Q&A for issues regarding electron microscopy | Contact experts in Electron Microscopy to get answers
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Questions related to Electron Microscopy
For example in which case you prefer to use SIMS rather than Auger electron microscopy?
03 March 2017 7,471 2 View
Hi, I am planning to do EM analysis on exosomes derived from neural progenitor cells. I have quantified the amount of exosomes that I got from my prep by BCA assay and it gave me a concentration...
06 February 2017 7,204 1 View
Hi colleagues, how long I can preserve the specimens in glutaraldehyde for electron microscope examination.
02 February 2017 7,042 17 View
02 February 2017 8,451 1 View
Dear all, Does anybody know a good protocol for electron diffraction using TEM for proteins? I would like to try it. Thank you!
22 January 2017 10,021 5 View
Sub-micrometer particles are usually measured by electron microscopy or by dynamic light scattering. Can a flow cytometer be used instead to distinguish between particle populations below 1...
12 January 2017 2,662 6 View
Immunogold staining is often used for observing biological samples (and exosomes) using TEM. In my knowledge, there are 2 papers (which were published in 90s) which showed that immunogold staining...
01 January 2017 814 2 View
01 January 2017 4,042 6 View
If a part of the energy that an electron carries is transferred to the specimen, several processes might occur leading to the generation of the above signals.
01 January 2017 5,655 0 View
01 January 2017 2,027 7 View
Regarding the Transmission Electron microscopy of Mice organs: spleen, liver , kidney and intestines. Does anyone know what is this?
27 December 2016 2,662 3 View
12 December 2016 400 4 View
can we identify theta prime and theta double prime phase from SAED pattern and how?
12 December 2016 4,409 2 View
07 December 2016 3,281 2 View
I´m wondering how common is the use of the morphometric parameter "Mitochondrial area" in biomedicine applied ultrastructural studies with transmission electron microscopy.
11 November 2016 9,898 0 View
We are facing problems with tissue preservation. We have both mechanical damage in the tissue may be resulting from handling and broken cell membranes indicating unproperly set osmolarity in the...
11 November 2016 2,501 5 View
Dear all, I used carbon coated copper grid 200 mesh for my sample (gold nanoclusters dispersed in ethanol) to get images. Sometimes I got different types of ring-shaped damage after few...
11 November 2016 522 10 View
In percentage of crystal-sample distance and/or working distance
10 October 2016 10,023 1 View
Hi I would want try EM (negative stain method) with my sample but in size exclusion column I identify 3 different oligomers. Can I do EM? or I need a homogeneous sample. Thank you
10 October 2016 6,480 3 View
10 October 2016 1,230 3 View
In SEM, it is written that the Number of secondary electrons can be greater than the number of incoming (primary) electrons. Again, I read in various sources that the Maximum yield (No. of emitted...
27 September 2016 1,465 4 View
Osmium Tetroxide is traditionally used in electron microscopy both as a fixative and a heavy metal stain. But it too expensive, toxic and volatile.
26 September 2016 9,474 4 View
In the wet transfer method (pmma assisted method), after using the TEM grid to fish up the PMMA film with MoS2, we need to dissolve the PMMA film. Following a lot of literature, I use acetone to...
20 September 2016 9,800 6 View
09 September 2016 4,258 5 View