Q&A for issues regarding electron microscopy | Contact experts in Electron Microscopy to get answers
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Questions related to Electron Microscopy
We cannot identify this structure, EM of Drosophila larvae near the NMJ. Does anybody have any ideas?
07 July 2018 5,760 1 View
Dear colleagues, Preparation of blocks for FIB-SEM tomography is a little tricky process. Which design do you prefer and which technique and tricks use in the preparation? I make the blocks...
30 June 2018 500 1 View
Dear colleagues, We have prepared a few 6 mm sapphire discs with cells monolayers by HPF, they are in liquid nitrogen now. These disks are too big for conventional Leica freeze substitution...
20 June 2018 8,493 7 View
Recently, I read a paper about scanning ultrafast electron microscopy (SUEM) ( Nano Lett. 2017, 17, 3675−3680). Using this method, the author can directly image the dynamics of photoexcited...
07 June 2018 7,914 1 View
06 June 2018 810 1 View
I have started to work with isolation of exosomes and I used the protocol from this paper (ultracentrifuge): "Isolation and characterization of exosomes from cell culture supernatants and...
06 June 2018 8,964 2 View
I'm new to TEM, I have some difficulties in mitochondrial identification. I am not sure which is mitochondria. Can anyone help me?
06 June 2018 3,886 0 View
06 June 2018 8,093 8 View
The tissue was embbeded in Spurr Resin. The infiltration process was 1:3, 1:2, 1:1, 2:1, Pure (Resin x Acetone). When I went to make the cut, I coudn't cut nothing. Is there any protocol to...
25 May 2018 7,932 2 View
Dear fellow researchers, does anyone have an idea about activity of typical standards for Th (ThO2 - Thorium oxide) and U (UO2 - uranite) used for EPMA/WDS? What are your experience? Is it above...
05 May 2018 551 3 View
Hi guys, I will appreciate if somebody could give me some suggestions on how to perform transmission electron microscopy on structures which have been grown inside a chip like...
05 May 2018 9,769 3 View
05 May 2018 7,570 3 View
what the difference between them in Gold nanoparticles characterization?
05 May 2018 5,298 3 View
Hi, Do you know what is the difference between Uranyl Magnesium Acetate and Uranyl Acetate? I am planing to prepare exosome samples embedded into EPON. And in protocol I found it was used...
05 May 2018 6,562 9 View
I am in the midst of doing a large scale study using serial transmission electron microscopy. It would be helpful if we had a scanning EM here at Texas A&M but we do not have one currently. We...
20 April 2018 2,899 2 View
Hi guys, I have some ampules of formaldehyde, 16%, methanol free, ultra pure (Polysciences cat. number 18814-10) and I would like to use them for transmission electron microscopy with...
04 April 2018 4,540 8 View
04 April 2018 4,230 2 View
Hi all, I have recently seen an Environmental Scanning Electron Microscopy time-lapse video of a plant leaf, showing stomata either openning or closing. All I remember is that the video was...
04 April 2018 6,771 4 View
According to pharmacopoeia 8, section 5.14 and 5.2.3; In viral vector production, some tests must be done as in-process tests after cell harvest for control cells (cells at or beyond the doubling...
04 April 2018 2,613 0 View
While making AgNPs by using Plant extracts and AgNO3 (1mM concentration in 100 ml Distilled water), the quantity of Nanoparticles obtained after centrifugation is very less (less than 1 mg). How...
22 March 2018 5,051 2 View
Currently I want to check the localization of my target protein which is fused to myc tag with electron microscopy. Does anyone have experience using anti myc with high affinity to be used for...
02 February 2018 9,797 7 View
Looking through the literature, there are many slightly different techniques for fixing biological specimens using different pre-post fixatives, mainly paraformaldehyde, gluteraldehyde,...
22 January 2018 3,011 1 View
Hi, Perhaps I've been searching wrong, but I've been unable to find a satisfactory answer for a good extracellular cryoprotectant in high pressure freezing. I know there are many options but each...
08 January 2018 3,548 5 View
01 January 2018 6,440 1 View