A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a... | Contact experts in DNA to get answers
1,250 views 3,169 posts
Questions related to DNA
Hi all, What are the possibilities of a oxidized nucleobase, getting paired with the complementary base. does it satisfies Chargaff rule ? Can 8-Oxo-Guanine be paired with Cytosine ?
01 January 2019 875 4 View
I am trying to amplify by PCR a fragment (2kb) from a DNA template which is the product of MDA reaction. This MDA reaction is previousliy performed with plasmids as template and all of them...
01 January 2019 3,972 1 View
I have an DNA insert that I intend to order as a complete double stranded construct and clone into a vector using restriction enzymes, however I am unsure exactly how to design the restriction...
01 January 2019 9,888 3 View
If I have part of the DNA, of a tyrannosaur and complete it with the DNA found from another tyrannosaur, can we be able to clone it on the mother cells of a Crocodile?
01 January 2019 4,957 9 View
rRNA and genes that encode RNA proteins, although highly conserved, are used for phylogenetic analysis of distant species. Why is tRNA not recommended for determining phylogenetic relationships?
01 January 2019 8,933 7 View
So proteins bind to DNA or genes all the time. I wanted to know whether before binding with a protein, the gene needs to be activated or not. If there is a research article backing your claim,...
01 January 2019 1,269 3 View
Which method do you recommend for the estimation of growth parameters mortalities and exploitation rate for deep Mediterranean fish using Length frequency distribution? What do you think about...
31 December 2018 6,423 0 View
I face many difficulties in gel purifying the restricted products A260/280 ratio is high sometimes Failure to recover DNA from gel ( completely) Loss of DNA - lesser yield So is there any other...
12 December 2018 8,951 22 View
Biofilm is a structure built by a community of bacteria. When the density of one type of bacteria is high, all the other bacteria sense that. At a certain point in the population density of those...
12 December 2018 1,214 1 View
How long I can store DNA that has been digested with restriction enzymes, gel purified and eluted in elution buffer? Will the overhangs be stable (since ss DNA are unstable) after freezing in -20...
12 December 2018 486 0 View
Are there really, Evolution is continuous as long as this relationship persists. When the relationship is intercepted, the population goes extinct.
12 December 2018 4,243 28 View
I have purified my PCR product with PCR purification kit by thermo scientific. When analysing the sample in nanodrop after purification I get a very high absorbance at 230 nm. Will this interfere...
12 December 2018 9,448 8 View
I have used this PCR protocol before and has worked beautifully! Now the results are just very smeary. I suspect the smear is coming from too much DNase activity and DNA degradation? I have tried...
12 December 2018 1,939 31 View
Hi Guys, I have been doing heat shock transformation with BL21AI cells for the last one month, But the transformation efficiency is consistently extremely low. I tried making chemically competent...
12 December 2018 4,098 6 View
Generally during the transportation, DNA is often degraded, specially the High Molecular Weight DNA which is extremely fragile to external shocks. I am looking for suggestions/recommendations...
12 December 2018 7,499 24 View
i wonder that logic of bioinformatic tools of ''protein to dna''. if i want to make hybridization probe for interested amino acid sequence, what are the steps that i have to do ?
12 December 2018 7,279 0 View
I looked up a gene on Ensembl (https://useast.ensembl.org/index.html) to get its sequence. It states that the gene is located on the reverse strand. Is the sequence of nucleotide bases given 5' to...
12 December 2018 6,418 2 View
An R script or a link to sample code would be highly appreciated.
12 December 2018 8,401 0 View
I am trying to prepare hyaluronic acid - PEG hydrogels to which DNA (1200bp) is covalently coupld by copper-free azid click chemistry. Prior to gelation, initial precursor mixture including DNA,...
12 December 2018 7,194 1 View
Could anyone help me find the best composition for cell lysis solution for manual DNA extraction using blood as the source? I want to prepare the solution in house. Does anyone have experience...
29 November 2018 1,662 2 View
I would like to know from your experience where the mistake is Experimental steps include the use of sodium salt calf thumus and was diluted in TE buffer size 50 ml and at a concentration of 2 mg...
11 November 2018 5,981 5 View
Dear colleagues I need for knowing how avoiding shrink of PCR tube through PCR Technique...for knowing the manufacturer of thermocycler (prime). Thanks previously
11 November 2018 8,755 0 View
We are using DNA collection kits for saliva samples from DNA-GENOTEK company and the product is Oragene-500. However, they purchased new product named OG-600 and I wonder what is the major...
11 November 2018 7,697 3 View
I am currently weighing the pros and cons of two methods for assessing the dietary preferences of a large carnivore, the spotted hyena. I have previously used microscopic hair root analysis (based...
11 November 2018 5,712 10 View