Q&A for issues regarding electron microscopy | Contact experts in Electron Microscopy to get answers
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Questions related to Electron Microscopy
During TEM imaging of martensitic steel by CS corrected omega filter TEM, usually we have magnetic problem which effect on the alignments. Beside the reducing thickness by FIB, is there any other...
07 July 2017 7,886 3 View
Many taxonomic complexes still remain biological enigma.in some cases electron microscopy has been helpful to some extent,while chemotaxonmy proved to be of little help.At this juncture came the...
07 July 2017 8,028 10 View
Do anyone know if there is a Electron microscopy facility offering SEM for Exosome ? We have exosome isolated from serum and want to visualize it through SEM. Thanks Deep
06 June 2017 7,528 2 View
Dear Ladies and Gentlemen, does one of you know what exactly the pixel with is in electron microscopy? I found definitions on the web, but nobody gave any serious sources. So far; pixel with...
06 June 2017 2,283 6 View
From my understanding, I need to focus the beam on sample by reduce the intensity. Then, click diffraction mode, and then increases intensity knob. For some reason, I still don't see any kikuchi...
06 June 2017 5,857 3 View
Hi All, I am planning to perform TEM analysis of Al-Zn alloys. Just wondering if someone have recipe for TEM samples preparation for such soft alloys? Have a great weekend Cheers Abdul
06 June 2017 669 8 View
03 June 2017 1,046 5 View
03 June 2017 3,344 7 View
I need to use quantitative electron tomography technique to study and model carbon nano tubes into polymer matrix. I am completely a new user for electron microscopy technique. I came to know...
05 May 2017 246 2 View
I need to know how can i integrate different images obtained from transmission electron microscopy(TEM). The algorithm adds images obtained through different angles and helps to construct a 3D...
05 May 2017 4,376 2 View
We want to conduct immunogold labelling for scanning electron microscopy. As this is our first experience, I would like to know how long the samples can be kept in the fixation solution after...
05 May 2017 6,304 3 View
I'm recently concerning ultramicrotomy of SiC/ZrC film for TEM sample preparation. The film lies on metal intermediate layer, which itself lies on alloy substrates, so in such sandwich structure...
05 April 2017 8,179 2 View
I was just wondering...in transmission electron microscopy(TEM) do vesicles (microvesicles or exosomes) have to look like "white dots"(like in much figures in articles) ? or can they have a more...
04 April 2017 5,788 0 View
Hi guys, I was observing some microglial primary cultured cells with the transmission electron microscope and I found some structures I never seen. I really appreciate if someone could provide to...
04 April 2017 3,856 15 View
Biological samples with details of submicrometer scale. Normal ebon embedded specimens ultracut to thin lamellas with thickness of 50-60 nm on 400Mesh Copper grids. I would need longer exposure...
04 April 2017 2,769 11 View
04 April 2017 5,399 4 View
Dear all, A lab asked to our electron microscopy facility to analyze whether antidepressants change autophagy in mouse hippocampus neurons with transmission electron microscopy. In order to do...
04 April 2017 2,784 7 View
03 April 2017 1,650 6 View
Hi all, So I will isolate my microvesicles from my cells soon, and will have to fix them for electron microscopy.my only concern here is that I got a protocol of fixation for biopsy pieces.Thus I...
03 March 2017 4,172 7 View
I am trying to convert some cif files to .xyz file format for TEM simulation with QSTEM. All cif files obtained via Findit2009 and I followed the guideline:...
03 March 2017 10,128 11 View
Electron microscopist, Transmission electron microscopy
03 March 2017 4,755 1 View
I´d like to ask, what can be an origin of the dark (electron-dense) objects (whole first picture) and in the second picture (chloroplast), it is present in the very left side, right side and above...
03 March 2017 2,341 6 View
Hi, I'm looking for electrical synapse. For the purpose to documenting its existence, how the approach? Electrophysiological record, Electron microscopy, Immunohistochemistry with connexin, and...
03 March 2017 6,276 1 View
if you have two images of SEM and TEM only (without any data on them) How can you differentiate between two images of SEM and TEM ? Just take a look
03 March 2017 6,269 7 View