A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a... | Contact experts in DNA to get answers
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Questions related to DNA
Hi everyone. I am trying to replicate an homology modeling protocol. Right now, I am in the TM refinement part which is made in GROMACS, but I don’t understand how to perform the following...
07 October 2021 4,743 2 View
I have troubles quantifying my tau aggregates"purified" by sequential protein extraction from HEK293 cells, especially with the sarkosyl-insoluble fraction. The pellet was resuspended in 1x PBS,...
07 October 2021 8,150 2 View
I would like to do single cell sequencing of DNA and total RNA in the same cell . My interests on noncoding RNAs. Do you know any cmany doing that? In the publications I read about SIDR-seq. But I...
02 October 2021 4,398 1 View
Should I plan for FFPE sections or direct biopsy tissues? what is the minimum amount/diameter of tissue required for the same?
30 September 2021 8,149 0 View
Hello, I was looking forward to analyzing some samples using the Luciferase assay. However, I wanted to know how much time it would take for the sample to show results once the Luciferin enzyme is...
29 September 2021 1,392 3 View
Dear all, I want to prepare a 0.05 M, pH 7 phosphate buffer solution that contain 1% w/v casein and 0.006 M EDTA for proteolytic activity determination. As I know, EDTA and casein only dissolve in...
18 September 2021 6,714 2 View
How to prepare a solution saturated with H2S? I am looking to prepare a NACE Solution ‘A’ which is saturated with H2S in order to do sulfide stress cracking tests. I am planning to put the...
06 September 2021 4,417 12 View
I isolated mRNA from HEK293T cells using oligo dT cellulose, followed by RNA Cleanup and Concentrate kit that includes a DNAse digest. The final mRNA yield was about 6.7ug, with 260/280 and...
26 August 2021 1,629 5 View
Hi! For some reason we need to adjust the temperature of Reverse transcription when using Superscript II. Is there a activity-temperature curve of Invitrogen Superscript II been profiled in...
04 August 2021 2,128 0 View
We are trying to spin coat PDMS on a Si substrate to achieve PDMS thickness of less than 5 microns.
24 July 2021 4,220 4 View
Hey, I try ti explain as best as possible, while being relatively new to this field. I want to perform a qPCR with the mcrA gene sequence (0.5kbp roughly) as standard. Ergo I want to quantify...
23 July 2021 548 0 View
In a patient undergoing corticosteroid treatment
13 July 2021 3,354 3 View
For some reason, we need to have sodium chloride (NaCl, 0.05-0.1M) in our system. Will this affect the reverse transcriptase and block the SMART reverse transcript? Thanks
18 June 2021 9,942 5 View
I'm working with very small samples and I'm wondering if I should include the on column DNAse step or leave it out. I know the columns should do a good job even without this, and I've found a few...
16 June 2021 467 3 View
I was wondering whether it is possible to turn off the expression of a transcription factor that is expressed by CD8 T cells, and if yes, how?
04 June 2021 8,209 1 View
I'm trying to get anhydrous formamide for dissolving inorganic salts(LiH, NaNH2) in the glove box, but it is hard to find commercial anhydrous formamide. Thus I'm looking for how to drying...
04 June 2021 4,458 2 View
I have been using electrochemical techniques recently for a project and I have observed that the concentration of ferricyanide is always taken more than ferrocyanide. Also, oxidation potential is...
24 May 2021 7,672 9 View
Hi all, I am posting our paper on medrxiv. I have never come across Distribution/Reuse Options to choose from and those look copyright-related. It reads: © No reuse/adaptation without...
20 May 2021 4,561 4 View
During the energy minimization of a protein before Molecular Dynamic Simulation in GROMACS software, I am getting this error: "One or more water molecules can not be settled in GROMACS". Just...
18 May 2021 4,420 10 View
I am working with mouse primary cortical neurons that are plated in a 96-well plate. I treat the cells with a ranging drug concentration for 24 h and then measure LDH using a kit to determine...
18 May 2021 722 3 View
I am looking for a non-NMR based (preferably) method of analyzing glycogen synthesis in the skeletal muscle and heart. Can anyone share a protocol where one has used radiolabeled glucose...
18 May 2021 7,938 2 View
waste water treatment
14 May 2021 6,782 9 View
We aim to cleave our DNA probe from a AP site (apurinic/apyrimidinic site) just rising up the temperature. What is the lowest temperature required to realise the cleavage, 50, 60, 70, 80, or 90...
11 May 2021 124 5 View
Hello, It's been a while since I've come across this idea of abortive rnas having a function other than instant repression, or at least, 'function' regarding the initiation of transcription....
05 May 2021 3,577 4 View