Q&A for issues regarding electron microscopy | Contact experts in Electron Microscopy to get answers
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Questions related to Electron Microscopy
I was just wondering...in transmission electron microscopy(TEM) do vesicles (microvesicles or exosomes) have to look like "white dots"(like in much figures in articles) ? or can they have a more...
04 April 2017 5,800 0 View
Hi guys, I was observing some microglial primary cultured cells with the transmission electron microscope and I found some structures I never seen. I really appreciate if someone could provide to...
04 April 2017 3,887 15 View
Biological samples with details of submicrometer scale. Normal ebon embedded specimens ultracut to thin lamellas with thickness of 50-60 nm on 400Mesh Copper grids. I would need longer exposure...
04 April 2017 2,787 11 View
I'm recently concerning ultramicrotomy of SiC/ZrC film for TEM sample preparation. The film lies on metal intermediate layer, which itself lies on alloy substrates, so in such sandwich structure...
04 April 2017 5,415 4 View
Dear all, A lab asked to our electron microscopy facility to analyze whether antidepressants change autophagy in mouse hippocampus neurons with transmission electron microscopy. In order to do...
04 April 2017 2,841 7 View
03 April 2017 1,667 6 View
Hi all, So I will isolate my microvesicles from my cells soon, and will have to fix them for electron microscopy.my only concern here is that I got a protocol of fixation for biopsy pieces.Thus I...
03 March 2017 4,189 7 View
I am trying to convert some cif files to .xyz file format for TEM simulation with QSTEM. All cif files obtained via Findit2009 and I followed the guideline:...
03 March 2017 10,153 11 View
Electron microscopist, Transmission electron microscopy
03 March 2017 4,771 1 View
I´d like to ask, what can be an origin of the dark (electron-dense) objects (whole first picture) and in the second picture (chloroplast), it is present in the very left side, right side and above...
03 March 2017 2,358 6 View
Hi, I'm looking for electrical synapse. For the purpose to documenting its existence, how the approach? Electrophysiological record, Electron microscopy, Immunohistochemistry with connexin, and...
03 March 2017 6,290 1 View
if you have two images of SEM and TEM only (without any data on them) How can you differentiate between two images of SEM and TEM ? Just take a look
03 March 2017 6,292 7 View
For example in which case you prefer to use SIMS rather than Auger electron microscopy?
03 March 2017 7,488 2 View
Hi, I am planning to do EM analysis on exosomes derived from neural progenitor cells. I have quantified the amount of exosomes that I got from my prep by BCA assay and it gave me a concentration...
06 February 2017 7,229 1 View
Hi colleagues, how long I can preserve the specimens in glutaraldehyde for electron microscope examination.
02 February 2017 7,062 17 View
02 February 2017 8,465 1 View
Dear all, Does anybody know a good protocol for electron diffraction using TEM for proteins? I would like to try it. Thank you!
22 January 2017 10,034 5 View
Sub-micrometer particles are usually measured by electron microscopy or by dynamic light scattering. Can a flow cytometer be used instead to distinguish between particle populations below 1...
12 January 2017 2,681 6 View
Immunogold staining is often used for observing biological samples (and exosomes) using TEM. In my knowledge, there are 2 papers (which were published in 90s) which showed that immunogold staining...
01 January 2017 831 2 View
01 January 2017 4,061 6 View
If a part of the energy that an electron carries is transferred to the specimen, several processes might occur leading to the generation of the above signals.
01 January 2017 5,674 0 View
01 January 2017 2,052 7 View
Regarding the Transmission Electron microscopy of Mice organs: spleen, liver , kidney and intestines. Does anyone know what is this?
27 December 2016 2,675 3 View
12 December 2016 418 4 View