A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a... | Contact experts in DNA to get answers
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Questions related to DNA
Hi. I want to do (renal) denervation to mice. So, I searched for some protocols and those said: "use 10% phenol". But I don't know what I have to use exactly. Do I have to use powder phenol for...
19 June 2020 3,352 0 View
A close family member of mine has limb girdel muscular dystrophy type LGMD2I 826C>A. As her type of MD is rare, it is difficult to find information on it. I would be very thankful for any...
04 June 2020 1,070 0 View
Hi all, We're doing a Ribodepletion using the Illumina Ribo-Zero Plus rRNA Depletion Kit but did not realise we had to buy RNA clean up beads (Duh). Has anyone used AMpure XP beads for RNA...
03 June 2020 8,501 4 View
what is the Idea of MRI? What are the differences between their relaxation times T1 and T2? How a superparamagnet is used for enhancing the contrast in imaging? what are the challenges for doing...
24 May 2020 728 1 View
I am curious if there exist any bioinformatics tools that can predict changes in secondary structure for proteins and/or nucleic acids. For instance, say a C-terminal loop on a protein reorganizes...
20 May 2020 2,264 8 View
I'm collecting data on dominant frequency for all tetrapods (birds, mammals, lepidosaurs, frogs, testudines, crocodiles) and I'm looking for big databases Thanks in advance!
18 May 2020 5,684 0 View
Greetings all I am looking for a way to search for specific gene(s) expression levels in an environmental transcriptome data posted on MG-RAST. If anyone has expertise in doing such it would be...
10 May 2020 2,614 0 View
Dear ALL I hope all are safe during this Covid-19 Pandemic and may god recover all from this epidemic at the earliest. What is the significance of CD4 and CD8 count on Rumninat productivity? what...
04 May 2020 3,547 0 View
I'm building VAR model using daily data. SC and HQ suggest using lag 1 for my model but i got more compelling result using lag 2. Is it appropriate to do this ?
01 May 2020 5,837 0 View
As we know STRING is a very good tool for the Protein-Protein interaction analysis. But we can analyze only 2000 protein. if it is more than 2000, is there any tool for PPI analysis.
20 April 2020 8,291 2 View
Hi guys, Am defining monocyte subsets. And classifying then to classical, intermediate and non classical. But when i am gatting non classical the CD14 expression seems smear up negative. What i...
16 April 2020 3,938 0 View
approximately, how much the body weight could increase per week in the mouse?
14 April 2020 1,115 0 View
I want to generation oligonucleotides from DNA purified by gel electrophoresis so I can use them to amplify target genes. I am thinking there exist an in-house method to breakdown DNA strand into...
08 April 2020 8,801 0 View
We have quadruplicates for mitochondria copy number multiplex PCR. ND1 representing the mitochondrial DNA and RNAse P being our reference. Equation for mitoCN= (2^(ND1 Ct-RNAseP Ct)) *...
02 April 2020 3,972 0 View
I am studying molecular docking and would like to know how to extract binding sites from literature.
25 March 2020 5,299 2 View
Phosphoric acid, when free in solution, is a triprotic acid with pKa1 = 2.2, pKa2 = 7.2, and pKa3 = 12.4. But most of the phosphate groups in DNA and RNA molecules participate in phosphodiester...
24 March 2020 5,190 15 View
Hello , i did an rflp to detect a snp for asthma and I have case and control group what is the best way to do a statistical analysis in excel to determine whether it is related to disease , my...
24 March 2020 4,104 4 View
I am having problems with solubilising a low MW chitosan (MW ~15kDa). I tried to solubilise 25 mg in 5 ml of 1% acetic acid under stirring, but the solution remained very cloudy and precipitate...
24 March 2020 7,777 6 View
Why a canonical AMBER simulation of DNA doesn't include dangling 5' phosphate? So, if I want to simulate an oligomer of length N bp, then it will have 2N bases but 2(N-1) phosphate when standard...
23 March 2020 6,306 3 View
Dear colleagues, I saw that studies usually assume a pressure difference of 1 Pa between inside and outside the building as a reference value in "real condition" (when air infiltration is driven...
23 March 2020 2,558 3 View
I want to do NMR for my G-quadruplex forming Oligonucleotides in presence of a specific metal ion. Our lab is new to this topic and no one in our lab has experienced about this.I have tried using...
18 March 2020 8,571 2 View
Dear researcher, I am extracting DNA from IV plants which contain a lot of phenolic compounds and secondary metabolites, due to which the DNA extracted always contains impurities (I tried several...
06 March 2020 5,149 5 View
Hello, I'm currently working on DNA extraction from E. coli suspended in PBS. I'm working with concentrations ranging from 102 CFU/mL to 105 CFU/mL (and 108 CFU/mL as a positive control) and I...
03 March 2020 7,703 14 View
I would like design a drilling cuttings uplifting model in an ambient conditions. So, what the things i have to cover to design the model? Consideration: 1. Drilling Fluid (Water based mud) 2....
03 March 2020 7,176 6 View