I am working on MSCs culture and to normalise the data, I am doing Picogreen DNA quantification. However, the total amount of DNA is downgraded by 30% when I repeated the quantification using stored cell lysate after a week time (-80 degree) and after 2 weeks, further decline was observed. So, is this trend is normal or quantification on the fresh cell lysate is always better to avoid this kind of change in data. Please share your opinions. Thanks
I am not sure how you are lysing the cells, but if DNAses are present then rapid degradation is to be expected. It is possible to obtain highly pure DNA that is stable at -80C with minimal degradation.
For your experiments, you may wish to always use fresh cell lysate if you don't want to solve the storage problem.
Erik Jue Thanks for your input. I was using passive lysis buffer to break down the tissues.
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