A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a... | Contact experts in DNA to get answers
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Questions related to DNA
For example why Tricine - Tris gives better resolution than Glycine - Tris in this papper? https://www.nature.com/articles/nprot.2006.4 I know it is related to the ion fronts but I don't truly...
19 May 2023 1,267 3 View
Hi all. Do you know the STR profile of SK-MEL-103 melanoma cell line? I guess it is not available from ATCC... Thank you.
17 May 2023 3,931 1 View
How can I add the number at risk below KM curves in GraphPad Prism? Thank you
12 May 2023 9,050 1 View
What are the main things that can help a review article get published, even though it can be very challenging to do so?
08 May 2023 9,567 4 View
I am looking for a well-characterized melanoma cell line that is less aggressive than SK-MEL-28, and I can culture in spheroids. Ideally, commercially available at ATCC. Thank you so much!
03 May 2023 5,444 2 View
Hello, so i am scaling up a 10 L biorector to 1000 L bioreactor for enzyme production using corncob-based media. The product increased, but there's a lot of excess media left in the product. So, i...
08 April 2023 2,254 1 View
Hey, is there an easy/uncomplicated protocol to generate topological files and/or force field files from any xyz structure file, which can then be used with a force field molecular dynamics...
31 March 2023 7,376 6 View
Can someone provide me with some information about mouse vagus nerve electrical stimulation using a concentric electrode? I cannot get the concentric electrode to stimulate the nerve. I have even...
15 March 2023 2,187 0 View
I'm interested to hear opinions and anecdotes, but most useful would be recent research that gathers information from job incumbents and/or their managers. Thank you!
15 March 2023 266 12 View
Hello, My protein of interest in 104kDa. I transfected Hek293T cells with plasmid and then run western blot. The last two lane is transfected cell lysate. But as you can see I cant see a thick...
08 March 2023 5,214 0 View
After obtaining bam file, I used bcftools to perform vcf, then using bcftools consensus command line included its parameter such as refrence genome, vcf.gz file, the output file with fasta format...
05 March 2023 7,089 1 View
I want to remove non-templated nucleotides that are added to the 3' end of my cDNA by reverse transcriptases, creating blunt-ended RNA/DNA hybrids. Standard blunting protocols (T4 DNA pol./Klenow...
02 March 2023 5,084 0 View
I am working in a Molecular Biology Laboratory. I already have an UV trans Illuminator, but want to use Gel documentation system. because under transilluminator I experienced ambigious gel results...
25 February 2023 6,990 0 View
I have a batch of data for single-cell DNA sequences and I am aiming to perform an analysis of the data. Are there any existing open source packages for this mainly in R or Python? Thank you!
17 February 2023 2,916 1 View
I am going to download the 3D structure of a protein from Uniprot data bank and describe a specific part of this protein. However, for makes it more clear, I need to remove some structures (like...
13 February 2023 1,221 2 View
I have to conduct enzyme assay of around 200 samples to check their kinetic rate. Traditionally, for small number of samples we carry out cuvette based spectrophotometry assay. But for such large...
09 February 2023 3,872 3 View
Sorry if this is a basic question, but it is something that I was unsure about. I am extracting long hairpin dsRNA from HT115 E. coli for some RNAi experiments. The ssRNA from the expression...
18 January 2023 3,466 1 View
Dear fellow olfactory scientists and/or electrophysiologists, For one of my projects, I would like to stimulate/activate different primary olfactory transduction cascade stations. As a first...
17 January 2023 7,980 0 View
Has any fly person out here compared in house fly transgenesis Vs commercial services (Best Gene etc.)? How many transgenic per year would make it worth to do it in house, considering equipment...
19 December 2022 4,360 0 View
I am trying to identify the exact mechanisms , in which a plasmid get incorporated into the DNA of mammalian cell , during transfection . From literature review I found it is associated with...
12 December 2022 6,608 3 View
Hi all, I can´t seem to manage installing the plugin for MRI adipocyte tool. I have found this...
24 November 2022 2,084 0 View
After dialysis, protein(enzyme) samples should subjected to SDS PAGE or directly protein(enzyme) samples subjected to FPLC for the protein identification.
28 October 2022 876 1 View
Can we include migratory stock in the FiSAT II input for calculating growth parameters? or just the resident stock should be added.
07 October 2022 8,047 3 View
Hello Despite the many treatments that I gave, I didn't even get missfold peotein(Dimer). I see a lot of monomer on the electrophoresis gel (sometimes as a single band and sometimes as two bands),...
23 September 2022 1,917 2 View