A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a... | Contact experts in DNA to get answers
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Questions related to DNA
After dialysis, protein(enzyme) samples should subjected to SDS PAGE or directly protein(enzyme) samples subjected to FPLC for the protein identification.
28 October 2022 869 1 View
Hi, My antibody for immunoprecipitation is mouse IgA subtype, therefore I thought I should use normal mouse IgA instead of widespread IgG for my IP control. But I have not yet found any...
30 August 2022 9,581 4 View
Hello The Fermentor is in the form of fed batch. In primary and secondary inoculum, growth is normal, but after feeding (about 1/6 of that) about 4 hours later, The fermentor get foamy, the...
27 August 2022 3,074 1 View
Can someone explain me
10 June 2022 873 3 View
I am not getting Autodock Vina result which is showing "Configuration file parse error: multiple_occuerences" and "Command line parse error: too many positional options" I have all my ligands in...
07 June 2022 5,285 3 View
I would like to perform RAD-seq on one zooplankton with a body-size between 0.5-1cm. However, the DNA extracted from each individual by chelex can not work for library preparation. So I am...
03 June 2022 4,021 2 View
How does organic fertilizer affect the environment and ecology? Without fertilizer, soils are not as productive and crops do not grow as quickly since fertilizers give more productive soils and...
24 March 2022 9,505 6 View
I am trying to make a denaturing gel to check for total RNA integrity for subsequent RTqPCR. All the protocols I've checked so far involved using formamide which we don't have available at hand....
21 March 2022 2,595 0 View
Whether to plan experiment based on methinine & lysine content of all roughage and concentrate and per cent composition of growth tissue or other siutable methods
24 January 2022 5,516 0 View
I wish to make stable Huh7 or HepG2 cells with my gene of interest using pcDNA3.1(+). I´m using Lipofectamine 3000 kit and Geneticin selection 72h post transfection but the cells tend to loose the...
14 January 2022 3,872 3 View
> RainDrop™ Digital PCR System by RainDance Technologies, Inc. > QIAcuity Digital PCR System by QIAGEN > QuantStudio Digital PCR Systems > Prism6 by Stilla > Biorad
04 January 2022 8,133 0 View
Hi, just looking for some help with this. I can't for the life of me figure out what's causing this banding pattern. All my bands are producing this smily face pattern and neither I nor the rest...
07 December 2021 8,523 3 View
I found that knockout of my gene of interest causes higher Wnt activity (as shown by TOP/FOP flash luciferase assay as well as RNA-seq) and hence, I am trying to figure out the mechanism that...
27 November 2021 4,454 3 View
Organic fertilizer is a great solution to the negative impact of chemical fertilizer on earth and climate change. However, the efficiency of organic fertilizer is 10 to 20% of the efficiency of...
18 November 2021 5,282 23 View
Hello! I've been studying about ADAR which stands for Adenosine Deaminase Acting on RNA. I heard that ADAR only works on double stranded RNA and changes mutant A to I. But isn't mRNA in our body...
30 October 2021 3,485 3 View
I am looking for some method for phage inactivation with out damage of bacteria, for sterility test of phage preparation by USP 71. Thanks in advance
19 October 2021 5,497 5 View
Hi All, I am trying to get some colonies after the bp reaction but I have been trying this for a while and at this point I have no colonies. I am using the pDONR 221, the fragment is 2.3 kb and...
15 October 2021 1,564 1 View
CD11b+F4/80-Ly6G-CD115+should be monocytes. But what about the CD115- population? Granulocyte-Monocyte Progenitors and Monocyte-Dendritic Cell... In this paper, Fig1. Both GMP and MDP can...
12 October 2021 9,074 0 View
Hi everyone. I am trying to replicate an homology modeling protocol. Right now, I am in the TM refinement part which is made in GROMACS, but I don’t understand how to perform the following...
07 October 2021 4,729 2 View
I would like to do single cell sequencing of DNA and total RNA in the same cell . My interests on noncoding RNAs. Do you know any cmany doing that? In the publications I read about SIDR-seq. But I...
02 October 2021 4,386 1 View
Should I plan for FFPE sections or direct biopsy tissues? what is the minimum amount/diameter of tissue required for the same?
30 September 2021 8,137 0 View
Hello, I was looking forward to analyzing some samples using the Luciferase assay. However, I wanted to know how much time it would take for the sample to show results once the Luciferin enzyme is...
29 September 2021 1,378 3 View
Dear all, I want to prepare a 0.05 M, pH 7 phosphate buffer solution that contain 1% w/v casein and 0.006 M EDTA for proteolytic activity determination. As I know, EDTA and casein only dissolve in...
18 September 2021 6,703 2 View
How to prepare a solution saturated with H2S? I am looking to prepare a NACE Solution ‘A’ which is saturated with H2S in order to do sulfide stress cracking tests. I am planning to put the...
06 September 2021 4,398 12 View