Q&A for issues regarding electron microscopy | Contact experts in Electron Microscopy to get answers
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Questions related to Electron Microscopy
We have a sonication bath, but I was just wondering if purchasing a probe sonicator would lead to better disaggregation of viral particles for both purification and electron microscopy purposes,...
01 January 2018 6,202 1 View
I'm making a model of cortex with an excitatory layer (e.g. layer 2/3 pyramidal neurons) and an inhibitory layer (e.g. gabaergic interneurons). Right now I'm refining the connection matrix. I...
01 January 2018 3,981 4 View
I want to prepare lactic acid bacteria from SEM study
01 January 2018 2,236 3 View
Hello there, I was asked to do a quantitative EDS analysis on a bone sample from an archeological site (bronze age). The sample is a piece of a bone (fistula) that was found fractured. The...
01 January 2018 9,350 8 View
https://www.imagescience.de/download.html Reading this page, I have little idea what many of these terms mean ...Can anyone recommend some links that will enable me to understand how to...
11 December 2017 7,004 1 View
I learned that TEM images gathered by elastic scattering of which diffraction angles pretty small. In my intuition, the image maybe 'top-view' of some atoms in sample and distances between dots in...
11 November 2017 9,927 2 View
Does any one know a good reagent (or protocol) to stain nucleic acids (RNA) for electron microscopy (either negative stain or positive stain)? p.s. This is for single particle analysis. Thanks!
01 November 2017 3,740 3 View
Can anyone please suggest me a way to visualize folic acid (in folic acid decorated nanoparticles) under Transmission Electron Microscopy? Any tagging methods or literature in this regards will be...
22 October 2017 1,757 3 View
Hi everyone, I've got TEM micrographs from colleaque (who is no longer cooperate with our team), but without description. Micrographs present BLM cells (melanoma) treated with DMSO (control...
19 October 2017 8,526 4 View
scanning electron microscopy of nematodes
10 October 2017 7,161 1 View
Hi, I have a hydrogel scaffold seeded with schwann cells to study myelination process. now I want to fix it and see it with transmission electron microscopy. i've read some articles mainly about...
10 October 2017 2,516 8 View
10 October 2017 7,316 5 View
Normally I used to follow SEM imaging investigation tool to find out the exact morphology, but the SEM images are taken once after drying the solution (and that too doesn't give clear indication...
10 October 2017 6,474 5 View
I didn't find any literature on how these copper or other metal grids are made. Do you have any literature or idea about it?
10 October 2017 7,814 4 View
Please share with me Your tips :)
09 September 2017 7,610 4 View
A widely accepeted way to estimate the dislocation density is X-Ray diffraction but which plane do I consider for estimating the disloaction density in the material. Similarly for TEM and EBSD...
08 September 2017 7,427 15 View
Planning on doing some Bluo-Gal staining on tissue and subsequent analysis by electron microscopy. 1 gram of the Bluo-Gal reagent from Gold Biotechnology is ~100$, about 25% of the cost of...
14 August 2017 9,895 1 View
08 August 2017 3,007 1 View
I would like to analyse some mESCs by Scanning Electron Microscope but am unsure of the most appropriate protocol to prep the sample. Would it be okay to - 1. Grow them on glass coverslips 2. Fix...
08 August 2017 3,429 6 View
Hi guys, I would like to do preembedding immunogold on cell cultures. I followed different protocols and I tried to localize different proteins, with both Nanogold (1.4nm) and Aurion (0.8nm) gold...
25 July 2017 5,823 9 View
Many taxonomic complexes still remain biological enigma.in some cases electron microscopy has been helpful to some extent,while chemotaxonmy proved to be of little help.At this juncture came the...
10 July 2017 9,718 9 View
During TEM imaging of martensitic steel by CS corrected omega filter TEM, usually we have magnetic problem which effect on the alignments. Beside the reducing thickness by FIB, is there any other...
10 July 2017 7,649 3 View
07 July 2017 3,844 10 View
In the past I've used TEM to count myelinated and unmyelinated fibres in peripheral nerves (e.g. sciatic nerve), but in my current institution this is looking like it would be very expensive and...
07 July 2017 727 1 View