Q&A for issues regarding electron microscopy | Contact experts in Electron Microscopy to get answers
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Questions related to Electron Microscopy
can we identify theta prime and theta double prime phase from SAED pattern and how?
12 December 2016 4,422 2 View
07 December 2016 3,291 2 View
I´m wondering how common is the use of the morphometric parameter "Mitochondrial area" in biomedicine applied ultrastructural studies with transmission electron microscopy.
11 November 2016 9,922 0 View
We are facing problems with tissue preservation. We have both mechanical damage in the tissue may be resulting from handling and broken cell membranes indicating unproperly set osmolarity in the...
11 November 2016 2,519 5 View
Dear all, I used carbon coated copper grid 200 mesh for my sample (gold nanoclusters dispersed in ethanol) to get images. Sometimes I got different types of ring-shaped damage after few...
11 November 2016 542 10 View
In percentage of crystal-sample distance and/or working distance
10 October 2016 10,042 1 View
Hi I would want try EM (negative stain method) with my sample but in size exclusion column I identify 3 different oligomers. Can I do EM? or I need a homogeneous sample. Thank you
10 October 2016 6,495 3 View
10 October 2016 1,241 3 View
In SEM, it is written that the Number of secondary electrons can be greater than the number of incoming (primary) electrons. Again, I read in various sources that the Maximum yield (No. of emitted...
27 September 2016 1,485 4 View
Osmium Tetroxide is traditionally used in electron microscopy both as a fixative and a heavy metal stain. But it too expensive, toxic and volatile.
26 September 2016 9,505 4 View
In the wet transfer method (pmma assisted method), after using the TEM grid to fish up the PMMA film with MoS2, we need to dissolve the PMMA film. Following a lot of literature, I use acetone to...
20 September 2016 9,814 6 View
09 September 2016 4,285 5 View
09 September 2016 3,754 5 View
09 September 2016 10,121 6 View
I am using 1% Ga cacodylate-filtered buffer for quick hard fix of my phage sample to grid then perform negative staining by 2% uranyl acetate. But the phage picture look like empty. Does it mean...
30 August 2016 3,141 4 View
Lately, I would like to take SEM or TEM image for magnetic Fe3O4 nanoparticles. (about 50~60 nm) The particles are modified with peptide sequences and immersed in PBS buffer. To prepare the sample...
08 August 2016 5,346 10 View
08 August 2016 8,343 5 View
Currently I use the method of submerging the grid below the water surface and coaxing sections onto grid with hair-tools. The problem is that they often drift away and do not settle onto the...
08 August 2016 9,258 9 View
I am using ImageJ but it is not giving me clear images. I want to get crystal clear images free from the noise. I don't know how to use filters to increase the quality of my image. Can anyone...
08 August 2016 1,536 18 View
Thank you for reading my question. I'm looking at a TEM-grid without C-component since I'd like to get a microscopic image showing C-layers over metal oxide. When I try to differentiate C layer...
23 June 2016 3,654 6 View
Our TEM is JEM 2100 with EELS Gatan 963 with GMS3 software. For the quantification we have to feed the collectinn angle and convergence angle values.
23 June 2016 5,461 4 View
how to attach particles to carbon film on grid for EM imaging? We tried poly lysine,but it will contribute contrast for EM imaging
06 June 2016 3,345 4 View
We found by electron microscopy ultrastructural defects in the muscle of a KO mice. As the lacZ gene was inserted during KO, we are concerned by the possibility that lacZ expression itself causes...
06 June 2016 7,040 0 View
06 June 2016 9,517 4 View