Q&A for issues regarding electron microscopy | Contact experts in Electron Microscopy to get answers
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Questions related to Electron Microscopy
Hello, I want to see if my E. coli is hyper flagellated by Electron microscopy without destructing the flagella on the surface of the strain. Is there any protocol to help me? I would like to know...
05 May 2019 9,840 5 View
I am new to electron holography and I am trying to understand the technique. It will be useful if anyone can point me to resources that explain why twin images are observed in electron holography....
05 May 2019 769 2 View
I have many images from electron microscopy of round structures. I am looking for a way to measure the area of these in an automated way. Any suggestions would be very much appreciated.
04 April 2019 9,227 3 View
I am planning to perform analysis of liposomes using TEM. Therefore, i thought of using Osmium tetroxide solution as a staining agent.
21 March 2019 6,259 4 View
Dear all, have you experienced dark lines that are localized primarily within the cells (detailed TEM pics.)? Cells were embedded in epon for TEM (but I experienced the same lines in araldite and...
19 March 2019 4,081 4 View
I have a polymer material and I want to find out what type it is. I want to know its chemical formula. How many Carbons, Hydrogen and Oxygen are there? I already know that Electron Microscopy...
08 March 2019 679 7 View
We would like to do an electron microscopy analysis of mitochondrial morphology in human blood cells. I am planning to use buffy coat for this. Is it possible to do this with a limited amount of...
08 March 2019 4,772 4 View
03 March 2019 6,974 5 View
We have recently purchased 3% glyoxal fixative from EMS and it comes as a 100mL kit that has two reagents that need to be combined and pH'd before use. We do not need all 100mL at once and will...
02 February 2019 8,263 5 View
We are currently measuring mitochondrial length, width, area, type (vesicular or non-vesicular) within electron microscopy images. Since this is performed by manually drawing around each...
02 February 2019 7,623 2 View
The datasheet says: "After polymerization, the block sometimes turns to a light pink or pink/red color. This seems to be quite normal and should not be a cause for concern. " Indeed, after...
23 January 2019 9,073 5 View
01 January 2019 5,653 6 View
I need to prepare formvar-coated grids for TEM. I usually use an uncommon method of a drop of formvar on water, however the formvar film is usually uneven. Another and more common method is by...
12 December 2018 960 6 View
04 December 2018 2,340 5 View
The infectious viruses such as Hepatitis B, C and HIV are endemic in some developing countries. A number of people practice "shaolin" haircut [clean hair shave]. a number of times people...
03 December 2018 8,524 5 View
Hi everybody. I wonder if there is someone here that has successfully worked with TEM grids sold by Electron Microscopy Sciences for the analysis of extracellular vesicles (microvesicles and...
14 November 2018 10,092 1 View
11 November 2018 9,625 1 View
Hello, congratulations for this great project! I'm curious about the portability of this tactile display, specifically the power supply/consumption. I've read it uses Li-ion batteries and it...
05 November 2018 8,596 1 View
The experiment is very simple. It consists of two parallel spark gaps, one spark gap is approximately 0,5 mm wide and the second gap is a little bit wider, about 0,7 mm wide. The parallel distance...
19 October 2018 2,572 4 View
For sample preparation for electron microscopy do I need to prewash with saline and then with the fixative? Or do I need to to leave out the prewash ?
10 October 2018 7,789 0 View
Hey everyone, We have been trying to image some amyloid fibrils under STEM using 2% UAR uranyl acetate replacement stain but we haven't been consistently getting good images. Most of our grids...
10 October 2018 5,024 5 View
Dear colleagues, Did you use HPF for some super-soft tissue like testis? Operation with "sampled" specimens like pieces of tissue, small worms, etc. is pretty clear. But when we have some...
10 October 2018 7,575 15 View
Hi all, I made an error in my recipe for making a PHEM buffer solution and realised that I put 10 x more HEPES into the PHEM buffer than I should have. The working concentration of HEPES in 1 x...
31 July 2018 2,463 8 View
which one is excellent result oriented protocol for fixation of Zebrafish , Danio rerio tissues, for ultramicrotomy on Transmission electron microscopy?
07 July 2018 4,661 0 View