Q&A for issues regarding electron microscopy | Contact experts in Electron Microscopy to get answers
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Questions related to Electron Microscopy
I want to leave the samples for electron microscopy in gluteraldehyde fixative over the weekend and continue the process next week. Will it affect the samples? How long can the samples be left in...
02 February 2016 9,840 6 View
I'm preparing the samples for my experiment for TEM. I did the resin embedding step. After adding the pure resin I centrifuged the cells at 1,500 rpm 5 minutes but could't pellet it well. The...
02 February 2016 965 4 View
Some protocol mentioned the saline perfusion for mouse brain to flush out blood is very short, say less than 2 minutes for detection of synaptic changes for electron microscopy. They say it...
02 February 2016 1,685 3 View
How to calculate the grain size of following images.
30 January 2016 6,773 6 View
Hi everyone, I'm planning on looking at some electron microscopy pictures of axons to calculate the g-ratio. However, the axons are not very regular in shape, as in the oval shapes creates a...
20 January 2016 7,313 0 View
I'm trying to find out the best way to stain epibiotic ciliates. Most articles have used Protargol (silver proteinate), however some mention hematoxylin. Is there any difference in the way they...
12 January 2016 736 2 View
I need to genotype single 27hpf zebrafish embryos that have already undergone WISH but I'm not sure how is best to extract the DNA given that they have previously been fixed in 4% PFA and are now...
11 January 2016 1,475 3 View
I have prepared up to dehydration of bacterial sample in 30, 50, 70, 90 and 100% graded ethanol dilutions and washed each three times.
03 January 2016 1,893 6 View
The size obtained from TEM of Carbon Dots are the size of carbon core alone or it includes the length of surface functionalization too?
01 January 2016 8,329 4 View
01 January 2016 4,664 0 View
01 January 2016 935 2 View
This SAED Pattern was taken by Cs-Corrected Scanning Transmission Electron Microscopy?
17 December 2015 1,135 6 View
I am a teacher of university studying the ore microscopy, and is anyone can recommend a classic guidebook or website to present the beautiful and explain these photomicrographs?
12 December 2015 7,421 6 View
Any optimised approaches to apply to general procedures. Does anyone have tips/recommendations for fixation composition [e.g. additives to Karnovsky's] or embedding etc. of mouse sciatic nerve for...
12 December 2015 7,781 5 View
02 December 2015 414 4 View
Hi all, I would like to batch convert simultaneously pictures taken with TIA in a FEI Tecnai electron microscope from ".emi" to ".TIFF" format, instead of doing it one by one. I will appreciate if...
27 November 2015 3,892 5 View
I was preparing some negative stain EM grids for protein samples. I purified the proteins with gel filtration right before grid preparation and the traces looked very good (quite symmetric and...
17 November 2015 4,691 11 View
I find that we can fix cells and tissues in 0.1M Sodium cacodylate containing 4% formalin and 2.5% glutaraldehyde, Can i use this for microcycst of echinococcus in liquid culture medium too? (M199...
11 November 2015 4,063 9 View
11 November 2015 3,480 5 View
Using ImageJ, what is the best way to improve the depth of field of bright-field z-stack images? I tried the EDF plugin with poor results.
11 November 2015 10,089 11 View
11 November 2015 578 12 View
I am having the full-length homotrimer coat protein's predicted model. I want to rebuild virion capsid (Icosahedral virion) by using this homotrimer. Capsid structure of this virion is available...
11 November 2015 9,316 3 View
In particular studying rearrangement of the protein that encapsulate the virus.
11 November 2015 3,648 4 View
02 November 2015 4,660 8 View