Q&A for issues regarding electron microscopy | Contact experts in Electron Microscopy to get answers
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Questions related to Electron Microscopy
Dear All I am preparing mutant HBV from HepG2 cells by transfecting the recombinant plasmid DNA. Usually, I concentrate HBV by PEG precipitation. But high concentration of PEG affects HBV...
20 October 2019 9,183 0 View
Hi all, I have synthesized a catalyst for oxygen reduction reaction (ORR). Polyvinylpyrrolidinon was used as as source of C,N while it was pyrolyzed with FeSO4 as a source of Fe and S at 800C in...
09 September 2019 7,423 5 View
I am looking for a contrast agent specific of plant cell-wall's lignin suited for Scaning Electron Microscopy or X-Ray computed tomography. Many thanks !
09 September 2019 4,067 2 View
Good day to everyone! The situation - I have a plunge-frozen sample on TEM grid and I need to perform cryo-ultramicrotomy of the sample together with the grid (that is I need to cut perpendicular...
09 September 2019 3,853 2 View
I would like to observe the biodistribution of nanoparticles inside cells by TEM. I found in some literature that people are using different types of resins for embedding. In one literature I...
17 July 2019 3,542 2 View
i am trying to purify bacteriohage phix174, in gradient i got one separate band. but when i see that sample in negative stain, i got some kind of contamination in sample either bacterial membrane...
22 June 2019 7,690 5 View
06 June 2019 1,500 4 View
Hey! Thanks!! Phil
06 June 2019 8,739 1 View
Hello, I want to see if my E. coli is hyper flagellated by Electron microscopy without destructing the flagella on the surface of the strain. Is there any protocol to help me? I would like to know...
29 May 2019 9,639 4 View
Hello everyone, I am going to do some analysis of fixed tissue with electron microscopy. Before that, I should prepare my sample that attached on glass surface. Does anybody know how to do...
05 May 2019 4,903 1 View
05 May 2019 9,855 5 View
I am new to electron holography and I am trying to understand the technique. It will be useful if anyone can point me to resources that explain why twin images are observed in electron holography....
05 May 2019 781 2 View
I have many images from electron microscopy of round structures. I am looking for a way to measure the area of these in an automated way. Any suggestions would be very much appreciated.
04 April 2019 9,245 3 View
I am planning to perform analysis of liposomes using TEM. Therefore, i thought of using Osmium tetroxide solution as a staining agent.
21 March 2019 6,283 4 View
Dear all, have you experienced dark lines that are localized primarily within the cells (detailed TEM pics.)? Cells were embedded in epon for TEM (but I experienced the same lines in araldite and...
19 March 2019 4,091 4 View
I have a polymer material and I want to find out what type it is. I want to know its chemical formula. How many Carbons, Hydrogen and Oxygen are there? I already know that Electron Microscopy...
08 March 2019 701 7 View
We would like to do an electron microscopy analysis of mitochondrial morphology in human blood cells. I am planning to use buffy coat for this. Is it possible to do this with a limited amount of...
08 March 2019 4,785 4 View
03 March 2019 6,989 5 View
We have recently purchased 3% glyoxal fixative from EMS and it comes as a 100mL kit that has two reagents that need to be combined and pH'd before use. We do not need all 100mL at once and will...
02 February 2019 8,281 5 View
We are currently measuring mitochondrial length, width, area, type (vesicular or non-vesicular) within electron microscopy images. Since this is performed by manually drawing around each...
02 February 2019 7,643 2 View
The datasheet says: "After polymerization, the block sometimes turns to a light pink or pink/red color. This seems to be quite normal and should not be a cause for concern. " Indeed, after...
23 January 2019 9,092 5 View
01 January 2019 5,676 6 View
I need to prepare formvar-coated grids for TEM. I usually use an uncommon method of a drop of formvar on water, however the formvar film is usually uneven. Another and more common method is by...
12 December 2018 982 6 View
04 December 2018 2,367 5 View