Q&A for issues regarding electron microscopy | Contact experts in Electron Microscopy to get answers
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Questions related to Electron Microscopy
Many thanks in advance.
16 January 2024 6,173 4 View
This is to know the orientation of the crystals in the peaks (hkl) in the (XRD) pattern.
05 January 2024 2,813 5 View
Ryves D.B. (2006, 2009) proposed the use of formulas for calculating the Diatom Dissolution Index (DDI). However, determining the dominant species of diatoms and classifying them into levels (1-4)...
02 August 2023 1,645 3 View
Hi everyone. I wanted to know how I can numerically calculate the intensity of a surface plasmon mode in photonic crystal fiber.
06 July 2023 6,524 1 View
Dear all, I will really appreciate if someone could give me some suggestions to how to embed (fixation, postfixation, which resin) and in particular how to cut Diatoms algae, in order to...
17 January 2023 8,047 4 View
Recently, I have obtained several SAED patterns in real space units. Can someone help me how to analyze the pattern?
09 December 2022 1,165 0 View
Respected members, I am doing a study on Co, Cr, V and Ni based medium entropy alloys (i.e. NiCoCr, NiCoV and NiCoVCr) which are single phase FCC (confirmed through XRD). While doing the XPS...
08 December 2022 468 0 View
Hi, I am trying to standardize the culture of Zebrafish spinal cord ependymal cells. I dissect the spinal cord. Dissociate the spinal cord by trypsinization and sort the GFP labelled...
31 October 2022 2,297 4 View
I am trying to establish new zebrafish knockouts as part of my project. I currently have F2s with a deletion that looks like it induces a premature stop codon. How can I validate that the...
12 May 2022 9,253 1 View
Need information on UPS for CM12 TEM
11 May 2022 2,588 0 View
Hello all, good day. I am a PhD student doing a course project in optimal control and stumbled upon an issue while trying to implement a load current observer for the UPS inverter system...
23 April 2021 5,505 0 View
Hello, We tried to do CLEM experiment on sapphire disk including cryo fixation (Leica ICE) and then cryo substitution using LR white resin. Once the resin is polymerized, we can't remove the...
14 January 2021 5,643 3 View
The TEM data that I received is in .EMD (electron microscopy dataset) format, how do I extract/visualize the data, which software should I use?
28 October 2020 1,841 1 View
I am usually working with electron microscopy images of macromolecules, and we use reciprocal distance units [1/Angstrom] for units in Fourier Space. A repeating signal every e.g. 10 Angstrom...
28 September 2020 5,732 1 View
Hi Guys, I’m having a terrible time trying to perform immunogold pre-embedding transmission electron microscopy, on monolayer cells to do CLEM. Basically, I follow the protocol suggested in many...
08 September 2020 5,737 2 View
The sensitivity of a quadrupole sensor is very crucial for a Mass Spectrometer that uses a quadrupole array for the detection of analytes. What are the factors that affect the sensitivity of this...
01 July 2020 9,360 0 View
Hello, I am looking for a good gold antibody against mouse IgG1 antibodies for labelling ultrathin cryosections and analysis by transmission electron microscopy. Do you have any...
02 June 2020 8,394 1 View
Hepatitis B transmission through sexual contact has been widely recognized. My question is, can it transmit through intact vaginal/anal mucosa? Or it can only be transmitted through microabrasions...
30 April 2020 2,270 0 View
I'm having difficulties in solving a system of 5 equations Mathlab is taking to mush time to respond and it responds
11 April 2020 7,130 0 View
As soluble, untagged GFP is not membrane bound and doesn't contain targeting or signal sequences, one might expect it to be synthesized by free ribosomes and not bound ribosomes. That would mean...
09 March 2020 1,331 3 View
I am wondering what that ghost thick outline, which is around the carbon wire image which I have attached. I did prepare my sample in a different method (sonication-dispersion in Acetone- and...
12 January 2020 3,385 10 View
01 January 2020 1,233 10 View
Hi all! Is there any reference or suggestion which can help us to set the sampling protocols for the sample collection related to electron microscopy (SEM and TEM). As we are working in...
01 January 2020 896 2 View
How can we identify the precipitate /matrix relationship (coherent/incoherent) from coffee bean contrast observed in the precipitates.
02 December 2019 3,591 1 View