Please,
During the preparation of the fixative for electron microscopy I made a mistake, I forgot to dilute gluteraldehyde before adding it, I left my samples about an hour, then I catch and I changed the fixation medium . Is what I've done good or my attachment is missed , thank you in advance
Aat Mulder
hi Nadia, unfortunately when you go from a high concentration of glutaraldehyde to a lower, it will have no effect anymore, certainly not for glutaraldehyde as this is a irreversible fixative. The other way round can be sometimes ( from a low concentration to a higher concentration) useful. Was it for morphology? due to the high concentration there might be artefacts in your tissue.
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