Hello i'm studying sequence relationship between species of fungal isolates. I find it difficult to establish which phylogenetic tree analysis is required (rooted or unrooted tree). Need some enlightenment.
Hi,
an unrooted phylogenetic tree means that the common ancestor is not shown. This could be useful for phylogenetic analysis regarding different organisms/species and the relationship that they might have.
A rotted phylogenetic tree is instead showing a common ancestor, indicating the relationship between the (at least two) organisms/species and their relationship.
You should use the unrooted tree when you are trying to understand the conservancy/diversity in the sequences that you are analysing.
For more info please check the following link:
https://brainly.in/question/3769990#:~:text=Rooted%20trees%20shows%20the%20most%20basal%20ancestor%20of%20the%20tree.&text=Unrooted%20phylogenetic%20tree%20does%20not%20show%20an%20ancestral%20root.&text=Unrooted%20trees%20represents%20the%20branching,of%20organisms%20without%20indicating%20ancestry.
https://www.researchgate.net/post/What_if_anything_does_an_unrooted_phylogenetic_tree_mean
http://www.bioinf.man.ac.uk/resources/phase/manual/node59.html
Best regards
Agreeing with answers provided by the researchers previously, I am adding my view. In this context, the rooted phylogram should be generated for microorganisms (bacteria, fungal strains and viruses) with medical consequences. This is relevant to mention that medically important bacteria are strains and been categoriszed based upon sero-diagnosis. I mean the Ag and its cognate antibody interaction. e.g. ETEC (Enteropathogenic E.coli), EPEC (Enteropathogenic E.coli) , EHEC (Entero haemorrgic E. coli) etc.,if the sequences will be generated for phylogenetic analyss, all the sequence have a common origin is E.coli. More so, the all the 16 s r DNA will be same for all the sequences. The variation will be at differential virulent gene expression, when draft or WGS will be made. The medically important fungal strains also expose different antigens, at different stages of disease development. Hence, I suggest for a rooted phylogram for fungal strains, like Trichophyton mentagrophytes, Epiderophyton floccossum, which are clinically isolated.
Since you are studying the sequence relationship between/among species of fungal isolates, the unrooted tree is relevant. A rooted tree is required to establish the evolutionary relationship. wherein the group of organisms in the panel of your study is needed to be linked to an outlier in the already known evolutionary tree so that the evolutionary relationship is established in consensus with the other studies.
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