Why the VFA values in negative?

I wish to know about the reason behind getting VFA values in negative. Initially for few days, I got positive values (91mg/L to 13.61 mg/L) and for the past two days my values went to -20.09 and...

09 October 2018 5,731 2 View

Is there any report about the following strains isolated from insect gut?

I have found out following strains from insect gut flora Devosia chinhatensis, Pseudomonas stutzeri, Serratia nematodiphila through 16sRNA analysis. There are many reports about these strains at...

02 March 2015 5,740 4 View

Is there any report on the gut flora of H.armigera or all Lepidoptera with the following strains?

01 February 2015 5,698 3 View

Where can I find dichotomous keys for the genus Fulmekiola?

11 December 2014 9,807 3 View

Where can I get the reference sequence for 16S rDNA?

I have analyzed gut flora of Helicoverpa and found B. pumilius, with some strain identification by 16S rDNA analysis. I want to check these strains via nucleotide diversity by DNA sp software. I...

11 December 2014 9,681 8 View

Can someone tell me which is good vector for metagenomics study?

I am looking for a good vector for the cloning of gut-micflora metagenome. The source of metagenome is insect gut. I isolated a number of cultures and did 16sRna sequencing. On the other side we...

31 December 2013 5,628 6 View

Can someone help me to analyze a 16sRNA sequence?

Can someone assist me regarding the analysis of 16sRNA sequences. After my PCR, I sent my number of samples for sequencing. I got report from them as Forward Trimmed sequence and Reverse trimmed...

11 December 2013 9,086 4 View

Can someone help with a restriction enzyme digestion problem?

I digested pcr products by MboI enzyme, expected 2 bands one at 296 and another at 72bp. I got respectively in few samples which means they are resistant, the wild type will not show any cut...

10 November 2013 984 6 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Shall I skip the measurement model in SmartPLS?

Hello everyone, I had run a mediation model in SmartPLS using questionnaires that were already validated among my statistical population. A professor who edited my paper -though she is not...

01 March 2021 3,052 3 View

SEM or Multiple Regression for an analysis without Latent Variables?

Hello, I have three hypothesis to test, all with the same IV. I am testing if the effect of the IV on the first DV is mediated by awe. I am then measuring whether the effect of the IV on a...

26 February 2021 8,399 5 View

Can anyone help with Schistosoma identification?

Can anyone identify what kind of Schistosome species this might be? Morphological features of cercariae and adults resemble Trichobilharzia, though I am fairly new to the field of parasitology...

25 February 2021 7,760 6 View

IF troubleshooting: looking at an intracellular protein that localizes to the cell's and how to reduce increase in signal due to cell ruffles?

I am currently trying to stain for PI3KBeta in MDA-MB-231 cells. My current issue is that 1) beta stains all over the cell and 2) there is an increase in signal at the edge of the cell and this...

24 February 2021 8,582 1 View

How to clean up TEM grid with plasma cleaner?

I need to clean the hydrocarbon contamination on the carbon substrate of TEM grid. However, I heard that substrate could be broken by the powerful plasma. My substrate is pure carbon, with ~3nm,...

23 February 2021 3,262 5 View

How we can measure Kemp % in wool without using of benzene?

Would you please let me to know about the methods for measurement of medullation and Kemp % on animal? My problem is that the wool sampling and sending to laboratory is not possible. So I would...

21 February 2021 7,260 4 View

Magnetismic Gravity in the universe?

MAGNETISMIC GRAVITY What is magnetism? Something magnetic has a + and - charge. If 2 + or - are pushed if + and - are pulled. But what is the main reason for this? Why does magnetism have a pull...

21 February 2021 9,538 3 View