Q&A for issues regarding electron microscopy | Contact experts in Electron Microscopy to get answers
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Questions related to Electron Microscopy
after some time battery die without any reason.I want to know how did the batteries fail to charge.They are made so that it help in the backup of the system.When u do not have power supply.
15 November 2019 8,072 0 View
Dear All I am preparing mutant HBV from HepG2 cells by transfecting the recombinant plasmid DNA. Usually, I concentrate HBV by PEG precipitation. But high concentration of PEG affects HBV...
20 October 2019 9,267 0 View
Hi all, I have synthesized a catalyst for oxygen reduction reaction (ORR). Polyvinylpyrrolidinon was used as as source of C,N while it was pyrolyzed with FeSO4 as a source of Fe and S at 800C in...
09 September 2019 7,492 5 View
I am looking for a contrast agent specific of plant cell-wall's lignin suited for Scaning Electron Microscopy or X-Ray computed tomography. Many thanks !
09 September 2019 4,143 2 View
Good day to everyone! The situation - I have a plunge-frozen sample on TEM grid and I need to perform cryo-ultramicrotomy of the sample together with the grid (that is I need to cut perpendicular...
09 September 2019 3,930 2 View
I would like to observe the biodistribution of nanoparticles inside cells by TEM. I found in some literature that people are using different types of resins for embedding. In one literature I...
17 July 2019 3,599 2 View
i am trying to purify bacteriohage phix174, in gradient i got one separate band. but when i see that sample in negative stain, i got some kind of contamination in sample either bacterial membrane...
22 June 2019 7,749 5 View
06 June 2019 1,572 4 View
Hey! Thanks!! Phil
06 June 2019 8,799 1 View
Hello, I want to see if my E. coli is hyper flagellated by Electron microscopy without destructing the flagella on the surface of the strain. Is there any protocol to help me? I would like to know...
29 May 2019 9,718 4 View
Hello everyone, I am going to do some analysis of fixed tissue with electron microscopy. Before that, I should prepare my sample that attached on glass surface. Does anybody know how to do...
05 May 2019 4,989 1 View
05 May 2019 9,921 5 View
I am new to electron holography and I am trying to understand the technique. It will be useful if anyone can point me to resources that explain why twin images are observed in electron holography....
05 May 2019 851 2 View
I have many images from electron microscopy of round structures. I am looking for a way to measure the area of these in an automated way. Any suggestions would be very much appreciated.
04 April 2019 9,318 3 View
I am planning to perform analysis of liposomes using TEM. Therefore, i thought of using Osmium tetroxide solution as a staining agent.
21 March 2019 6,351 4 View
Dear all, have you experienced dark lines that are localized primarily within the cells (detailed TEM pics.)? Cells were embedded in epon for TEM (but I experienced the same lines in araldite and...
19 March 2019 4,153 4 View
I have a polymer material and I want to find out what type it is. I want to know its chemical formula. How many Carbons, Hydrogen and Oxygen are there? I already know that Electron Microscopy...
08 March 2019 788 7 View
We would like to do an electron microscopy analysis of mitochondrial morphology in human blood cells. I am planning to use buffy coat for this. Is it possible to do this with a limited amount of...
08 March 2019 4,851 4 View
03 March 2019 7,052 5 View
We have recently purchased 3% glyoxal fixative from EMS and it comes as a 100mL kit that has two reagents that need to be combined and pH'd before use. We do not need all 100mL at once and will...
02 February 2019 8,345 5 View
We are currently measuring mitochondrial length, width, area, type (vesicular or non-vesicular) within electron microscopy images. Since this is performed by manually drawing around each...
02 February 2019 7,707 2 View
The datasheet says: "After polymerization, the block sometimes turns to a light pink or pink/red color. This seems to be quite normal and should not be a cause for concern. " Indeed, after...
23 January 2019 9,158 5 View
01 January 2019 5,755 6 View
I need to prepare formvar-coated grids for TEM. I usually use an uncommon method of a drop of formvar on water, however the formvar film is usually uneven. Another and more common method is by...
12 December 2018 1,079 6 View