Q&A for issues regarding electron microscopy | Contact experts in Electron Microscopy to get answers
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Questions related to Electron Microscopy
protocols of electron microscopy and other methods????
03 March 2016 3,263 3 View
How should I inactivate my adenoviruses for transmission electron microscopy (negative staining)? Which methods affect their visible structure the least?
03 March 2016 1,692 4 View
Dear collegues! In all protocols, the bone at first fixed in 4% formaldehyde. But nowhere is it written that is it fixation in buffer solution (phosphate, cacodylate) or in distilled water. The...
03 March 2016 854 11 View
We have recently taken some super-resolved images of live CHO-K1 cells expressing eGFP-tubulin. Some of the microtubule strands appear in the super-resolved image to be actually two tubules...
03 March 2016 2,701 2 View
Hi everyone, I'm new to this field. Can someone explain to me in layperson's language why the thickness of a biological sample is important when doing cryo-electron microscopy? I'm trying to image...
03 March 2016 8,721 6 View
02 March 2016 3,219 10 View
Some protocol mentioned the saline perfusion for mouse brain to flush out blood is very short, say less than 2 minutes for detection of synaptic changes for electron microscopy. They say it...
29 February 2016 1,533 3 View
I'm preparing the samples for my experiment for TEM. I did the resin embedding step. After adding the pure resin I centrifuged the cells at 1,500 rpm 5 minutes but could't pellet it well. The...
23 February 2016 7,923 3 View
High-resolution transmission electron microscopy (HRTEM) or (HREM) is an imaging mode of the transmission electron microscope (TEM)
08 February 2016 8,257 4 View
I am trying to observe autophagosome in the starved MEFs cell by TEM (600,000x), but I have never seen the double-membrane of autophagosomes. I only saw a lot of single-membrane vacuoles. Look...
02 February 2016 10,024 7 View
02 February 2016 7,458 5 View
I want to leave the samples for electron microscopy in gluteraldehyde fixative over the weekend and continue the process next week. Will it affect the samples? How long can the samples be left in...
02 February 2016 9,824 6 View
02 February 2016 949 4 View
02 February 2016 1,666 3 View
How to calculate the grain size of following images.
30 January 2016 6,757 6 View
Hi everyone, I'm planning on looking at some electron microscopy pictures of axons to calculate the g-ratio. However, the axons are not very regular in shape, as in the oval shapes creates a...
20 January 2016 7,303 0 View
I'm trying to find out the best way to stain epibiotic ciliates. Most articles have used Protargol (silver proteinate), however some mention hematoxylin. Is there any difference in the way they...
12 January 2016 718 2 View
I have prepared up to dehydration of bacterial sample in 30, 50, 70, 90 and 100% graded ethanol dilutions and washed each three times.
03 January 2016 1,881 6 View
The size obtained from TEM of Carbon Dots are the size of carbon core alone or it includes the length of surface functionalization too?
01 January 2016 8,314 4 View
01 January 2016 4,653 0 View
01 January 2016 925 2 View
This SAED Pattern was taken by Cs-Corrected Scanning Transmission Electron Microscopy?
17 December 2015 1,115 6 View
I am a teacher of university studying the ore microscopy, and is anyone can recommend a classic guidebook or website to present the beautiful and explain these photomicrographs?
12 December 2015 7,407 6 View
Any optimised approaches to apply to general procedures. Does anyone have tips/recommendations for fixation composition [e.g. additives to Karnovsky's] or embedding etc. of mouse sciatic nerve for...
12 December 2015 7,768 5 View