Q&A for issues regarding electron microscopy | Contact experts in Electron Microscopy to get answers
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Questions related to Electron Microscopy
Normally I used to follow SEM imaging investigation tool to find out the exact morphology, but the SEM images are taken once after drying the solution (and that too doesn't give clear indication...
10 October 2017 6,571 5 View
I didn't find any literature on how these copper or other metal grids are made. Do you have any literature or idea about it?
10 October 2017 7,920 4 View
Please share with me Your tips :)
09 September 2017 7,683 4 View
A widely accepeted way to estimate the dislocation density is X-Ray diffraction but which plane do I consider for estimating the disloaction density in the material. Similarly for TEM and EBSD...
08 September 2017 7,519 15 View
The batteries on our Toshiba USP 1600ep (for our LTQ-XL mass spectrometer) have gone bad. Is there an alternative to this very expensive UPS? How difficult is it to replace the batteries ourselves?
02 September 2017 4,487 1 View
Planning on doing some Bluo-Gal staining on tissue and subsequent analysis by electron microscopy. 1 gram of the Bluo-Gal reagent from Gold Biotechnology is ~100$, about 25% of the cost of...
14 August 2017 10,000 1 View
08 August 2017 3,107 1 View
I would like to analyse some mESCs by Scanning Electron Microscope but am unsure of the most appropriate protocol to prep the sample. Would it be okay to - 1. Grow them on glass coverslips 2. Fix...
08 August 2017 3,507 6 View
Hi guys, I would like to do preembedding immunogold on cell cultures. I followed different protocols and I tried to localize different proteins, with both Nanogold (1.4nm) and Aurion (0.8nm) gold...
25 July 2017 5,918 9 View
Many taxonomic complexes still remain biological enigma.in some cases electron microscopy has been helpful to some extent,while chemotaxonmy proved to be of little help.At this juncture came the...
10 July 2017 9,832 9 View
During TEM imaging of martensitic steel by CS corrected omega filter TEM, usually we have magnetic problem which effect on the alignments. Beside the reducing thickness by FIB, is there any other...
10 July 2017 7,737 3 View
07 July 2017 3,938 10 View
In the past I've used TEM to count myelinated and unmyelinated fibres in peripheral nerves (e.g. sciatic nerve), but in my current institution this is looking like it would be very expensive and...
07 July 2017 825 1 View
07 July 2017 7,972 3 View
07 July 2017 8,146 10 View
Do anyone know if there is a Electron microscopy facility offering SEM for Exosome ? We have exosome isolated from serum and want to visualize it through SEM. Thanks Deep
06 June 2017 7,625 2 View
Dear Ladies and Gentlemen, does one of you know what exactly the pixel with is in electron microscopy? I found definitions on the web, but nobody gave any serious sources. So far; pixel with...
06 June 2017 2,382 6 View
From my understanding, I need to focus the beam on sample by reduce the intensity. Then, click diffraction mode, and then increases intensity knob. For some reason, I still don't see any kikuchi...
06 June 2017 5,936 3 View
Hi All, I am planning to perform TEM analysis of Al-Zn alloys. Just wondering if someone have recipe for TEM samples preparation for such soft alloys? Have a great weekend Cheers Abdul
06 June 2017 806 8 View
03 June 2017 1,126 5 View
03 June 2017 3,435 7 View
I need to use quantitative electron tomography technique to study and model carbon nano tubes into polymer matrix. I am completely a new user for electron microscopy technique. I came to know...
05 May 2017 352 2 View
I need to know how can i integrate different images obtained from transmission electron microscopy(TEM). The algorithm adds images obtained through different angles and helps to construct a 3D...
05 May 2017 4,493 2 View
We want to conduct immunogold labelling for scanning electron microscopy. As this is our first experience, I would like to know how long the samples can be kept in the fixation solution after...
05 May 2017 6,376 3 View