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Questions related to Nucleic Acids
I characterized functional groups of EPS using FTIR. Goups of sugars and proteins (e.g., -OH, amide, -COOH) as well as phosphate group of nucleic acid were readily detected. However, peaks of...
06 February 2021 4,987 1 View
Once I have oligo-GNP conjugates, I need to use them as sensor for my target nucleic acid. Before doing that I need to confirm whether the oligo probe (conjugated to GNP) hybridizes well with my...
05 December 2020 5,140 2 View
I want to do an electrophoresis of RNA from baker's yeast. However, our lab only has a vertical electrophoresis, while usually electrophoresis of nucleic acid (RNA or DNA) use horizontal...
30 November 2020 8,341 5 View
I am doing nucleic acid extraction (manual silica magnetic bead-based) from the serum sample. After the extraction, I have taken the OD 260/280 in UV spec. There is no protein contamination. But...
05 November 2020 1,557 2 View
I am trying to find out the presence of short repeats (both direct and inverted) in a 3-4 kb long DNA fragment. Although I had tried with a few free online tools, the results were not...
17 October 2020 8,762 3 View
I want to measure protein and nucleic acid within conditioned medium. I used nanodrop with fresh medium as blank. Yet, I got a weird result, the blank absorbance is higher than the sample....
12 October 2020 3,504 3 View
I need to isolate DNA from an old animal skin cell. But before going for isolation of DNA. I need to clarify that the DNA is denatured or not? Please recommend me a method for that.
02 October 2020 8,626 2 View
Hello, I would like to know as many as possible commercially available nucleic acid extraction kits that DO NOT require thermal incubation after the lysing solution is added to the sample (or...
10 September 2020 8,660 5 View
Hybridization chain reaction (HCR), a form of non-enzymatic method for amplification of target nucleic acids. I need to design the two HCR primers. Is there any special software for doing that?
28 August 2020 1,337 1 View
I am considering to use nucleic acid stain to evaluate how much viruses are recovered after concentration of wastewater by ultracentrifugation. My target is virion not free viral nucleic acid....
09 August 2020 576 6 View
Aptamers in most cases are themselves nucleic acids, generally used to target other bio-molecules. I wonder if we can design aptamers in such a way that they target other nucleic acids. Will...
07 June 2020 3,519 1 View
How to perform nucleic acid test? Please do provide information in easy and convenient way.(from beginning) Thanks in advance
29 March 2020 2,487 3 View
I am looking found reference about people that has been working the proposed method in this publication: "Nucleic acid purification from plants, animals and microbes in under 30 seconds" from Zou...
11 March 2020 1,244 2 View
Hi All, I've been trying to use the MagMAX Cell-Free Total Nucleic Acid Kit from Thermo to isolate total nucleic acid from plasma, and the plasma was collected from tumour patients. However,...
04 March 2020 4,503 2 View
03 March 2020 3,250 1 View
I am working on bacterial killing by the antibacterial agent and want to know the mechanism of cell death in bacteria. bacteria show death by cell membrane damage and what will be the probable...
02 February 2020 6,236 3 View
Hi, I am working on t24 cell line. Before this, I did RT-PCR from cells that I cultured on 10mm dish. The RNA extraction was done using Trizol method and the nucleic acid concentration was...
01 January 2020 9,654 4 View
During nucleic acid extraction after precipitation with 100% ethanol, we wash the pellet with 70% ethanol to remove excess salts from the pellet. But during these washes, nucleic acid can also...
27 December 2019 5,962 7 View
Hi everyone, I am purifying a his-tagged transcription factor using Ni-NTA agarose. After eluted from the column, 260/280 could reach to 1.9 or more. I am sure that the protein can be expressed....
12 December 2019 7,534 8 View
12 December 2019 5,597 7 View
Hi, I'm attempting to determine the conjugation efficiency of a reaction between nucleic acid aptamers and PEG-maleimide. My idea was to separate free aptamer from the conjugated PEG using a...
10 October 2019 6,824 3 View
Hi, I'm Kaniz, PhD student at university of Leeds. I'm working with HBc VLPs to generate different vaccine scaffolds which are expressed in E. Coli. I have found that the HBc VLPs contain a good...
10 October 2019 1,318 4 View
Hi, I am performing a reaction in which i'm conjugating DNA oligonucleotides to an 8-arm PEG-Maleimide. I want to quantify the reaction efficiency by measuring the amount of nucleic acid remaining...
10 October 2019 4,443 1 View
I use liposomes as a vehicle for gene silencing....so after encapsulation of my siRNA-protein polyplexes I measure what is left outside the liposomes using Quantiflour RNA kit (promega). I have a...
09 September 2019 1,972 3 View