A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a... | Contact experts in DNA to get answers
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Questions related to DNA
I cloned my designed gene fragment in L4440 vector under the control of two T7-promoters to get a double stradouble-strandednded RNA (dsRNA). This cloned vector is transformed into an HT115...
25 November 2019 7,066 1 View
We made adoptive transference from macrophages of C57BL/6 to Balb/c mice. Now we need to know if those cell migrated to local lesion in the paws and we thought to make a PCR with the DNA extracted...
24 November 2019 7,586 0 View
I am currently working on u937 human monocytes and have tried staining them with cd68 but I have not had any immunofluorescence signal. could anyone give any guidance please? is there another...
22 November 2019 9,541 2 View
I've transformed high-copy plasmid (pGEMT Easy - 13 Kbp) in E.coli (JM109) and did plasmid extraction with commercial kit from Macherey Nagel GigaPrep EF. However, the resulted concentration of...
11 November 2019 3,090 8 View
Hi everybody, I am currently working on my Bachelor Thesis, for which I am performing EMSA on CRISPR/Cas9. I am trying to bind Cas to a specific target, but every time I try I get these multiple...
11 November 2019 3,577 3 View
Hi everyone, I am performing ChIP-seq on a transcription co-factor, for that I had to first crosslink protein-protein with EGS (1.5 mM) for 25 min and then I added 1% formaldehyde for 10 min,...
11 November 2019 7,586 2 View
Hi Everybody, I am aiming to specifically knock down transcript variants of my GOI. Can anyone recommend vendors that design transcript variant specific siRNA? Please only reply if you have...
11 October 2019 9,540 0 View
I am completing my Master's in Biology on small mammal research (Sorex shrews) in Washington State. I am looking for a reliable lab in the US to send tail clips/liver samples to, to sequence the...
10 October 2019 295 2 View
The HGP project utilized white blood cells from female and sperm cells from male candidates to map the human DNA. Since these cells are differentiated, the ratio of heterochromatin to euchromatin...
10 October 2019 5,559 1 View
I'm using a stable sole of TiO2 in a acidic environment and I've tried to dilute it by ethanol and deionized water. but every time I see pin holes. what can I do? thanks
17 September 2019 7,248 6 View
I'm trialling a recently described salting out protocol to extract HMW DNA from insect tissue: https://www.biorxiv.org/node/716332.external-links.html. The yield so far is good (~2-6 ug DNA / 30...
09 September 2019 4,457 6 View
To begin my words, I will show my assumption about the oligonucleotide process. 1. Drying process itself doesn't influence to oligonucleotide quantity. 2. The OD value should be same between first...
09 September 2019 8,062 4 View
I want to collect saliva samples in the Zymo DNA RNA shield in order to extract human DNA and RNA from the saliva. Has anyone tried this, either DNA, RNA or preferably both, and was the quality...
09 September 2019 7,864 1 View
I need some help with this issue. Anyone have arranged protocol? I understand that the first step is the separation of plasma from WBC+RBC and after extraction DNA and qPCR, but it's still unclear...
09 September 2019 1,011 6 View
Hello, I want to simulate multiple interactions between DNA in solution(Ion, pH, Temperature) and plasmonic field. (Ion, pH, Temperature => DNA dimension(Rg, persistence length) and...
09 September 2019 2,600 1 View
I'm running my PCR product on 1% gel (to excise for gel purification, samples to be sequenced). My target amplicon is 1090bp, and I can reliably get a strong, clean band with few non-specific...
09 September 2019 3,840 9 View
Good day, currently I am practicing extracting total DNA using drosophila melanogaster (fruit fly) adult as well as larvae as my sample with Qiagen DNeasy Blood and Tissue Kit. However, I keep...
29 August 2019 666 3 View
I am going to observe the dynamic change of TAM in B16 melanoma, my current gating strategy is CD11bintF4/80hi, but the cell cluster is not very clearly. Is this strategy accurate enough? How to...
26 August 2019 9,390 4 View
08 August 2019 5,152 3 View
Hi! Im trying to calculate ion strength and pH of solution. Components of the solution: 47 ul of 1000 pmol DNA in Elution Buffer (10 mM Tris-HCl pH 8.5) was bound to 3 ul of 10 µM YoYo1...
08 August 2019 3,508 8 View
I tried making stock solution of 100 mg/ml of Ampicillin from anhydrous Ampicillin from Alfa Aesar. I mixed Nuclease free water to 5 g of anhydrous Amp to make it 50 ml. However, it wasn't...
08 August 2019 1,729 7 View
I am currently planning an experiment making an antibody-siRNA conjugate using this kit: TriLink Antibody-Oligonucleotide All-in-One Conjugation Kit,...
30 July 2019 3,161 3 View
Does a paired-samples mean one to one matching of the data between pre-and post tests or is it just the pre- and post- data from the same group taken for the comparison of the mean difference?
29 July 2019 6,538 7 View
For example if the lesion shows hypo or hyper or normal signal intensity in different MRI sequences in all the previously reported studies. How do i compare such a data for a meaningful comparison.
13 July 2019 724 2 View