In order to study ROS and mitochondria morphology I require to study these things Thank you guys in advance.
01 January 2015 5,106 5 View
I synthesized a specific fluorophore probe. It can bind with a specific site on DNA, but the problem is maybe it can also bind with RNA. So I need to show that this probe results in only DNA, not...
01 January 2015 9,612 6 View
Dear All, We thought to prepare proliposomes to be formulated as a tablet using lecithin. Please let me know how to check whether the liposomes are produced invitro using microscopic technique /...
12 December 2014 9,929 3 View
Kindly let me know which formula or which concept will use to solve the question. This question belongs to the Electron microscopy of samples
12 December 2014 8,920 5 View
In transmission electron microscopy (TEM) irradiation damage due to knock-on processes (cascade displacements) of atoms is a major concern. For carbon atoms, most textbooks estimate this to be...
12 December 2014 2,845 2 View
I am working on electron microscopic study of NADPH-diaphorase in neuron. But I am not good at identifying the ultrastructural image.
11 November 2014 7,919 3 View
please how do I measure the density, size, viability and thickness of transmission electron microscopy micrographs and what tools can be used? In a case where I have three micrographs that is been...
11 November 2014 5,640 1 View
We are planing to take fish intestine samples for electron microscopy. As this is our first experience, I would like to know how long the samples can be kept in the glutaraldehyde solution.
11 November 2014 5,364 9 View