Q&A for issues regarding electron microscopy | Contact experts in Electron Microscopy to get answers
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Questions related to Electron Microscopy
The infectious viruses such as Hepatitis B, C and HIV are endemic in some developing countries. A number of people practice "shaolin" haircut [clean hair shave]. a number of times people...
03 December 2018 8,540 5 View
Hi everybody. I wonder if there is someone here that has successfully worked with TEM grids sold by Electron Microscopy Sciences for the analysis of extracellular vesicles (microvesicles and...
14 November 2018 10,114 1 View
11 November 2018 9,637 1 View
Hello, congratulations for this great project! I'm curious about the portability of this tactile display, specifically the power supply/consumption. I've read it uses Li-ion batteries and it...
05 November 2018 8,610 1 View
The experiment is very simple. It consists of two parallel spark gaps, one spark gap is approximately 0,5 mm wide and the second gap is a little bit wider, about 0,7 mm wide. The parallel distance...
19 October 2018 2,584 4 View
For sample preparation for electron microscopy do I need to prewash with saline and then with the fixative? Or do I need to to leave out the prewash ?
10 October 2018 7,806 0 View
Hey everyone, We have been trying to image some amyloid fibrils under STEM using 2% UAR uranyl acetate replacement stain but we haven't been consistently getting good images. Most of our grids...
10 October 2018 5,040 5 View
Dear colleagues, Did you use HPF for some super-soft tissue like testis? Operation with "sampled" specimens like pieces of tissue, small worms, etc. is pretty clear. But when we have some...
10 October 2018 7,593 15 View
Hi all, I made an error in my recipe for making a PHEM buffer solution and realised that I put 10 x more HEPES into the PHEM buffer than I should have. The working concentration of HEPES in 1 x...
31 July 2018 2,476 8 View
which one is excellent result oriented protocol for fixation of Zebrafish , Danio rerio tissues, for ultramicrotomy on Transmission electron microscopy?
07 July 2018 4,676 0 View
We cannot identify this structure, EM of Drosophila larvae near the NMJ. Does anybody have any ideas?
07 July 2018 5,780 1 View
Dear colleagues, Preparation of blocks for FIB-SEM tomography is a little tricky process. Which design do you prefer and which technique and tricks use in the preparation? I make the blocks...
30 June 2018 518 1 View
Dear colleagues, We have prepared a few 6 mm sapphire discs with cells monolayers by HPF, they are in liquid nitrogen now. These disks are too big for conventional Leica freeze substitution...
20 June 2018 8,512 7 View
Recently, I read a paper about scanning ultrafast electron microscopy (SUEM) ( Nano Lett. 2017, 17, 3675−3680). Using this method, the author can directly image the dynamics of photoexcited...
07 June 2018 7,938 1 View
My background is chemistry and now encounter question in semiconductor physics. I use Polyethylene oxide (PEO) to modify heavily doped silicon. Based on UPS data, I find that the the work...
07 June 2018 2,252 8 View
06 June 2018 827 1 View
I have started to work with isolation of exosomes and I used the protocol from this paper (ultracentrifuge): "Isolation and characterization of exosomes from cell culture supernatants and...
06 June 2018 8,986 2 View
I'm new to TEM, I have some difficulties in mitochondrial identification. I am not sure which is mitochondria. Can anyone help me?
06 June 2018 3,902 0 View
06 June 2018 8,110 8 View
The tissue was embbeded in Spurr Resin. The infiltration process was 1:3, 1:2, 1:1, 2:1, Pure (Resin x Acetone). When I went to make the cut, I coudn't cut nothing. Is there any protocol to...
25 May 2018 7,947 2 View
Dear fellow researchers, does anyone have an idea about activity of typical standards for Th (ThO2 - Thorium oxide) and U (UO2 - uranite) used for EPMA/WDS? What are your experience? Is it above...
05 May 2018 569 3 View
Hi guys, I will appreciate if somebody could give me some suggestions on how to perform transmission electron microscopy on structures which have been grown inside a chip like...
05 May 2018 9,784 3 View
05 May 2018 7,581 3 View
what the difference between them in Gold nanoparticles characterization?
05 May 2018 5,309 3 View