Q&A for issues regarding electron microscopy | Contact experts in Electron Microscopy to get answers
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Questions related to Electron Microscopy
Thank you for reading my question. I'm looking at a TEM-grid without C-component since I'd like to get a microscopic image showing C-layers over metal oxide. When I try to differentiate C layer...
06 June 2016 609 6 View
We made adaptations on several methodologies and their combinations allowed wonderful photos of bacteriophages in TEM (Transmission Electron Microscopy) and we want publish these adaptations with...
06 June 2016 6,351 2 View
I am studying the morphology of monolayer cells using TEM, but i wonder whether ultrastructure of cells attached can be viewed when they attached on scaffold .Can anyone suggest the methodology...
05 May 2016 10,100 2 View
1.what parameters determine the coherency between precipitate and matrix 2. How to identity coherent precipitates using transmission electron microscopy
05 May 2016 10,071 0 View
Hi all, I am looking to buy/download the following book: "Hayat MA. 2000. Principles and techniques of electron microscopy", but I did not see any seller in Italy. I will appreciate if someone...
05 May 2016 5,783 3 View
I am using exosome immunoprecipitation reagent from Thermofisher, and I would like to find an elution buffer compatible with TEM. Thermo's protocol calls for elution with Glycine HCl, but I have...
05 May 2016 2,919 1 View
What is the information obtained from SAED pattern and Convergent beam electron diffraction (CBED) of nanomaterials? is both gives same information or differnt?
22 April 2016 6,069 3 View
By the term Kikuchi bands I mean bright band bordered by two dark lines (typical for EBSD as well), and by term Kikuchi lines the band bordered by excess and deficient line. Both are possible to...
18 April 2016 9,681 9 View
Dear RGs I wonder if the German manufactor of ultra microtome knives dEYEmond is still alive? The website of this firm and it's 'mother' SciMed is not fully functional, no responce to mail and...
04 April 2016 8,568 1 View
different angular shapes represent which quality of material?
04 April 2016 7,896 1 View
04 April 2016 8,492 3 View
Can aynone, shear with me or give me any indication about the resultat (quantity of viral copies) that I should find in Hep G2 or Huh 7.5 cells infected with a 0.1 MOI of HBV, Taking that the...
18 March 2016 9,836 2 View
We have recently taken some super-resolved images of live CHO-K1 cells expressing eGFP-tubulin. Some of the microtubule strands appear in the super-resolved image to be actually two tubules...
10 March 2016 4,013 2 View
protocols of electron microscopy and other methods????
03 March 2016 3,275 3 View
How should I inactivate my adenoviruses for transmission electron microscopy (negative staining)? Which methods affect their visible structure the least?
03 March 2016 1,707 4 View
Dear collegues! In all protocols, the bone at first fixed in 4% formaldehyde. But nowhere is it written that is it fixation in buffer solution (phosphate, cacodylate) or in distilled water. The...
03 March 2016 873 11 View
03 March 2016 2,718 2 View
Hi everyone, I'm new to this field. Can someone explain to me in layperson's language why the thickness of a biological sample is important when doing cryo-electron microscopy? I'm trying to image...
03 March 2016 8,735 6 View
02 March 2016 3,239 10 View
Some protocol mentioned the saline perfusion for mouse brain to flush out blood is very short, say less than 2 minutes for detection of synaptic changes for electron microscopy. They say it...
29 February 2016 1,549 3 View
I'm preparing the samples for my experiment for TEM. I did the resin embedding step. After adding the pure resin I centrifuged the cells at 1,500 rpm 5 minutes but could't pellet it well. The...
23 February 2016 7,946 3 View
High-resolution transmission electron microscopy (HRTEM) or (HREM) is an imaging mode of the transmission electron microscope (TEM)
08 February 2016 8,277 4 View
I am trying to observe autophagosome in the starved MEFs cell by TEM (600,000x), but I have never seen the double-membrane of autophagosomes. I only saw a lot of single-membrane vacuoles. Look...
02 February 2016 10,038 7 View
02 February 2016 7,479 5 View