A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a... | Contact experts in DNA to get answers
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Questions related to DNA
I used 3-HPA as matrix (10mg/mL in 50% ACN water solution with 1 mg/mL ammonium citrate dibasic) , and the sample (~60 pmol, 30-mer, and 10 of 30 were phosphorothioated ,~ 9.4 kDa) was analyzed...
07 July 2019 1,261 4 View
I am currently planning an experiment making an antibody-siRNA conjugate using this kit: TriLink Antibody-Oligonucleotide All-in-One Conjugation Kit,...
07 July 2019 3,014 2 View
Im having issues with interpreting the CV graph produced by my experiment. Here are the details: Metrohm spe gold disposable electrodes. Chemically cleaned then electrodeposition of palladium...
07 July 2019 6,149 3 View
Dear professional experimental masters, As you can see from the schematic diagram (front view of a gel, white part is the DNA which showed on blue light after staining), there is a problem of the...
07 July 2019 5,219 9 View
I am formulating an herbal topical cream for nerve pain. Herbal extracts and essential oils with traditional use and formal data support are included. I wanted to include a B-complex for nerve...
03 July 2019 3,077 2 View
I am knocking in a DNA fragment with homology to the E. coli genome via a lambda red knock-in protocol. I induce the cells to express lambda red genes during competent cell preparation. The...
30 June 2019 340 4 View
I would like to denervate mice skin sympathetically and I will use 6-OHDA. I will be appreciated if you help me to know how can I be sure that the nerves are removed. i found that it can be known...
17 June 2019 6,171 0 View
need a good and emerging answer... Thanks
10 June 2019 1,932 8 View
one more question, at what condition DNA can be denatured? Thanks in advance
06 June 2019 1,734 4 View
I have a set of sulfonyl compounds and i would like to know if they have any interaction with DNA. How should i choose the target DNA?
06 June 2019 3,077 5 View
Hi This is very odd. Whenever I come back to the plate and defrost it (At room temp for a couple of hours) wells that I know had previously been filled have become void. Some of them I have now...
06 June 2019 9,109 4 View
Hi, In most of the DNA biosensor experiments and articles they have used MCH (6-Mercaptohexanol) as a blocking agent, to orient DNA 90o and to remove non-specifically bound from the...
28 May 2019 3,116 1 View
How many Research-gate Professors/scientists/ scholars are EVOLUTIONISTS OR ANTI- EVOLUTIONISTS? YOUR RESPONSE WOULD BE HIGHLY APPRECIATED. My Professor who is an Evolutionist told me that...
05 May 2019 1,205 4 View
05 May 2019 5,864 1 View
I have downloaded a sequence of 16S rRNA gene of Staphylococcus aureus from online database SILVA (https://www.arb-silva.de). While the DNA sequence of any gene is supposed to have 'Ts' instead of...
05 May 2019 6,075 5 View
Virus is living only inside living host whereas outside the host , it remains inactive but not dead Chemically it is a protein in combination with DNA or RNA Can it be possible to destroy or kill...
05 May 2019 2,458 6 View
Iam working on diet analysis of carnivores with special reference to the evaluation ofpredationpressure on wild ungulates?
19 April 2019 6,227 3 View
Hello everyone, I hope you will understand what I want to ask. For exemple: I have 500 genomic DNA samples (different genotypes of same plant species) and I have 10 pairs of primers (Fwr+Rwr). I...
04 April 2019 4,198 6 View
What's the difference and which is correct?
04 April 2019 1,110 4 View
I've read that the following formula: (G3+)(N1-7)(G3+)(N1-7)(G3+)(N1-7)(G3+) is used to algorithmically search for potential g-quadruplex motifs in various genomes. I'm wondering whether certain...
11 March 2019 6,275 2 View
I know the equation is Coverage = (read length) (number of reads) / (haploid genome length) but am a little uncertain on what to put in each field (namely read #). I have an organism with a...
03 March 2019 725 6 View
I know Qiagen makes plant specific extraction kits, but has anyone tried the DNeasy blood and tissue kit with plant material? Thanks, Matt
03 March 2019 3,748 2 View
All study that Mitochondrial DNA inherited from mothers only. While, Recently, there are publication suggest that Mitochondrial DNA can be inherited from fathers, not just mothers( fill attach at...
03 March 2019 5,910 15 View
The purities of my genomic DNA samples range between 1.51 and 1.71 at 260/280nm wavelengths. This is the kit: https://www.zymoresearch.com/products/onestep-qmethyl-pcr-kit
03 March 2019 8,195 3 View