A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a... | Contact experts in DNA to get answers
2,286 views 3,974 posts
Questions related to DNA
I am looking for some method for phage inactivation with out damage of bacteria, for sterility test of phage preparation by USP 71. Thanks in advance
19 October 2021 5,569 5 View
Hi All, I am trying to get some colonies after the bp reaction but I have been trying this for a while and at this point I have no colonies. I am using the pDONR 221, the fragment is 2.3 kb and...
15 October 2021 1,622 1 View
There are different opinion: I would like to quote from the following link one: https://www.aliem.com/tips-for-interpreting-the-csf-opening-pressure/ "The normal range for CSF is reported...
14 October 2021 9,350 5 View
CD11b+F4/80-Ly6G-CD115+should be monocytes. But what about the CD115- population? Granulocyte-Monocyte Progenitors and Monocyte-Dendritic Cell... In this paper, Fig1. Both GMP and MDP can...
12 October 2021 9,132 0 View
Hi everyone. I am trying to replicate an homology modeling protocol. Right now, I am in the TM refinement part which is made in GROMACS, but I don’t understand how to perform the following...
07 October 2021 4,792 2 View
I have troubles quantifying my tau aggregates"purified" by sequential protein extraction from HEK293 cells, especially with the sarkosyl-insoluble fraction. The pellet was resuspended in 1x PBS,...
07 October 2021 8,335 2 View
I would like to do single cell sequencing of DNA and total RNA in the same cell . My interests on noncoding RNAs. Do you know any cmany doing that? In the publications I read about SIDR-seq. But I...
02 October 2021 4,486 1 View
Should I plan for FFPE sections or direct biopsy tissues? what is the minimum amount/diameter of tissue required for the same?
30 September 2021 8,238 0 View
Hello, I was looking forward to analyzing some samples using the Luciferase assay. However, I wanted to know how much time it would take for the sample to show results once the Luciferin enzyme is...
29 September 2021 1,449 3 View
Hi, We all know that the catalysts provide an alternative (lower energy) pathway for a reaction to occur. For example, the gas reforming reactions (say steam reforming of methane) are highly...
23 September 2021 9,901 5 View
Dear all, I want to prepare a 0.05 M, pH 7 phosphate buffer solution that contain 1% w/v casein and 0.006 M EDTA for proteolytic activity determination. As I know, EDTA and casein only dissolve in...
18 September 2021 6,760 2 View
How to prepare a solution saturated with H2S? I am looking to prepare a NACE Solution ‘A’ which is saturated with H2S in order to do sulfide stress cracking tests. I am planning to put the...
06 September 2021 4,477 12 View
I isolated mRNA from HEK293T cells using oligo dT cellulose, followed by RNA Cleanup and Concentrate kit that includes a DNAse digest. The final mRNA yield was about 6.7ug, with 260/280 and...
26 August 2021 1,682 5 View
shallow or deep foundation subjected to seismic load I want any study about this case
26 August 2021 3,594 4 View
good morning! Nowadays, I learn about docking programs and I want to try to make the muscarinic receptor with scopolamine for receptor, then I want to apply with my samples for they can work or...
21 August 2021 7,190 1 View
Hi! For some reason we need to adjust the temperature of Reverse transcription when using Superscript II. Is there a activity-temperature curve of Invitrogen Superscript II been profiled in...
04 August 2021 2,205 0 View
We are trying to spin coat PDMS on a Si substrate to achieve PDMS thickness of less than 5 microns.
24 July 2021 4,276 4 View
Hey, I try ti explain as best as possible, while being relatively new to this field. I want to perform a qPCR with the mcrA gene sequence (0.5kbp roughly) as standard. Ergo I want to quantify...
23 July 2021 587 0 View
I have heard that primary cortical neurons mature at DIV12-14. Is this true and what do people think? Has anyone added compounds to neurons younger than DIV12? I am treating neurons with...
22 July 2021 9,686 1 View
Since ancient times, herbaceous plants have been used as natural treatments for several diseases, including viral infections. Due to their concentration of potent plant compounds, many herbs aid...
19 July 2021 1,377 4 View
i need pakages for estimation VAR model using MLE
18 July 2021 8,437 5 View
In a patient undergoing corticosteroid treatment
13 July 2021 3,402 3 View
For some reason, we need to have sodium chloride (NaCl, 0.05-0.1M) in our system. Will this affect the reverse transcriptase and block the SMART reverse transcript? Thanks
18 June 2021 10,004 5 View
I'm working with very small samples and I'm wondering if I should include the on column DNAse step or leave it out. I know the columns should do a good job even without this, and I've found a few...
16 June 2021 528 3 View