Hi all, I would like to do preembedding immunogold electron microscopy on muscle. Do anyone have a protocol? Thank you very much!!!
Hi all, I'm trying to do flat embedding of monolayer cell culture in LRWhite, but the results are not exciting. I used the "PTFE flat embedding mold" from Ted Pella (attached there is a picture of...
08 September 2018 3,204 0 View
Hi guys, I will appreciate if somebody could give me some suggestions on how to perform transmission electron microscopy on structures which have been grown inside a chip like...
04 May 2018 9,838 3 View
Hi guys, I have some ampules of formaldehyde, 16%, methanol free, ultra pure (Polysciences cat. number 18814-10) and I would like to use them for transmission electron microscopy with...
03 April 2018 4,615 8 View
Hi guys, I am doing pre-embedding immunogold electron microscopy. Unfortunately I don't have "cold water fish skin gelatin" as component of blocking agent (along with BSA and NGS). I would like to...
08 September 2017 2,000 0 View
Hi guys, I would like to do preembedding immunogold on cell cultures. I followed different protocols and I tried to localize different proteins, with both Nanogold (1.4nm) and Aurion (0.8nm) gold...
06 July 2017 3,909 10 View
Hi guys, I was observing some microglial primary cultured cells with the transmission electron microscope and I found some structures I never seen. I really appreciate if someone could provide to...
03 April 2017 3,945 15 View
Dear all, A lab asked to our electron microscopy facility to analyze whether antidepressants change autophagy in mouse hippocampus neurons with transmission electron microscopy. In order to do...
03 April 2017 2,900 7 View
Hi guys, I would like to know how to properly use the Pefect Loop in order to pick up ultrathin sections and release them well oriented onto a formvar slot grid. Thank you very much!!! Francesco
31 December 2016 9,854 6 View
Hi, I will appreciate if someone could give me a detailed protocol for fixation adn embedding adipose tissue for transmission electron microscopy, in order to see mitochondria cristae. Thank you...
08 September 2016 8,451 3 View
Hi all, I am using home made formvar coated slot grids, without carbon. I make them in advance from 0.5% formvar in chloroform and then I store them inside a Petri dish. I am encoutering problems...
05 June 2016 4,142 2 View
I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?
11 August 2024 3,115 1 View
If we map as a continuous motion an ionising electron (beginning its journey at n=1) in an H atom, a specific hyperbolic spiral appears (see animation). When we solve this spiral formula, we find...
07 August 2024 5,343 2 View
Hello dear colleagues, We have prepared a manuscript on NiTi-based alloys and are seeking a second opinion on our current TEM results. If you are a Ph.D. holder with experience in TEM and have...
07 August 2024 9,563 0 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
average particle size calculation from TEM
04 August 2024 2,921 1 View
If from a geometric perspective the non-halogens, non-noble gases have more empty spots in their valence shell, and the filling/exiting of any of the empty spots in the shell constitutes a...
03 August 2024 4,787 2 View
Why is the molecule's orientation with an electric field affect polarizability? Electrons are diffuse enough to be independent with respect to orientation and effect of electric field on...
03 August 2024 7,843 1 View
I'm guessing it's because the ligand experiences too much electron repulsion or proton repulsion from the chromium to insert them close to the 3d-orbitals which are close to the metal nucleus. Is...
03 August 2024 1,370 1 View
I am working on a SEM model where i have 7 latent variables (6 exogenous and 1 endogenous). In AMOS when I co-vary the exogenous constructs, only 2 paths are coming significant out of 6. But when...
03 August 2024 6,028 4 View