Q&A for issues regarding electron microscopy | Contact experts in Electron Microscopy to get answers
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Questions related to Electron Microscopy
Hi everyone. I wanted to know how I can numerically calculate the intensity of a surface plasmon mode in photonic crystal fiber.
06 July 2023 6,353 1 View
Dear all, I will really appreciate if someone could give me some suggestions to how to embed (fixation, postfixation, which resin) and in particular how to cut Diatoms algae, in order to...
17 January 2023 7,825 4 View
Recently, I have obtained several SAED patterns in real space units. Can someone help me how to analyze the pattern?
09 December 2022 995 0 View
Respected members, I am doing a study on Co, Cr, V and Ni based medium entropy alloys (i.e. NiCoCr, NiCoV and NiCoVCr) which are single phase FCC (confirmed through XRD). While doing the XPS...
08 December 2022 287 0 View
Hi, I am trying to standardize the culture of Zebrafish spinal cord ependymal cells. I dissect the spinal cord. Dissociate the spinal cord by trypsinization and sort the GFP labelled...
31 October 2022 2,122 4 View
Need information on UPS for CM12 TEM
11 May 2022 2,428 0 View
Hello, We tried to do CLEM experiment on sapphire disk including cryo fixation (Leica ICE) and then cryo substitution using LR white resin. Once the resin is polymerized, we can't remove the...
14 January 2021 5,400 3 View
The TEM data that I received is in .EMD (electron microscopy dataset) format, how do I extract/visualize the data, which software should I use?
28 October 2020 1,631 1 View
I am usually working with electron microscopy images of macromolecules, and we use reciprocal distance units [1/Angstrom] for units in Fourier Space. A repeating signal every e.g. 10 Angstrom...
28 September 2020 5,261 1 View
Hi Guys, I’m having a terrible time trying to perform immunogold pre-embedding transmission electron microscopy, on monolayer cells to do CLEM. Basically, I follow the protocol suggested in many...
08 September 2020 5,499 2 View
Hello, I am looking for a good gold antibody against mouse IgG1 antibodies for labelling ultrathin cryosections and analysis by transmission electron microscopy. Do you have any...
02 June 2020 8,207 1 View
As soluble, untagged GFP is not membrane bound and doesn't contain targeting or signal sequences, one might expect it to be synthesized by free ribosomes and not bound ribosomes. That would mean...
09 March 2020 1,125 3 View
I am wondering what that ghost thick outline, which is around the carbon wire image which I have attached. I did prepare my sample in a different method (sonication-dispersion in Acetone- and...
12 January 2020 3,194 10 View
01 January 2020 1,036 10 View
Hi all! Is there any reference or suggestion which can help us to set the sampling protocols for the sample collection related to electron microscopy (SEM and TEM). As we are working in...
01 January 2020 720 2 View
How can we identify the precipitate /matrix relationship (coherent/incoherent) from coffee bean contrast observed in the precipitates.
02 December 2019 3,401 1 View
Hi all, I have synthesized a catalyst for oxygen reduction reaction (ORR). Polyvinylpyrrolidinon was used as as source of C,N while it was pyrolyzed with FeSO4 as a source of Fe and S at 800C in...
09 September 2019 7,404 5 View
I am looking for a contrast agent specific of plant cell-wall's lignin suited for Scaning Electron Microscopy or X-Ray computed tomography. Many thanks !
09 September 2019 4,046 2 View
Good day to everyone! The situation - I have a plunge-frozen sample on TEM grid and I need to perform cryo-ultramicrotomy of the sample together with the grid (that is I need to cut perpendicular...
09 September 2019 3,824 2 View
i am trying to purify bacteriohage phix174, in gradient i got one separate band. but when i see that sample in negative stain, i got some kind of contamination in sample either bacterial membrane...
22 June 2019 7,676 5 View
06 June 2019 1,484 4 View
Hey! Thanks!! Phil
06 June 2019 8,728 1 View
Hello, I want to see if my E. coli is hyper flagellated by Electron microscopy without destructing the flagella on the surface of the strain. Is there any protocol to help me? I would like to know...
29 May 2019 9,626 4 View
Hello everyone, I am going to do some analysis of fixed tissue with electron microscopy. Before that, I should prepare my sample that attached on glass surface. Does anybody know how to do...
05 May 2019 4,880 1 View