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Questions related to Electron Microscopy
In SEM, it is written that the Number of secondary electrons can be greater than the number of incoming (primary) electrons. Again, I read in various sources that the Maximum yield (No. of emitted...
09 September 2016 3,736 5 View
In the wet transfer method (pmma assisted method), after using the TEM grid to fish up the PMMA film with MoS2, we need to dissolve the PMMA film. Following a lot of literature, I use acetone to...
09 September 2016 10,104 6 View
I am using 1% Ga cacodylate-filtered buffer for quick hard fix of my phage sample to grid then perform negative staining by 2% uranyl acetate. But the phage picture look like empty. Does it mean...
30 August 2016 3,126 4 View
Lately, I would like to take SEM or TEM image for magnetic Fe3O4 nanoparticles. (about 50~60 nm) The particles are modified with peptide sequences and immersed in PBS buffer. To prepare the sample...
08 August 2016 5,331 10 View
08 August 2016 8,326 5 View
Currently I use the method of submerging the grid below the water surface and coaxing sections onto grid with hair-tools. The problem is that they often drift away and do not settle onto the...
08 August 2016 9,237 9 View
I am using ImageJ but it is not giving me clear images. I want to get crystal clear images free from the noise. I don't know how to use filters to increase the quality of my image. Can anyone...
08 August 2016 1,523 18 View
Thank you for reading my question. I'm looking at a TEM-grid without C-component since I'd like to get a microscopic image showing C-layers over metal oxide. When I try to differentiate C layer...
23 June 2016 3,640 6 View
Our TEM is JEM 2100 with EELS Gatan 963 with GMS3 software. For the quantification we have to feed the collectinn angle and convergence angle values.
23 June 2016 5,448 4 View
how to attach particles to carbon film on grid for EM imaging? We tried poly lysine,but it will contribute contrast for EM imaging
06 June 2016 3,331 4 View
We found by electron microscopy ultrastructural defects in the muscle of a KO mice. As the lacZ gene was inserted during KO, we are concerned by the possibility that lacZ expression itself causes...
06 June 2016 7,022 0 View
06 June 2016 9,501 4 View
06 June 2016 595 6 View
We made adaptations on several methodologies and their combinations allowed wonderful photos of bacteriophages in TEM (Transmission Electron Microscopy) and we want publish these adaptations with...
06 June 2016 6,339 2 View
I am studying the morphology of monolayer cells using TEM, but i wonder whether ultrastructure of cells attached can be viewed when they attached on scaffold .Can anyone suggest the methodology...
05 May 2016 10,082 2 View
1.what parameters determine the coherency between precipitate and matrix 2. How to identity coherent precipitates using transmission electron microscopy
05 May 2016 10,056 0 View
Hi all, I am looking to buy/download the following book: "Hayat MA. 2000. Principles and techniques of electron microscopy", but I did not see any seller in Italy. I will appreciate if someone...
05 May 2016 5,762 3 View
I am using exosome immunoprecipitation reagent from Thermofisher, and I would like to find an elution buffer compatible with TEM. Thermo's protocol calls for elution with Glycine HCl, but I have...
05 May 2016 2,905 1 View
What is the information obtained from SAED pattern and Convergent beam electron diffraction (CBED) of nanomaterials? is both gives same information or differnt?
22 April 2016 6,045 3 View
By the term Kikuchi bands I mean bright band bordered by two dark lines (typical for EBSD as well), and by term Kikuchi lines the band bordered by excess and deficient line. Both are possible to...
18 April 2016 9,663 9 View
Dear RGs I wonder if the German manufactor of ultra microtome knives dEYEmond is still alive? The website of this firm and it's 'mother' SciMed is not fully functional, no responce to mail and...
04 April 2016 8,551 1 View
different angular shapes represent which quality of material?
04 April 2016 7,876 1 View
04 April 2016 8,477 3 View
We have recently taken some super-resolved images of live CHO-K1 cells expressing eGFP-tubulin. Some of the microtubule strands appear in the super-resolved image to be actually two tubules...
10 March 2016 3,990 2 View