A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a... | Contact experts in DNA to get answers
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Questions related to DNA
What are the differentiating factors between PyTorch and TensorFlow, two major Python libraries for Machine Learning, in terms of flexibility, ease of use, and industry adoption?
11 August 2023 4,686 4 View
I got DNA as 500 to 2300 ng/ul with 1.5 260/280 ratio but still the color of the DNA is brown , how to remove this brown color?? I need this DNA for NGS analysis whether this is good for NGS...
12 July 2023 7,884 2 View
Please tell us process of protein aggregation.
10 July 2023 2,695 1 View
There is difference between plagiarism softwares employed by journals and the free websites that are available online. which website is closest to professional plagiarism checker softwares ? when...
07 July 2023 1,769 1 View
I have diluted an E.coli sample of 1*10^8 CFU/mL to 1 CFU/mL by serial dilution process. Now, I have a doubt that how many E. coli bacteria will be present in that 1 CFU/mL solution. It would be...
05 July 2023 9,563 0 View
I have some clinical samples of human synovial fluid and I am extracting the bacterial DNA. I was wondering it is possible to spin the samples to separate the bacterial cells from the human cells...
14 June 2023 439 4 View
Our gel with too combs is too big for the imager so we cut it into four parts. Now only one piece has a positive and negative control (but the run and electrophoresis are the same). Can I crop the...
08 June 2023 8,984 1 View
During the sample preparation of cell lysate, add cell lysis buffer, agitate the cells, centrifuge, and recover and aliquot supernatant. What is the procedure/protocol for cell pellets preparation...
07 June 2023 5,261 3 View
I’m working on recombinant protein expression. My bacteria lysis protocol: The grown cells (Bl21) were harvested by centrifugation at 5000g for 20 min at 4 ◦C, resuspended in 5mL of PBS buffer,...
31 May 2023 1,291 3 View
For example why Tricine - Tris gives better resolution than Glycine - Tris in this papper? https://www.nature.com/articles/nprot.2006.4 I know it is related to the ion fronts but I don't truly...
19 May 2023 1,254 3 View
Hi all. Do you know the STR profile of SK-MEL-103 melanoma cell line? I guess it is not available from ATCC... Thank you.
17 May 2023 3,916 1 View
What are the main things that can help a review article get published, even though it can be very challenging to do so?
08 May 2023 9,550 4 View
I am looking for a well-characterized melanoma cell line that is less aggressive than SK-MEL-28, and I can culture in spheroids. Ideally, commercially available at ATCC. Thank you so much!
03 May 2023 5,429 2 View
Hello, so i am scaling up a 10 L biorector to 1000 L bioreactor for enzyme production using corncob-based media. The product increased, but there's a lot of excess media left in the product. So, i...
08 April 2023 2,241 1 View
Hey, is there an easy/uncomplicated protocol to generate topological files and/or force field files from any xyz structure file, which can then be used with a force field molecular dynamics...
31 March 2023 7,365 6 View
Hello, My protein of interest in 104kDa. I transfected Hek293T cells with plasmid and then run western blot. The last two lane is transfected cell lysate. But as you can see I cant see a thick...
08 March 2023 5,199 0 View
After obtaining bam file, I used bcftools to perform vcf, then using bcftools consensus command line included its parameter such as refrence genome, vcf.gz file, the output file with fasta format...
05 March 2023 7,078 1 View
I want to remove non-templated nucleotides that are added to the 3' end of my cDNA by reverse transcriptases, creating blunt-ended RNA/DNA hybrids. Standard blunting protocols (T4 DNA pol./Klenow...
02 March 2023 5,072 0 View
I am working in a Molecular Biology Laboratory. I already have an UV trans Illuminator, but want to use Gel documentation system. because under transilluminator I experienced ambigious gel results...
25 February 2023 6,980 0 View
I have a batch of data for single-cell DNA sequences and I am aiming to perform an analysis of the data. Are there any existing open source packages for this mainly in R or Python? Thank you!
17 February 2023 2,894 1 View
I have to conduct enzyme assay of around 200 samples to check their kinetic rate. Traditionally, for small number of samples we carry out cuvette based spectrophotometry assay. But for such large...
09 February 2023 3,860 3 View
Sorry if this is a basic question, but it is something that I was unsure about. I am extracting long hairpin dsRNA from HT115 E. coli for some RNAi experiments. The ssRNA from the expression...
18 January 2023 3,447 1 View
I am trying to identify the exact mechanisms , in which a plasmid get incorporated into the DNA of mammalian cell , during transfection . From literature review I found it is associated with...
12 December 2022 6,596 3 View
Hi all, I can´t seem to manage installing the plugin for MRI adipocyte tool. I have found this...
24 November 2022 2,032 0 View