Q&A for issues regarding electron microscopy | Contact experts in Electron Microscopy to get answers
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Questions related to Electron Microscopy
I have been trying to process neuronal cells grown as a monolayer on glass coverslips for standard TEM but my images are not to the standard I was hoping for (see attached). It looks to me like...
28 October 2015 3,702 9 View
I am depositing SiOx films using HMDSO / Oxygen gas mixture by low pressure RF PECVD method. In FTIR spectra I get two overlapped peaks around 1050 cm-1 and 1135 cm-1. Does this have any relation...
23 October 2015 3,914 8 View
10 Hp, VFDs
23 September 2015 2,046 0 View
Can anyone throw some light to understand the degree of quantitative data that can be generated by employing (SEM)-EDS run, as compared to other quantitative technique? Let us assume the situation...
11 September 2015 3,410 4 View
In electron microscope fixation does they have the same effect?
08 August 2015 5,974 5 View
Characterization - Transmission Electron Microscopy What is the method to calculate d-value from SAD pattern rings ?
26 July 2015 3,916 2 View
07 July 2015 9,028 3 View
I´m trying to perform single particle reconstruction (SPR) of a protein of mine (around 190 kDa) using negative stain data. Normally, particles under negative stain look relatively well but this...
07 July 2015 2,235 2 View
Hi all, I would like to do preembedding immunogold electron microscopy on muscle. Do anyone have a protocol? Thank you very much!!!
07 July 2015 7,474 0 View
We have southbay model 515 dimpler but screw that push towards to the brass disc is missing. does anyone can measure or know the length, diameter and thread size of the screw? Is it metric or...
07 July 2015 7,340 0 View
Electron Microscopy expert We have spotted our lipopeptide sample on glow discharged carbon coated copper grid (1 min) and used freshly prepared 2 % uranyl acetate (2 min). But TEM images are...
07 July 2015 1,602 4 View
I did immunoelectronmicroscopy silverstaing on cryosections of bovine retina tissue. I found a dark black background after staining on the slides along with the tissue. Is it a common phenomenon...
07 July 2015 9,768 3 View
I am finding a picture of hematopoietic stem cell for my presentation but I'm not sure if this is HSC or not.
27 June 2015 1,831 3 View
I'm trying to prepare electron microscopy grids with incubate specimen with Goldnano conjugated aptamer. Has anyone try this? Give me some tips for conjugation. The size of Goldnano going to use,...
06 June 2015 4,443 5 View
06 June 2015 6,526 3 View
Hello I tried different approaches, but I cannot seem to get it right!and if anyone has SEM images of platelet rich plasma, I would appreciate of he/she could share so I can use as...
06 June 2015 5,835 1 View
Please, During the preparation of the fixative for electron microscopy I made a mistake, I forgot to dilute gluteraldehyde before adding it, I left my samples about an hour, then I catch and I...
06 June 2015 4,101 4 View
04 June 2015 8,920 3 View
Hello Every Body, I am building up an electronic system, that includes an electronic module along with an interfacing unit (Arduino or Pi). I am trying to protect my system from any abrupt power...
11 May 2015 8,441 3 View
I have carried out scanning Electron Microscope studies Thrips ( Name cannot be revealed at this moment). The characters are well studied in few reports but SEM studies not so far.Along with SEM...
05 May 2015 8,311 2 View
Hi, I am looking for suitable paramteres for the ion milling process with PIPSII (GATAN). The CrN, TiN, TiAlN coatings are to be prepared for the TEM investigations. A hint would be really...
05 May 2015 8,197 3 View
Hi everyone, I am trying to identify vesicles at different stages in the autophagic pathway in the heart, especially autophagosomes. However, the EM quality seems good, but I can rarely see...
27 March 2015 541 5 View
I am surveying the ultrastructure of the endothelial cell layer of mouse aortic rings using a fairly standard electron microscopy protocol to prepare my samples (fixation in 2.5% glutaraldehyde...
10 March 2015 982 8 View
I'm trying to analyse E. coli with TEM, attached file is what I've done, the control sample without any treatment. It shows empty part in the cells. I've tried 1.changing fixatives' concentration...
03 March 2015 1,598 11 View