A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a... | Contact experts in DNA to get answers
1,254 views 3,169 posts
Questions related to DNA
I've been trying to get DNA from unknown bacteria to show up on an agarose gel. I used the QIAprep spin mini prep kit on some unknown bacteria, so I should have gotten plasmid DNA. Using a nano...
08 December 2020 8,677 10 View
I am usiing LAMP kit, specifically designed for liriomyza huidobrensis. I have a random samples of differet insects and LAMP seems to amplify insects from other Genus as well. Although, other...
08 December 2020 5,767 3 View
Hello, I have been trying to ligate a 1.4kb insert in the pENTR D-TOPO plasmid using different molar ratios like 1:1, 2:1, and 3:1 (recommended by NEB) , but every time no colonies are seen. Only...
08 December 2020 3,145 5 View
My lab have some skin biopsies that were previously conserved in paraffine , and i wanted to know if it is possible to extract DNA or RNA from them.
07 December 2020 8,090 6 View
One of the steps during the preprocessing of the data from metatranscriptomic analysis is to remove any host reads (host contamination) by comparing to host database. But what if there is no host...
01 December 2020 3,458 2 View
Hi I'm interested in the production of insulin at a lab-scale. I read some papers about the topic. One of them mentioned autocleavage of the proinsulin without any enzymatic cleavage. But they...
28 November 2020 2,929 3 View
Hello! I'm using Ultra-Deep Microbiome Prep kit for the isolation of DNA from mice skin biopsies. I was doing it yesterday for the first time and I didn't achieve to any DNA yields. So I would...
20 November 2020 5,700 1 View
Why the number of secondary depressions on existing chromosomes varies?
16 November 2020 9,813 3 View
I would like to know whether some specific biomolecules will also evaporate and form vapors along with solvent vaporization. If so, what kind of biomolecules?
20 October 2020 5,815 7 View
Hi, I am working on the relationship between tissue damage repair and cell pyroptosis. The cell model I used were THP-1(human) and BMDM (mouse). This is the way I use to induce THP-1 cell...
14 October 2020 1,565 0 View
Hello i'm studying sequence relationship between species of fungal isolates. I find it difficult to establish which phylogenetic tree analysis is required (rooted or unrooted tree). Need some...
07 October 2020 7,600 4 View
Is 8th week the earliest week where we can determine the fetal sex ?
06 October 2020 9,690 9 View
I get a fungal culture on a liquid medium (czapeck). When i try to recuperate spores, they couldn't be separated from mycelium.
25 September 2020 7,010 6 View
What is the general consensus on one vs. two-tailed hypothesis testing in planned contrasts? I have a repeated measures mixed design. The study consists of three groups (A, B, C) and we have three...
18 September 2020 8,302 12 View
Researchers around the world are doing their best to perform experiments but when they want to publish their research work in gold open access journals, APC will stand infront of them like a wall....
17 September 2020 4,116 10 View
I am preparing CTAB buffer and I read that addition of PVP (Polyvinylpyrrolidone) helps to remove phenolic compounds. Most of the times in protocols there is no additional information about...
06 September 2020 7,696 4 View
I am working on MSCs culture and to normalise the data, I am doing Picogreen DNA quantification. However, the total amount of DNA is downgraded by 30% when I repeated the quantification using...
27 August 2020 5,283 2 View
I have some plasmids with sacB. Ecoli carrying these plasmids seems slow even on standard LB without sucrose. I sequenced the plasmids and everything seems normal. Normally I have nice colonies...
23 August 2020 3,411 3 View
Normally, ecoli miniprep cultures are grown for around 12-18 hours at 37C. However, due to my schedule, I won't be able to harvest the cultures for >24 hours. I think >24 hours is too long to...
18 August 2020 7,988 6 View
I am using TissueLyser LT to extract DNA from plant tissue ( without using liquid nitrogen cause it's not available ) but I do not know how much time is required? I did this in 6 minutes at 50HZ...
16 August 2020 9,393 7 View
What would be the acceptable CV% and SD for copy number results between stool samples in qPCR (same group)? I am evaluating different bacteria (E.coli, Lactobacillus, etc.) using the absolute...
07 August 2020 8,233 4 View
What is a good statistical test to investigate how frequency distribution responses are influenced by more than one predictor variable? Can this be done within the multiple linear regression or...
06 July 2020 6,722 0 View
Hi all, We're doing a Ribodepletion using the Illumina Ribo-Zero Plus rRNA Depletion Kit but did not realise we had to buy RNA clean up beads (Duh). Has anyone used AMpure XP beads for RNA...
03 June 2020 8,447 4 View
I am curious if there exist any bioinformatics tools that can predict changes in secondary structure for proteins and/or nucleic acids. For instance, say a C-terminal loop on a protein reorganizes...
20 May 2020 2,112 8 View