Q&A for issues regarding electron microscopy | Contact experts in Electron Microscopy to get answers
1,621 views 396 posts
Questions related to Electron Microscopy
Any optimised approaches to apply to general procedures. Does anyone have tips/recommendations for fixation composition [e.g. additives to Karnovsky's] or embedding etc. of mouse sciatic nerve for...
02 December 2015 390 4 View
Hi all, I would like to batch convert simultaneously pictures taken with TIA in a FEI Tecnai electron microscope from ".emi" to ".TIFF" format, instead of doing it one by one. I will appreciate if...
27 November 2015 3,874 5 View
I was preparing some negative stain EM grids for protein samples. I purified the proteins with gel filtration right before grid preparation and the traces looked very good (quite symmetric and...
17 November 2015 4,664 11 View
I find that we can fix cells and tissues in 0.1M Sodium cacodylate containing 4% formalin and 2.5% glutaraldehyde, Can i use this for microcycst of echinococcus in liquid culture medium too? (M199...
11 November 2015 4,042 9 View
11 November 2015 3,465 5 View
Using ImageJ, what is the best way to improve the depth of field of bright-field z-stack images? I tried the EDF plugin with poor results.
11 November 2015 10,069 11 View
11 November 2015 563 12 View
I am having the full-length homotrimer coat protein's predicted model. I want to rebuild virion capsid (Icosahedral virion) by using this homotrimer. Capsid structure of this virion is available...
11 November 2015 9,299 3 View
In particular studying rearrangement of the protein that encapsulate the virus.
11 November 2015 3,636 4 View
02 November 2015 4,648 8 View
I have been trying to process neuronal cells grown as a monolayer on glass coverslips for standard TEM but my images are not to the standard I was hoping for (see attached). It looks to me like...
28 October 2015 3,689 9 View
I am depositing SiOx films using HMDSO / Oxygen gas mixture by low pressure RF PECVD method. In FTIR spectra I get two overlapped peaks around 1050 cm-1 and 1135 cm-1. Does this have any relation...
23 October 2015 3,893 8 View
Can anyone throw some light to understand the degree of quantitative data that can be generated by employing (SEM)-EDS run, as compared to other quantitative technique? Let us assume the situation...
11 September 2015 3,389 4 View
In electron microscope fixation does they have the same effect?
08 August 2015 5,961 5 View
Characterization - Transmission Electron Microscopy What is the method to calculate d-value from SAD pattern rings ?
26 July 2015 3,906 2 View
07 July 2015 9,018 3 View
I´m trying to perform single particle reconstruction (SPR) of a protein of mine (around 190 kDa) using negative stain data. Normally, particles under negative stain look relatively well but this...
07 July 2015 2,219 2 View
Hi all, I would like to do preembedding immunogold electron microscopy on muscle. Do anyone have a protocol? Thank you very much!!!
07 July 2015 7,462 0 View
We have southbay model 515 dimpler but screw that push towards to the brass disc is missing. does anyone can measure or know the length, diameter and thread size of the screw? Is it metric or...
07 July 2015 7,332 0 View
Electron Microscopy expert We have spotted our lipopeptide sample on glow discharged carbon coated copper grid (1 min) and used freshly prepared 2 % uranyl acetate (2 min). But TEM images are...
07 July 2015 1,582 4 View
I did immunoelectronmicroscopy silverstaing on cryosections of bovine retina tissue. I found a dark black background after staining on the slides along with the tissue. Is it a common phenomenon...
07 July 2015 9,757 3 View
I am finding a picture of hematopoietic stem cell for my presentation but I'm not sure if this is HSC or not.
27 June 2015 1,824 3 View
I'm trying to prepare electron microscopy grids with incubate specimen with Goldnano conjugated aptamer. Has anyone try this? Give me some tips for conjugation. The size of Goldnano going to use,...
06 June 2015 4,433 5 View
06 June 2015 6,519 3 View