A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a... | Contact experts in DNA to get answers
1,515 views 3,546 posts
Questions related to DNA
The HGP project utilized white blood cells from female and sperm cells from male candidates to map the human DNA. Since these cells are differentiated, the ratio of heterochromatin to euchromatin...
10 October 2019 5,577 1 View
I'm using a stable sole of TiO2 in a acidic environment and I've tried to dilute it by ethanol and deionized water. but every time I see pin holes. what can I do? thanks
17 September 2019 7,255 6 View
What is the best method/software/script to remove all the protein sequences which dont have a trypsin cleavage site (R or K amino acids) from a fasta file?
16 September 2019 9,765 1 View
I'm trialling a recently described salting out protocol to extract HMW DNA from insect tissue: https://www.biorxiv.org/node/716332.external-links.html. The yield so far is good (~2-6 ug DNA / 30...
09 September 2019 4,466 6 View
To begin my words, I will show my assumption about the oligonucleotide process. 1. Drying process itself doesn't influence to oligonucleotide quantity. 2. The OD value should be same between first...
09 September 2019 8,071 4 View
I want to collect saliva samples in the Zymo DNA RNA shield in order to extract human DNA and RNA from the saliva. Has anyone tried this, either DNA, RNA or preferably both, and was the quality...
09 September 2019 7,882 1 View
I need some help with this issue. Anyone have arranged protocol? I understand that the first step is the separation of plasma from WBC+RBC and after extraction DNA and qPCR, but it's still unclear...
09 September 2019 1,023 6 View
Hello, I want to simulate multiple interactions between DNA in solution(Ion, pH, Temperature) and plasmonic field. (Ion, pH, Temperature => DNA dimension(Rg, persistence length) and...
09 September 2019 2,609 1 View
I'm running my PCR product on 1% gel (to excise for gel purification, samples to be sequenced). My target amplicon is 1090bp, and I can reliably get a strong, clean band with few non-specific...
09 September 2019 3,851 9 View
my project involve addition of lysine to the anti-fungal agent epoxyconazol , which method that can help me ? which type of chemical reaction ? can someone help me?
09 September 2019 5,947 0 View
Good day, currently I am practicing extracting total DNA using drosophila melanogaster (fruit fly) adult as well as larvae as my sample with Qiagen DNeasy Blood and Tissue Kit. However, I keep...
29 August 2019 686 3 View
I am going to observe the dynamic change of TAM in B16 melanoma, my current gating strategy is CD11bintF4/80hi, but the cell cluster is not very clearly. Is this strategy accurate enough? How to...
26 August 2019 9,399 4 View
I have some DNA extractions that I would like sequenced to do some population genetics work (GBS or similar). Unfortunately, due to the age of the samples and their storage method (stored in...
14 August 2019 8,565 3 View
08 August 2019 5,170 3 View
Hi! Im trying to calculate ion strength and pH of solution. Components of the solution: 47 ul of 1000 pmol DNA in Elution Buffer (10 mM Tris-HCl pH 8.5) was bound to 3 ul of 10 µM YoYo1...
08 August 2019 3,522 8 View
I tried making stock solution of 100 mg/ml of Ampicillin from anhydrous Ampicillin from Alfa Aesar. I mixed Nuclease free water to 5 g of anhydrous Amp to make it 50 ml. However, it wasn't...
08 August 2019 1,744 7 View
Did any body know the differences between Resinferatoxin and capsaicin? Both are used to denervate the sensory nerves .
07 August 2019 1,833 1 View
I am currently planning an experiment making an antibody-siRNA conjugate using this kit: TriLink Antibody-Oligonucleotide All-in-One Conjugation Kit,...
30 July 2019 3,173 3 View
how do i delete this question, not required
30 July 2019 9,101 1 View
Does a paired-samples mean one to one matching of the data between pre-and post tests or is it just the pre- and post- data from the same group taken for the comparison of the mean difference?
29 July 2019 6,552 7 View
The relation of increased CSF pressure and optic nerve is well known. What about other cranial nerves? Can changes in CSF pressure cause symptoms by effects on their intracranial course?
29 July 2019 4,862 43 View
The type of tumor is schwannoma - benign tumor arising from the nerve sheath (covering) of cranial nerve(5th cranial for me) alongside the brain stem. Generally slow growing tumors that occur on...
29 July 2019 2,383 0 View
Bo maps acquired according to the svFieldMap pulse sequence (Bruker, Paravision 6.0) should give as an intensity per pixel the offset of Bo in Hz. However, when converting the 2Dseq files (32 bit...
23 July 2019 4,328 3 View
It is a well- known result of population genetics that admixture, heterogeneity, or stratification in a population can make it impossible to draw valid conclusions from a conventional case-control...
22 July 2019 6,317 2 View