I did immunoelectronmicroscopy silverstaing on cryosections of bovine retina tissue. I found a dark black background after staining on the slides along with the tissue. Is it a common phenomenon as I do not observe it in my positive control?
You can refer to the following links to get certain valuable details:-
1. http://cdn.intechopen.com/pdfs/30339.pdf
2. http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0105359
Dear Shweta Suiwal, to reply to your question properly you should tell more precisely about the technique (test & control samples) "silverstaining" you used for your IEM-study and perhaps also about your observation target (determining cells - nerves?, localization of metals=AMG?, other purpose?).
Regards, Wolfgang
Dear Wolfgang H. Muss.
I performed immunolabelling with my self generated antibodies on 20micrometer thick cryosection of retina.
As a positive control I used known antibody and only secondary antibody as negative control. After completing all the immunolabelling steps I did silverstaining of my section. After staining my positive control tissue look light brown stained. But in case of my test antibodies my tissue section also look light brown stained but along with it I also see dark black background surrounding my tissue on slide. So is it common to have this background on slides?
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