I get a fungal culture on a liquid medium (czapeck). When i try to recuperate spores, they couldn't be separated from mycelium.
@Abdelilah Chakhmani
The liquid culture surface grown mycelium with conidia should be dispersed in Tween-saline solution (0.1% Tween-80 in 0.8% NaCl) and mix well to get the conidia in solution. After this, filter this mixture through cotton (glass funnel with cotton to be autoclave and keep ready), collect the conidia in sterile vessel, you will only get the conidia, devoid of mycelia or any large particles.
Grow culture on MEA as indicated above and then separate spores by adding Tween 20. If you don't have Tween 20 0.2- to -.8% you can do SDS 0.2%. You can then isolate with 1mL micropipette.
It is important that you transfer an aliquot of the culture that you have already obtained to a liquid culture added with Tween, vortex it for 1 minutes and remove the supernatant with a micropipette, this way you will be able to catch the spores you want. Luck!
At first you grown the fungal culture on agar plate with preferred medium. Then after the formation of spore, you add sterilized saline buffer to that plate. Now just rubbed the fungal mycelium smoothly with the help of sterilized needle for getting the spore in buffer. Now, you just put off buffer from the plate with the help of micropipette and pass through the sterilized 3/4 fold gauze to get mycelium free spores.
- Badges
- Science topic
- Similar topics
- Biological Science
- Molecular Biology
- Biomolecules
- Nucleic Acids
- DNA