Q&A for issues regarding electron microscopy | Contact experts in Electron Microscopy to get answers
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Questions related to Electron Microscopy
what the difference between them in Gold nanoparticles characterization?
05 May 2018 5,386 3 View
Hi, Do you know what is the difference between Uranyl Magnesium Acetate and Uranyl Acetate? I am planing to prepare exosome samples embedded into EPON. And in protocol I found it was used...
05 May 2018 6,693 9 View
I am in the midst of doing a large scale study using serial transmission electron microscopy. It would be helpful if we had a scanning EM here at Texas A&M but we do not have one currently. We...
20 April 2018 3,020 2 View
Hi guys, I have some ampules of formaldehyde, 16%, methanol free, ultra pure (Polysciences cat. number 18814-10) and I would like to use them for transmission electron microscopy with...
04 April 2018 4,675 8 View
04 April 2018 4,377 2 View
Hi all, I have recently seen an Environmental Scanning Electron Microscopy time-lapse video of a plant leaf, showing stomata either openning or closing. All I remember is that the video was...
04 April 2018 6,890 4 View
According to pharmacopoeia 8, section 5.14 and 5.2.3; In viral vector production, some tests must be done as in-process tests after cell harvest for control cells (cells at or beyond the doubling...
04 April 2018 2,723 0 View
While making AgNPs by using Plant extracts and AgNO3 (1mM concentration in 100 ml Distilled water), the quantity of Nanoparticles obtained after centrifugation is very less (less than 1 mg). How...
22 March 2018 5,163 2 View
Currently I want to check the localization of my target protein which is fused to myc tag with electron microscopy. Does anyone have experience using anti myc with high affinity to be used for...
02 February 2018 9,909 7 View
Looking through the literature, there are many slightly different techniques for fixing biological specimens using different pre-post fixatives, mainly paraformaldehyde, gluteraldehyde,...
22 January 2018 3,134 1 View
Hi, Perhaps I've been searching wrong, but I've been unable to find a satisfactory answer for a good extracellular cryoprotectant in high pressure freezing. I know there are many options but each...
08 January 2018 3,678 5 View
01 January 2018 6,553 1 View
We have a sonication bath, but I was just wondering if purchasing a probe sonicator would lead to better disaggregation of viral particles for both purification and electron microscopy purposes,...
01 January 2018 6,344 1 View
I'm making a model of cortex with an excitatory layer (e.g. layer 2/3 pyramidal neurons) and an inhibitory layer (e.g. gabaergic interneurons). Right now I'm refining the connection matrix. I...
01 January 2018 4,099 4 View
I want to prepare lactic acid bacteria from SEM study
01 January 2018 2,405 3 View
Hello there, I was asked to do a quantitative EDS analysis on a bone sample from an archeological site (bronze age). The sample is a piece of a bone (fistula) that was found fractured. The...
01 January 2018 9,475 8 View
https://www.imagescience.de/download.html Reading this page, I have little idea what many of these terms mean ...Can anyone recommend some links that will enable me to understand how to...
11 December 2017 7,093 1 View
I learned that TEM images gathered by elastic scattering of which diffraction angles pretty small. In my intuition, the image maybe 'top-view' of some atoms in sample and distances between dots in...
11 November 2017 10,037 2 View
Does any one know a good reagent (or protocol) to stain nucleic acids (RNA) for electron microscopy (either negative stain or positive stain)? p.s. This is for single particle analysis. Thanks!
01 November 2017 3,898 3 View
Can anyone please suggest me a way to visualize folic acid (in folic acid decorated nanoparticles) under Transmission Electron Microscopy? Any tagging methods or literature in this regards will be...
22 October 2017 1,862 3 View
Hi everyone, I've got TEM micrographs from colleaque (who is no longer cooperate with our team), but without description. Micrographs present BLM cells (melanoma) treated with DMSO (control...
19 October 2017 8,671 4 View
scanning electron microscopy of nematodes
10 October 2017 7,256 1 View
Hi, I have a hydrogel scaffold seeded with schwann cells to study myelination process. now I want to fix it and see it with transmission electron microscopy. i've read some articles mainly about...
10 October 2017 2,649 8 View
10 October 2017 7,403 5 View